The past, present and future of microbiome analyses

White, Richard Allen; Callister, Stephen J.; Moore, Ronald J.; Baker, Erin S.; Jansson, Janet K.

doi:10.1038/nprot.2016.148

The past, present and future of microbiome analyses

Journal Article · Thu Sep 29 00:00:00 EDT 2016 · Nature Protocols

DOI:https://doi.org/10.1038/nprot.2016.148· OSTI ID:1340873

White, Richard Allen; Callister, Stephen J.; Moore, Ronald J.; Baker, Erin S.; Jansson, Janet K.

Microbes evolved on Earth approximately 3.5 billion years ago and eventually occupied every habitable environment in its biosphere. While microorganisms are responsible for key functions on Earth including carbon and nutrient cycling and determining the health and disease of its inhabitants (i.e. plants and animals), >99% of the estimated trillions of microbes have yet to be discovered.1 In addition, high microbial diversity has made it difficult to study specific functions carried out by complex microbial communities within "microbiomes", such as those inhabiting the soil and human gut.2,3 Fortunately, technological advances over the last few decades have greatly facilitated studies of complex microbiomes and their functions. Here we will specifically discuss advances related to nucleic acid sequencing and mass spectrometry analyses that have enabled exploration and understanding of complex microbiomes in a range of environments and our own bodies.4,5,6,7

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (US)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1340873

Report Number(s):: PNNL-SA-119986

Journal Information:: Nature Protocols, Journal Name: Nature Protocols Journal Issue: 11 Vol. 11; ISSN 1754-2189

Publisher:: Nature Publishing Group

Country of Publication:: United States

Language:: English

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