Alu repeats: Tools for mapping the human genome
- Lawrence Livermore National Lab., CA (United States); and others
There are over 500,000 Alu repeats dispersed throughout the human genome in a semi-random manner. Alu elements serve as priming sites for the amplification of unique DNA sequences located between Alu repeats that reside in the relatively close proximity in a process termed inter-Alu PCR. Physical mapping of the human genome involves a variety of complex hybridization based procedures. Some of these procedures rely upon the ability to separate human clones derived from human/rodent hybrid cell lines from those that contain background rodent derived DNA sequences. The ability to block the repetitive element (Alu repeat) portion of inter-Alu PCR products derived from a variety of complex sources is also crucial for the isolation of unique DNA sequences. We have constructed a new consensus Alu repeat probe (pPD39) designed for these purposes. We have also employed inter-Alu repeat PCR for the rapid detection of overlap between a number of chromosome 19-specific large insert bacterial and P1-derived artificial chromosomes (BACs and PACs) isolated from the total genomic libraries as well as the generation of human-specific probes to identify overlapping cosmid clones. Using this approach we have generated a high resolution BAC, PAC, and cosmid contig spanning over 440 kb of chromosome 19q13.2 containing the human DNA repair gene XRCC1. The overlap between the BAC, PAC and cosmid clones from the contig has been confirmed using automated EcoRI restriction site mapping.
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 134380
- Report Number(s):
- CONF-941009--
- Journal Information:
- American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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