Multiplex automated analysis of microsatellite loci for linkage analysis of the entire human genome
- NIH, NIDR, MEDIB, Bethesda, MD (United States); and others
We are evaluating 29 panels of fluorescently labeled markers located at approximately 10 cM intervals. Each chromosome is covered at this marker density in 1-4 panels (11-17 loci/panel). Individual markers are labeled with 1 of 3 different fluorescent dyes, combined after PCR and run in a single gel lane. Genotypes are obtained for each locus using Applied Biosystems automated DNA sequencers and GENESCAN analysis and Genotyper allele scoring software. These programs automate the identification of alleles by distinguishing major peaks from PCR artifacts and facilitate export of data in a format suitable for standard genetic analysis programs. To verify the reported genetic relationships among individuals involved in gene mapping studies, we developed software to determine the number of alleles shared among individuals within a family. We use these statistics to distinguish full and half sibs and parent-child relations from unrelated individuals. Finally, we are developing a database using Fourth Dimension software so that the tremendous amounts of data generated can be processed efficiently in an integrated suite of specialized computer programs for linkage/association studies.
- OSTI ID:
- 134016
- Report Number(s):
- CONF-941009--
- Journal Information:
- American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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Automation of genetic linkage analysis using florescent microsatellite markers
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Related Subjects
INCLUDING NUCLEAR AND PARTICLE DETECTORS
55 BIOLOGY AND MEDICINE
BASIC STUDIES
99 GENERAL AND MISCELLANEOUS
AUTOMATION
COMPUTER CALCULATIONS
DATA PROCESSING
DNA SEQUENCING
EFFICIENCY
FLUORESCENCE
G CODES
GENES
GENETIC MAPPING
GENETICS
GENOTYPE
HUMAN CHROMOSOMES
POLYMERASE CHAIN REACTION