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Predictive Capabilities of in Vitro Assays for Estimating Pb Relative Bioavailability in Phosphate Amended Soils

Journal Article · · Environmental Science and Technology
 [1];  [2];  [3];  [1]
  1. Univ. of South Australia (Australia)
  2. U.S. Environmental Protection Agency, Cincinnati, OH (United States)
  3. U.S. Environmental Protection Agency, Cincinnati, OH (United States); Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)
+ Show Author Affiliations
In this study, the in vitro bioaccessibility (IVBA) of lead (Pb) in phosphate-amended Pb-contaminated soil was assessed using a variety of IVBA assays with an overarching aim of determining whether changes in Pb IVBA were congruent to those observed for Pb relative bioavailability (RBA) determined using an in vivo mouse assay. Here, amending soil with phosphoric acid or rock phosphate resulted in changes in Pb speciation, however, varying Pb IVBA results were obtained depending on the methodology utilized. In addition, IVBA assays influenced Pb speciation as a consequence of interactions between dissolved Pb and unreacted phosphate arising from the amendment or from assay constituents. When the relationship between Pb RBA and IVBA was assessed, a comparison of treatment effect ratios (Pb RBA or IVBA in treated soil divided by Pb RBA or IVBA for untreated soil) provided the best in vivo–in vitro correlation particular for SBRC (r2 = 0.83) and IVG (r2 = 0.89) intestinal extraction. For these assays, the slope of the lines of best fit were close to 1 (1.12, 0.82; SBRC, IVG intestinal extraction respectively) with small y-intercepts (0.09, 0.08 respectively) indicating that the efficacy of phosphate amendments for reducing Pb RBA may be predicted using IVBA assays.
Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
AC02-06CH11357
OSTI ID:
1340718
Journal Information:
Environmental Science and Technology, Journal Name: Environmental Science and Technology Journal Issue: 23 Vol. 50; ISSN 0013-936X
Publisher:
American Chemical Society (ACS)Copyright Statement
Country of Publication:
United States
Language:
ENGLISH

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Cited By (5)

Organic matter facilitates the binding of Pb to iron oxides in a subtropical contaminated soil journal September 2018
Urban soil and human health: a review: Urban soil and human health journal January 2018
Point of Zero Charge: Role in Pyromorphite Formation and Bioaccessibility of Lead and Arsenic in Phosphate-Amended Soils journal April 2018
Organic matter facilitates the binding of Pb to iron oxides in a subtropical contaminated soil text January 2018
Organic matter facilitates the binding of Pb to iron oxides in a subtropical contaminated soil text January 2018

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Related Subjects

54 ENVIRONMENTAL SCIENCES
Raman spectroscopy
assays
extraction
phosphates
soils