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Title: Monitoring genetic and metabolic potential for in situ bioremediation: Mass spectrometry. 1997 annual progress report

Abstract

'A number of US Department of Energy (DOE) sites are contaminated with mixtures of dense non-aqueous phase liquids (DNAPLs) such as carbon tetrachloride, chloroform,. perchloroethylene, and trichloroethylene. At many of these sites, in situ microbial bioremediation is an attractive strategy for cleanup because it has the potential to degrade DNAPLs in situ without producing toxic byproducts. A rapid screening method to determine the broad range metabolic and genetic potential for contaminant degradation would greatly reduce the cost and time involved in assessment for in situ bioremediation as well as for monitoring ongoing bioremediation treatment. In this project, the ORNL Organic Mass Spectrometry (OMS) group is developing mass-spectrometry-based methods to screen for the genetic and metabolic potential for assessment and monitoring of in situ bioremediation of DNAPLs. In close collaboration, Professor Mary Lidstrom''s group at the University of Washington is identifying short DNA sequences related to microbial processes involved in the biodegradation of pollutants. This work will lay the foundation for development of a field-portable mass-spectrometry-based technique for rapid assessment and monitoring of bioremediation processes on site.'

Authors:
; ; ; ;
Publication Date:
Research Org.:
Oak Ridge National Lab., TN (US)
Sponsoring Org.:
USDOE Office of Environmental Management (EM), Office of Science and Risk Policy
OSTI Identifier:
13438
Report Number(s):
EMSP-55108-97
ON: DE00013438
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
44; 55; 54; Progress Report; Measuring Instruments; Medicine; Microorganisms; Genetics; Remedial Action; PROGRESS REPORT; MEASURING INSTRUMENTS; MEDICINE; MICROORGANISMS; GENETICS; REMEDIAL ACTION

Citation Formats

Buchanan, M.V., Hurst, G.B., Britt, P.F., McLuckey, S.A., and Doktycz, M.J. Monitoring genetic and metabolic potential for in situ bioremediation: Mass spectrometry. 1997 annual progress report. United States: N. p., 1997. Web. doi:10.2172/13438.
Buchanan, M.V., Hurst, G.B., Britt, P.F., McLuckey, S.A., & Doktycz, M.J. Monitoring genetic and metabolic potential for in situ bioremediation: Mass spectrometry. 1997 annual progress report. United States. doi:10.2172/13438.
Buchanan, M.V., Hurst, G.B., Britt, P.F., McLuckey, S.A., and Doktycz, M.J. Mon . "Monitoring genetic and metabolic potential for in situ bioremediation: Mass spectrometry. 1997 annual progress report". United States. doi:10.2172/13438. https://www.osti.gov/servlets/purl/13438.
@article{osti_13438,
title = {Monitoring genetic and metabolic potential for in situ bioremediation: Mass spectrometry. 1997 annual progress report},
author = {Buchanan, M.V. and Hurst, G.B. and Britt, P.F. and McLuckey, S.A. and Doktycz, M.J.},
abstractNote = {'A number of US Department of Energy (DOE) sites are contaminated with mixtures of dense non-aqueous phase liquids (DNAPLs) such as carbon tetrachloride, chloroform,. perchloroethylene, and trichloroethylene. At many of these sites, in situ microbial bioremediation is an attractive strategy for cleanup because it has the potential to degrade DNAPLs in situ without producing toxic byproducts. A rapid screening method to determine the broad range metabolic and genetic potential for contaminant degradation would greatly reduce the cost and time involved in assessment for in situ bioremediation as well as for monitoring ongoing bioremediation treatment. In this project, the ORNL Organic Mass Spectrometry (OMS) group is developing mass-spectrometry-based methods to screen for the genetic and metabolic potential for assessment and monitoring of in situ bioremediation of DNAPLs. In close collaboration, Professor Mary Lidstrom''s group at the University of Washington is identifying short DNA sequences related to microbial processes involved in the biodegradation of pollutants. This work will lay the foundation for development of a field-portable mass-spectrometry-based technique for rapid assessment and monitoring of bioremediation processes on site.'},
doi = {10.2172/13438},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Sep 01 00:00:00 EDT 1997},
month = {Mon Sep 01 00:00:00 EDT 1997}
}

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