Photocaged DNAzymes as a general method for sensing metal ions in living cells

Hwang, Kevin; Wu, Peiwen; Kim, Taejin; Lei, Lei; Tian, Shiliang; Wang, Yingxiao; Lu, Yi

doi:10.1002/anie.201408333

Title: Photocaged DNAzymes as a general method for sensing metal ions in living cells

Journal Article · Tue Oct 14 00:00:00 EDT 2014 · Angewandte Chemie (International Edition)

DOI:https://doi.org/10.1002/anie.201408333· OSTI ID:1342732

Hwang, Kevin ^[1]; Wu, Peiwen ^[1]; Kim, Taejin ^[1]; Lei, Lei ^[2]; Tian, Shiliang ^[1]; Wang, Yingxiao ^[2]; Lu, Yi ^[1]

Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
Univ. of California, San Diego, La Jolla, CA (United States)

DNAzymes, which are sequences of DNA with catalytic activity, have been demonstrated as a potential platform for sensing a wide range of metal ions. Despite their significant promise, cellular sensing using DNAzymes has however been difficult, mainly because of the “always-on” mode of first-generation DNAzyme sensors. In this paper, to overcome this limitation, a photoactivatable (or photocaged) DNAzyme was designed and synthesized, and its application in sensing Zn^lI in living cells was demonstrated. In this design, the adenosine ribonucleotide at the scissile position of the 8–17 DNAzyme was replaced by 2'-O-nitrobenzyl adenosine, rendering the DNAzyme inactive and thus allowing its delivery into cells intact, protected from nonspecific degradation within cells. Irradiation at 365 nm restored DNAzyme activity, thus allowing the temporal control over the sensing activity of the DNAzyme for metal ions. Finally, the same strategy was also applied to the GR-5 DNAzyme for the detection of PbII, thus demonstrating the possible scope of the method.

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Research Organization:: Univ. of Illinois at Urbana-Champaign, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: FG02-08ER64568

OSTI ID:: 1342732

Journal Information:: Angewandte Chemie (International Edition), Vol. 53, Issue 50; ISSN 1433-7851

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 128 works

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References (70)

Zinc homeostasis and signaling in health and diseases: Zinc signaling Fukada, Toshiyuki; Yamasaki, Satoru; Nishida, Keigo JBIC Journal of Biological Inorganic Chemistry, Vol. 16, Issue 7 https://doi.org/10.1007/s00775-011-0797-4	journal	June 2011
Calcium Signaling Clapham, David E. Cell, Vol. 131, Issue 6 https://doi.org/10.1016/j.cell.2007.11.028	journal	December 2007
Femtomolar Sensitivity of Metalloregulatory Proteins Controlling Zinc Homeostasis Outten, C. E. Science, Vol. 292, Issue 5526 https://doi.org/10.1126/science.1060331	journal	June 2001
The tightly regulated copper window in yeast Wegner, Seraphine V.; Sun, Fei; Hernandez, Nick Chem. Commun., Vol. 47, Issue 9 https://doi.org/10.1039/C0CC04292G	journal	January 2011
Fluorescent detection of zinc in biological systems: recent development on the design of chemosensors and biosensors Jiang, Pengju; Guo, Zijian Coordination Chemistry Reviews, Vol. 248, Issue 1-2 https://doi.org/10.1016/j.cct.2003.10.013	journal	January 2004
Identifying metalloproteins through X-ray fluorescence mapping and mass spectrometry Raimunda, Daniel; Khare, Tripti; Giometti, Carol Metallomics, Vol. 4, Issue 9 https://doi.org/10.1039/c2mt20095c	journal	January 2012
Photochemical Tools for Studying Metal Ion Signaling and Homeostasis Mbatia, Hannah W.; Burdette, Shawn C. Biochemistry, Vol. 51, Issue 37 https://doi.org/10.1021/bi3001769	journal	September 2012
Continuing Issues with Lead: Recent Advances in Detection Deibler, Kristine; Basu, Partha European Journal of Inorganic Chemistry, Vol. 2013, Issue 7 https://doi.org/10.1002/ejic.201200997	journal	November 2012
In Situ Imaging of Metals in Cells and Tissues McRae, Reagan; Bagchi, Pritha; Sumalekshmy, S. Chemical Reviews, Vol. 109, Issue 10 https://doi.org/10.1021/cr900223a	journal	October 2009
Zinc sensing for cellular application Kikuchi, Kazuya; Komatsu, Kensuke; Nagano, Tetsuo Current Opinion in Chemical Biology, Vol. 8, Issue 2 https://doi.org/10.1016/j.cbpa.2004.02.007	journal	April 2004
Synthetic fluorescent sensors for studying the cell biology of metals Domaille, Dylan W.; Que, Emily L.; Chang, Christopher J. Nature Chemical Biology, Vol. 4, Issue 3 https://doi.org/10.1038/nchembio.69	journal	February 2008
Metals in Neurobiology: Probing Their Chemistry and Biology with Molecular Imaging Que, Emily L.; Domaille, Dylan W.; Chang, Christopher J. Chemical Reviews, Vol. 108, Issue 5 https://doi.org/10.1021/cr078203u	journal	May 2008
Imaging mobile zinc in biology Tomat, Elisa; Lippard, Stephen J. Current Opinion in Chemical Biology, Vol. 14, Issue 2 https://doi.org/10.1016/j.cbpa.2009.12.010	journal	April 2010
Biochemistry of Mobile Zinc and Nitric Oxide Revealed by Fluorescent Sensors Pluth, Michael D.; Tomat, Elisa; Lippard, Stephen J. Annual Review of Biochemistry, Vol. 80, Issue 1 https://doi.org/10.1146/annurev-biochem-061009-091643	journal	July 2011
Visualizing metal ions in cells: An overview of analytical techniques, approaches, and probes Dean, Kevin M.; Qin, Yan; Palmer, Amy E. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol. 1823, Issue 9 https://doi.org/10.1016/j.bbamcr.2012.04.001	journal	September 2012
Genetically Encoded Copper(I) Reporters with Improved Response for Use in Imaging Liu, Jun; Karpus, Jason; Wegner, Seraphine V. Journal of the American Chemical Society, Vol. 135, Issue 8 https://doi.org/10.1021/ja3106779	journal	February 2013
Strategies in detection of metal ions using dyes Sareen, Divya; Kaur, Paramjit; Singh, Kamaljit Coordination Chemistry Reviews, Vol. 265 https://doi.org/10.1016/j.ccr.2014.01.015	journal	April 2014
Synthetic fluorescent probes for monovalent copper Fahrni, Christoph J. Current Opinion in Chemical Biology, Vol. 17, Issue 4 https://doi.org/10.1016/j.cbpa.2013.05.019	journal	August 2013
Selective Zinc Sensor Molecules with Various Affinities for Zn ²⁺ , Revealing Dynamics and Regional Distribution of Synaptically Released Zn ²⁺ in Hippocampal Slices Komatsu, Kensuke; Kikuchi, Kazuya; Kojima, Hirotatsu Journal of the American Chemical Society, Vol. 127, Issue 29 https://doi.org/10.1021/ja050301e	journal	July 2005
Functional Nucleic Acid Sensors Liu, Juewen; Cao, Zehui; Lu, Yi Chemical Reviews, Vol. 109, Issue 5 https://doi.org/10.1021/cr030183i	journal	May 2009
DNA as Sensors and Imaging Agents for Metal Ions Xiang, Yu; Lu, Yi Inorganic Chemistry, Vol. 53, Issue 4 https://doi.org/10.1021/ic4019103	journal	December 2013
Directed Evolution of Nucleic Acid Enzymes Joyce, Gerald F. Annual Review of Biochemistry, Vol. 73, Issue 1 https://doi.org/10.1146/annurev.biochem.73.011303.073717	journal	June 2004
In Vitro Selection of High Temperature Zn2+-Dependent DNAzymes Nelson, Kevin E.; Bruesehoff, Peter J.; Lu, Yi Journal of Molecular Evolution, Vol. 61, Issue 2 https://doi.org/10.1007/s00239-004-0374-3	journal	August 2005
A DNA enzyme that cleaves RNA Breaker, Ronald R.; Joyce, Gerald F. Chemistry & Biology, Vol. 1, Issue 4, p. 223-229 https://doi.org/10.1016/1074-5521(94)90014-0	journal	December 1994
Functional Nucleic Acids as Molecular Recognition Elements for Small Organic and Biological Molecules Sai Lau, Pui; Li, Yingfu Current Organic Chemistry, Vol. 15, Issue 4 https://doi.org/10.2174/138527211794474537	journal	February 2011
Deoxyribozymes: New players in the ancient game of biocatalysis Li, Yingfu; Breaker, Ronald R. Current Opinion in Structural Biology, Vol. 9, Issue 3 https://doi.org/10.1016/S0959-440X(99)80042-6	journal	June 1999
A catalytic DNA for porphyrin metallation Li, Yingfu; Sen, Dipankar Nature Structural & Molecular Biology, Vol. 3, Issue 9 https://doi.org/10.1038/nsb0996-743	journal	September 1996
Metal Ion Sensors Based on DNAzymes and Related DNA Molecules Zhang, Xiao-Bing; Kong, Rong-Mei; Lu, Yi Annual Review of Analytical Chemistry, Vol. 4, Issue 1 https://doi.org/10.1146/annurev.anchem.111808.073617	journal	July 2011
Label-Free Colorimetric Detection of Aqueous Mercury Ion (Hg ²⁺ ) Using Hg ²⁺ -Modulated G-Quadruplex-Based DNAzymes Li, Tao; Dong, Shaojun; Wang, Erkang Analytical Chemistry, Vol. 81, Issue 6 https://doi.org/10.1021/ac900188y	journal	March 2009
DNAzyme-based colorimetric sensing of lead (Pb ²⁺ ) using unmodified gold nanoparticle probes Wei, Hui; Li, Bingling; Li, Jing Nanotechnology, Vol. 19, Issue 9 https://doi.org/10.1088/0957-4484/19/9/095501	journal	February 2008
Catalytic and Molecular Beacons for Amplified Detection of Metal Ions and Organic Molecules with High Sensitivity Zhang, Xiao-Bing; Wang, Zidong; Xing, Hang Analytical Chemistry, Vol. 82, Issue 12 https://doi.org/10.1021/ac1009047	journal	June 2010
In vitro selection and characterization of a highly efficient Zn(II)-dependent RNA-cleaving deoxyribozyme Li, J. Nucleic Acids Research, Vol. 28, Issue 2 https://doi.org/10.1093/nar/28.2.481	journal	January 2000
Improving Fluorescent DNAzyme Biosensors by Combining Inter- and Intramolecular Quenchers Liu, Juewen; Lu, Yi Analytical Chemistry, Vol. 75, Issue 23 https://doi.org/10.1021/ac034924r	journal	December 2003
A Highly Selective DNAzyme Sensor for Mercuric Ions Hollenstein, Marcel; Hipolito, Christopher; Lam, Curtis Angewandte Chemie International Edition, Vol. 47, Issue 23 https://doi.org/10.1002/anie.200800960	journal	May 2008
A Highly Selective DNAzyme Sensor for Mercuric Ions Hollenstein, Marcel; Hipolito, Christopher; Lam, Curtis Angewandte Chemie, Vol. 120, Issue 23 https://doi.org/10.1002/ange.200800960	journal	May 2008
Accelerated Color Change of Gold Nanoparticles Assembled by DNAzymes for Simple and Fast Colorimetric Pb ²⁺ Detection Liu, Juewen; Lu, Yi Journal of the American Chemical Society, Vol. 126, Issue 39 https://doi.org/10.1021/ja046628h	journal	October 2004
Stimuli-Responsive Disassembly of Nanoparticle Aggregates for Light-Up Colorimetric Sensing Liu, Juewen; Lu, Yi Journal of the American Chemical Society, Vol. 127, Issue 36, p. 12677-12683 https://doi.org/10.1021/ja053567u	journal	September 2005
Using personal glucose meters and functional DNA sensors to quantify a variety of analytical targets Xiang, Yu; Lu, Yi Nature Chemistry, Vol. 3, Issue 9 https://doi.org/10.1038/nchem.1092	journal	July 2011
A DNAzyme-Gold Nanoparticle Probe for Uranyl Ion in Living Cells Wu, Peiwen; Hwang, Kevin; Lan, Tian Journal of the American Chemical Society, Vol. 135, Issue 14 https://doi.org/10.1021/ja400150v	journal	March 2013
Functionalization of cationic poly(p-phenylene ethynylene) with dendritic polyethylene enables efficient DNAzyme delivery for imaging Pb ²⁺ in living cells Zhang, Ling; Huang, Hongying; Xu, Na Journal of Materials Chemistry B, Vol. 2, Issue 30 https://doi.org/10.1039/C4TB00680A	journal	January 2014
Caged RNA: photo-control of a ribozyme reaction Chaulk, S. Nucleic Acids Research, Vol. 26, Issue 13 https://doi.org/10.1093/nar/26.13.3173	journal	July 1998
Light-regulated Catalysis by an RNA-cleaving Deoxyribozyme Liu, Yong; Sen, Dipankar Journal of Molecular Biology, Vol. 341, Issue 4 https://doi.org/10.1016/j.jmb.2004.06.060	journal	August 2004
Photochemical DNA Activation Lusic, Hrvoje; Young, Douglas D.; Lively, Mark O. Organic Letters, Vol. 9, Issue 10 https://doi.org/10.1021/ol070455u	journal	May 2007
Turning the 10-23 DNAzyme On and Off with Light Richards, Julia L.; Seward, Garry K.; Wang, Yu-Hsiu ChemBioChem, Vol. 11, Issue 3 https://doi.org/10.1002/cbic.200900702	journal	February 2010
Triggering DNAzymes with Light: A Photoactive C8 Thioether-Linked Adenosine Ting, Richard; Lermer, Leonard; Perrin, David M. Journal of the American Chemical Society, Vol. 126, Issue 40 https://doi.org/10.1021/ja046964y	journal	October 2004
Activation and Deactivation of DNAzyme and Antisense Function with Light for the Photochemical Regulation of Gene Expression in Mammalian Cells Young, Douglas D.; Lively, Mark O.; Deiters, Alexander Journal of the American Chemical Society, Vol. 132, Issue 17 https://doi.org/10.1021/ja100710j	journal	May 2010
Reversible Photocontrol of Deoxyribozyme-Catalyzed RNA Cleavage under Multiple-Turnover Conditions Keiper, Sonja; Vyle, Joseph S. Angewandte Chemie International Edition, Vol. 45, Issue 20 https://doi.org/10.1002/anie.200600164	journal	May 2006
Reversible Photocontrol of Deoxyribozyme-Catalyzed RNA Cleavage under Multiple-Turnover Conditions Keiper, Sonja; Vyle, Joseph S. Angewandte Chemie, Vol. 118, Issue 20 https://doi.org/10.1002/ange.200600164	journal	May 2006
Characterization and development of photoactivatable fluorescent proteins for single-molecule-based superresolution imaging Wang, S.; Moffitt, J. R.; Dempsey, G. T. Proceedings of the National Academy of Sciences, Vol. 111, Issue 23 https://doi.org/10.1073/pnas.1406593111	journal	May 2014
Photoactivatable fluorescent proteins Lukyanov, Konstantin A.; Chudakov, Dmitry M.; Lukyanov, Sergey Nature Reviews Molecular Cell Biology, Vol. 6, Issue 11 https://doi.org/10.1038/nrm1741	journal	September 2005
Photoremovable protecting groups: reaction mechanisms and applications Pelliccioli, Anna Paola; Wirz, Jakob Photochemical & Photobiological Sciences, Vol. 1, Issue 7 https://doi.org/10.1039/b200777k	journal	June 2002
Photoactive molecules for applications in molecular imaging and cell biology Shao, Qing; Xing, Bengang Chemical Society Reviews, Vol. 39, Issue 8 https://doi.org/10.1039/b915574k	journal	January 2010
Optical Control of Protein Function through Unnatural Amino Acid Mutagenesis and Other Optogenetic Approaches Baker, Austin S.; Deiters, Alexander ACS Chemical Biology, Vol. 9, Issue 7 https://doi.org/10.1021/cb500176x	journal	May 2014
Photocontrol of Tyrosine Phosphorylation in Mammalian Cells via Genetic Encoding of Photocaged Tyrosine Arbely, Eyal; Torres-Kolbus, Jessica; Deiters, Alexander Journal of the American Chemical Society, Vol. 134, Issue 29 https://doi.org/10.1021/ja3046958	journal	July 2012
Photocontrolled Compound Release System Using Caged Antimicrobial Peptide Mizukami, Shin; Hosoda, Mariko; Satake, Takafumi Journal of the American Chemical Society, Vol. 132, Issue 28 https://doi.org/10.1021/ja102167m	journal	July 2010
In Vitro and In Vivo Uncaging and Bioluminescence Imaging by Using Photocaged Upconversion Nanoparticles Yang, Yanmei; Shao, Qing; Deng, Renren Angewandte Chemie International Edition, Vol. 51, Issue 13 https://doi.org/10.1002/anie.201107919	journal	January 2012
In Vitro and In Vivo Uncaging and Bioluminescence Imaging by Using Photocaged Upconversion Nanoparticles Yang, Yanmei; Shao, Qing; Deng, Renren Angewandte Chemie, Vol. 124, Issue 13 https://doi.org/10.1002/ange.201107919	journal	January 2012
Photoactivatable RNA derivatives as tools for studying the structural and functional organization of complex cellular ribonucleoprotein machineries Graifer, Dmitri; Karpova, Galina RSC Adv., Vol. 3, Issue 9 https://doi.org/10.1039/C2RA22095D	journal	January 2013
Targeting Expression with Light Using Caged DNA Monroe, W. Todd; McQuain, Mark M.; Chang, Min S. Journal of Biological Chemistry, Vol. 274, Issue 30 https://doi.org/10.1074/jbc.274.30.20895	journal	July 1999
Light-activated gene expression from site-specific caged DNA with a biotinylated photolabile protection group Yamaguchi, Satoshi; Chen, Yanjie; Nakajima, Soh Chemical Communications, Vol. 46, Issue 13 https://doi.org/10.1039/b922502a	journal	January 2010
Intracellular Light-Activation of Riboswitch Activity Walsh, Steven; Gardner, Laura; Deiters, Alexander ChemBioChem, Vol. 15, Issue 9 https://doi.org/10.1002/cbic.201400024	journal	May 2014
Control of Oncogenic miRNA Function by Light-Activated miRNA Antagomirs Connelly, Colleen M.; Deiters, Alexander Methods in Molecular Biology https://doi.org/10.1007/978-1-4939-0856-1_9	book	January 2014
Sequential Gene Silencing Using Wavelength-Selective Caged Morpholino Oligonucleotides Yamazoe, Sayumi; Liu, Qingyang; McQuade, Lindsey E. Angewandte Chemie International Edition, Vol. 53, Issue 38 https://doi.org/10.1002/anie.201405355	journal	August 2014
Sequential Gene Silencing Using Wavelength-Selective Caged Morpholino Oligonucleotides Yamazoe, Sayumi; Liu, Qingyang; McQuade, Lindsey E. Angewandte Chemie, Vol. 126, Issue 38 https://doi.org/10.1002/ange.201405355	journal	August 2014
Quantitative Three-Dimensional Analysis of the Intracellular Trafficking of Plasmid DNA Transfected by a Nonviral Gene Delivery System Using Confocal Laser Scanning Microscopy Akita, H.; Ito, R.; Khalil, I. A. Molecular Therapy, Vol. 9, Issue 3 https://doi.org/10.1016/j.ymthe.2004.01.005	journal	March 2004
A Versatile Endoribonuclease Mimic Made of DNA: Characteristics and Applications of the 8-17 RNA-Cleaving DNAzyme Schlosser , Kenny; Li, Yingfu ChemBioChem, Vol. 11, Issue 7 https://doi.org/10.1002/cbic.200900786	journal	March 2010
A highly selective lead sensor based on a classic lead DNAzyme Lan, Tian; Furuya, Kimberly; Lu, Yi Chemical Communications, Vol. 46, Issue 22 https://doi.org/10.1039/b926910j	journal	January 2010
Continuing Issues with Lead: Recent Advances in Detection Deibler, Kristine; Basu, Partha European Journal of Inorganic Chemistry, Vol. 2013, Issue 7 https://doi.org/10.1002/ejic.201300140	journal	February 2013
Calcium Signaling Bootman, M. D. Cold Spring Harbor Perspectives in Biology, Vol. 4, Issue 7 https://doi.org/10.1101/cshperspect.a011171	journal	July 2012
Calcium signaling Clapham, David E. Cell, Vol. 80, Issue 2 https://doi.org/10.1016/0092-8674(95)90408-5	journal	January 1995

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fluorescent probes
photolabile protecting groups

Title: Photocaged DNAzymes as a general method for sensing metal ions in living cells

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