Multiplex automated analysis of microsatellite loci for linkage analysis of the entire human genome
- NIH, NIDR, MEDIB, Bethesda, MD (United States); and others
We are evaluating 29 panels of fluorescently labeled markers located at approximately 10 cM intervals. Each chromosome is covered at this marker density in 1-4 panels (11-17 loci/panel). Individual markers are labeled with 1 of 3 different fluorescent dyes, combined after PCR and run in a single gel lane. Genotypes are obtained for each locus using Applied Biosystems automated DNA sequencers and GENESCAN analysis and Genotyper allele scoring software. These programs automate the identification of alleles by distinguishing major peaks from PCR artifacts and facilitate export of data in a format suitable for standard genetic analysis programs. To verify the reported genetic relationships among individuals involved in gene mapping studies, we developed software to determine the number of alleles shared among individuals within a family. We use these statistics to distinguish full and half sibs and parent-child relations from unrelated individuals. Finally, we are developing a database using Fourth Dimension software so that the tremendous amounts of data generated can be processed efficiently in an integrated suite of specialized computer programs for linkage/association studies.
- OSTI ID:
- 134016
- Report Number(s):
- CONF-941009-; ISSN 0002-9297; TRN: 95:005313-0751
- Journal Information:
- American Journal of Human Genetics, Vol. 55, Issue Suppl.3; Conference: 44. annual meeting of the American Society of Human Genetics, Montreal (Canada), 18-22 Oct 1994; Other Information: PBD: Sep 1994
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
99 MATHEMATICS
COMPUTERS
INFORMATION SCIENCE
MANAGEMENT
LAW
MISCELLANEOUS
44 INSTRUMENTATION
INCLUDING NUCLEAR AND PARTICLE DETECTORS
GENES
GENETIC MAPPING
HUMAN CHROMOSOMES
AUTOMATION
G CODES
DATA PROCESSING
EFFICIENCY
GENETICS
COMPUTER CALCULATIONS
GENOTYPE
POLYMERASE CHAIN REACTION
DNA SEQUENCING
FLUORESCENCE