Applications of immunoPET: Using 124I-anti-PSCA A11 minibody for imaging disease progression and response to therapy in mouse xenograft models of prostate cancer

Knowles, Scott M.; Tavare, Richard; Zettlitz, Kirstin A.; Rochefort, Matthew M.; Salazar, Felix B.; Jiang, Ziyue Karen; Reiter, Robert E.; Wu, Anna M.

doi:10.1158/1078-0432.ccr-14-1452

Applications of immunoPET: Using ¹²⁴I-anti-PSCA A11 minibody for imaging disease progression and response to therapy in mouse xenograft models of prostate cancer

Journal Article · Fri Oct 17 00:00:00 EDT 2014 · Clinical Cancer Research

DOI:https://doi.org/10.1158/1078-0432.ccr-14-1452· OSTI ID:1345504

Knowles, Scott M. ^[1]; Tavare, Richard ^[2]; Zettlitz, Kirstin A. ^[2]; Rochefort, Matthew M. ^[2]; Salazar, Felix B. ^[2]; Jiang, Ziyue Karen ^[2]; Reiter, Robert E. ^[2]; Wu, Anna M. ^[2]

Univ. of California, Los Angeles, CA (United States); DOE Office of Scientific and Technical Information (OSTI)
Univ. of California, Los Angeles, CA (United States)

Here, prostate stem cell antigen (PSCA) is highly expressed in local prostate cancers and prostate cancer bone metastases and its expression correlates with androgen receptor activation and a poor prognosis. Here in this study, we investigate the potential clinical applications of immunoPET with the anti-PSCA A11 minibody, an antibody fragment optimized for use as an imaging agent. We compare A11 minibody immunoPET to ¹⁸F-Fluoride PET bone scans for detecting prostate cancer bone tumors and evaluate the ability of the A11 minibody to image tumor response to androgen deprivation. Osteoblastic, PSCA expressing, LAPC-9 intratibial xenografts were imaged with serial ¹²⁴I-anti-PSCA A11 minibody immunoPET and ¹⁸F-Fluoride bone scans. Mice bearing LAPC-9 subcutaneous xenografts were treated with either vehicle or MDV-3100 and imaged with A11 minibody immunoPET/CT scans pre- and post-treatment. Ex vivo flow cytometry measured the change in PSCA expression in response to androgen deprivation. A11 minibody demonstrated improved sensitivity and specificity over ¹⁸F-Fluoride bone scans for detecting LAPC-9 intratibial xenografts at all time points. Finally, LAPC-9 subcutaneous xenografts showed downregulation of PSCA when treated with MDV-3100 which A11 minibody immunoPET was able to detect in vivo.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of California, Los Angeles, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0001220

OSTI ID:: 1345504

Journal Information:: Clinical Cancer Research, Journal Name: Clinical Cancer Research Journal Issue: 24 Vol. 20; ISSN 1078-0432

Publisher:: American Association for Cancer ResearchCopyright Statement

Country of Publication:: United States

Language:: English

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Dual-Modality ImmunoPET/Fluorescence Imaging of Prostate Cancer with an Anti-PSCA Cys-Minibody Tsai, Wenting K.; Zettlitz, Kirstin A.; Tavaré, Richard Theranostics, Vol. 8, Issue 21 https://doi.org/10.7150/thno.27679	journal	January 2018
Bio‐Conjugated Advanced Materials for Targeted Disease Theranostics Hu, Xi‐Le; Kwon, Nahyun; Yan, Kai‐Cheng Advanced Functional Materials, Vol. 30, Issue 13 https://doi.org/10.1002/adfm.201907906	journal	March 2020
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Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
MDV-3100 (enzalutamide)
Prostate Stem Cell Antigen (PSCA)
immunoPET
minibody
prostate cancer bone metastases