Photocaged DNAzymes as a general method for sensing metal ions in living cells

Hwang, Kevin; Wu, Peiwen; Kim, Taejin; Lei, Lei; Tian, Shiliang; Wang, Yingxiao; Lu, Yi

doi:10.1002/anie.201408333

Photocaged DNAzymes as a general method for sensing metal ions in living cells

Journal Article · Tue Oct 14 00:00:00 EDT 2014 · Angewandte Chemie (International Edition)

DOI:https://doi.org/10.1002/anie.201408333· OSTI ID:1342732

Hwang, Kevin ^[1]; Wu, Peiwen ^[2]; Kim, Taejin ^[2]; Lei, Lei ^[3]; Tian, Shiliang ^[2]; Wang, Yingxiao ^[3]; Lu, Yi ^[2]

Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States); Office of Scientific and Technical Information (OSTI)
Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
Univ. of California, San Diego, La Jolla, CA (United States)

DNAzymes, which are sequences of DNA with catalytic activity, have been demonstrated as a potential platform for sensing a wide range of metal ions. Despite their significant promise, cellular sensing using DNAzymes has however been difficult, mainly because of the “always-on” mode of first-generation DNAzyme sensors. In this paper, to overcome this limitation, a photoactivatable (or photocaged) DNAzyme was designed and synthesized, and its application in sensing Zn^lI in living cells was demonstrated. In this design, the adenosine ribonucleotide at the scissile position of the 8–17 DNAzyme was replaced by 2'-O-nitrobenzyl adenosine, rendering the DNAzyme inactive and thus allowing its delivery into cells intact, protected from nonspecific degradation within cells. Irradiation at 365 nm restored DNAzyme activity, thus allowing the temporal control over the sensing activity of the DNAzyme for metal ions. Finally, the same strategy was also applied to the GR-5 DNAzyme for the detection of PbII, thus demonstrating the possible scope of the method.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Illinois, Urbana, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: FG02-08ER64568

OSTI ID:: 1342732

Journal Information:: Angewandte Chemie (International Edition), Journal Name: Angewandte Chemie (International Edition) Journal Issue: 50 Vol. 53; ISSN 1433-7851

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Photocaged DNAzymes as a general method for sensing metal ions in living cells

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