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Title: Model Lung Surfactant Films: Why Composition Matters

Journal Article · · Langmuir
 [1];  [1];  [1];  [1]
  1. Concordia Univ., Montreal (Canada)
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Lung surfactant replacement therapies, Survanta and Infasurf, and two lipid-only systems both containing saturated and unsaturated phospholipids and one containing additional palmitic acid were used to study the impact of buffered saline on the surface activity, morphology, rheology, and structure of Langmuir monolayer model membranes. Isotherms and Brewster angle microscopy show that buffered saline subphases induce a film expansion, except when the cationic protein, SP-B, is present in sufficient quantities to already screen electrostatic repulsion, thus limiting the effect of changing pH and adding counterions. Grazing incidence X-ray diffraction results indicate an expansion not only of the liquid expanded phase but also an expansion of the lattice of the condensed phase. The film expansion corresponded in all cases with a significant reduction in the viscosity and elasticity of the films. The viscoelastic parameters are dominated by liquid expanded phase properties and do not appear to be dependent on the structure of the condensed phase domains in a phase separated film. Here, the results highlight that the choice of subphase and film composition is important for meaningful interpretations of measurements using model systems.

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Organization:
USDOE Office of Science (SC); Natural Science and Engineering Research Council; Canada Foundation for Innovation
Grant/Contract Number:
AC02-06CH11357; 03977-2014; 8071
OSTI ID:
1345413
Journal Information:
Langmuir, Vol. 32, Issue 41; ISSN 0743-7463
Publisher:
American Chemical SocietyCopyright Statement
Country of Publication:
United States
Language:
ENGLISH
Citation Metrics:
Cited by: 11 works
Citation information provided by
Web of Science

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Nanoparticle-induced structural changes in lung surfactant membranes: an X-ray scattering study journal January 2018
Physicochemical studies of direct interactions between lung surfactant and components of electronic cigarettes liquid mixtures journal April 2018
Physicochemical studies of direct interactions between lung surfactant and components of electronic cigarettes liquid mixtures text January 2018
Preparation of Non-Surface-Active Langmuir Trough Subphases from Milk journal September 2019

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36 MATERIALS SCIENCE
lipids
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phase transitions
surfactants