A sweet new role for LCP enzymes in protein glycosylation

Amer, Brendan R.; Clubb, Robert T.

doi:10.1111/mmi.12825

Title: A sweet new role for LCP enzymes in protein glycosylation

Journal Article · Fri Nov 21 00:00:00 EST 2014 · Molecular Microbiology

DOI:https://doi.org/10.1111/mmi.12825· OSTI ID:1344913

Amer, Brendan R. ^[1]; Clubb, Robert T. ^[1]

Univ. of California, Los Angeles, CA (United States)

Summary The peptidoglycan that surrounds G ram‐positive bacteria is affixed with a range of macromolecules that enable the microbe to effectively interact with its environment. Distinct enzymes decorate the cell wall with proteins and glycopolymers. Sortase enzymes covalently attach proteins to the peptidoglycan, while LytR ‐ CpsA ‐ Psr ( LCP ) proteins are thought to attach teichoic acid polymers and capsular polysaccharides. Ton‐That and colleagues have discovered a new glycosylation pathway in the oral bacterium A ctinomyces oris in which sortase and LCP enzymes operate on the same protein substrate. The A . oris LCP protein has a novel function, acting on the cell surface to transfer glycan macromolecules to a protein, which is then attached to the cell wall by a sortase. The reactions are tightly coupled, as elimination of the sortase causes the lethal accumulation of glycosylated protein in the membrane. Since sortase enzymes are attractive drug targets, this novel finding may provide a convenient cell‐based tool to discover inhibitors of this important enzyme family.

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Research Organization:: Univ. of California, Los Angeles, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: FC03-87ER60615; DE‐FC‐03‐87ER60615

OSTI ID:: 1344913

Alternate ID(s):: OSTI ID: 1400667

Journal Information:: Molecular Microbiology, Vol. 94, Issue 6; ISSN 0950-382X

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 6 works

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Cited By (2)

Interacting proteins of the essential two-component system YycFG in Bacillus subtilis Wu, Xuanang; Song, Qi; Han, Aidong Journal of Basic Microbiology, Vol. 59, Issue 9 https://doi.org/10.1002/jobm.201800701	journal	July 2019
Enterococcus hirae LcpA (Psr), a new peptidoglycan-binding protein localized at the division site Maréchal, Maxime; Amoroso, Ana; Morlot, Cécile BMC Microbiology, Vol. 16, Issue 1 https://doi.org/10.1186/s12866-016-0844-y	journal	October 2016

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