Imaging of Hydrogel Microsphere Structure and Foreign Body Response Based on Endogenous X-Ray Phase Contrast

Appel, Alyssa A.; Ibarra, Veronica; Somo, Sami I.; Larson, Jeffery C.; Garson, Alfred B.; Guan, Huifeng; McQuilling, John Patrick; Zhong, Zhong; Anastasio, Mark A.; Opara, Emmanuel C.; Brey, Eric M.

doi:10.1089/ten.tec.2016.0253

Imaging of Hydrogel Microsphere Structure and Foreign Body Response Based on Endogenous X-Ray Phase Contrast

Journal Article · Mon Oct 31 00:00:00 EDT 2016 · Tissue Engineering. Part C: Methods

DOI:https://doi.org/10.1089/ten.tec.2016.0253· OSTI ID:1341702

Appel, Alyssa A. ^[1]; Ibarra, Veronica ^[2]; Somo, Sami I. ^[2]; Larson, Jeffery C. ^[1]; Garson, Alfred B. ^[3]; Guan, Huifeng ^[3]; McQuilling, John Patrick ^[4]; Zhong, Zhong ^[5]; Anastasio, Mark A. ^[3]; Opara, Emmanuel C. ^[4]; Brey, Eric M. ^[1]

Illinois Inst. of Technology, Chicago, IL (United States). Dept. of Biomedical Engineering; Edward Hines Jr. VA Hospital, Chicago, IL (United States)
Illinois Inst. of Technology, Chicago, IL (United States). Dept. of Biomedical Engineering
Washington Univ., St. Louis, MO (United States). Dept. of Biomedical Engineering
Wake Forest Inst. of Regenerative Medicine, Winston-Salem, NC (United States)
Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source

Transplantation of functional islets encapsulated in stable biomaterials has the potential to cure Type I diabetes. However, the success of these materials requires the ability to understand their stability in vivo. Imaging techniques that enable monitoring of biomaterial performance are critical to further development in the field. In this study, we demonstrate for the first time that X-ray phase contrast (XPC) imaging techniques enable 3D imaging and evaluation of islet volume, alginate hydrogel structure and local soft tissue response. Islets were encapsulated in alginate systems prepared in methods used in clinical trials and implanted in a rodent omentum pouch model as a treatment for type I diabetes. Microbeads were imaged with XPC prior to implantation and following implantation into an omentum pouch. Islets could be identified within alginate beads and the islet volume quantified. Omental adipose tissue could be distinguished from inflammatory regions resulting from implanted beads. Individual beads and the local encapsulation response were visualized and quantifiable. Measurements were in agreement with histology. The 3D structure of the microbeads could be characterized with XPC and failed beads could also be identified. These results point to the substantial potential of XPC as a tool for imaging biomaterials in small animal models.

View Accepted Manuscript (DOE)

Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

OSTI ID:: 1341702

Report Number(s):: BNL--113449-2017-JA

Journal Information:: Tissue Engineering. Part C: Methods, Journal Name: Tissue Engineering. Part C: Methods Journal Issue: 11 Vol. 22; ISSN 1937-3384

Publisher:: Mary Ann Liebert, Inc.Copyright Statement

Country of Publication:: United States

Language:: English

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