Bystander Effects of Ionizing Radiation
- Harvard T.H. Chan School of Public Health, Boston, MA (United States). Dept. of Genetics and Complex Diseases
The objectives of this grant renewal are to provide administrative support and travel funds to allow the continued participation of the principal investigator (Dr. John B. Little) as an advisor to research initiated by several research fellows from his laboratory. The actual research will be carried out under the direction of Dr. Hatsumi Nagasawa with the collaboration of Dr. Joel Bedford at the Colorado State University, and by Drs. Edouard Azzam and Sonia de Toledo at the University of Medicine and Dentistry of New Jersey. Dr. Little will advise on the planning of experiments and development of experimental protocols, the analysis of data, and the preparation of manuscripts for publication. The Specific Aims for several of the planned experiments include: 1) to extend studies of the role of recombinational repair in the bystander effect by examining other genes in this pathway and cell lines deficient in excision repair; 2) to continue studies to determine the nature of the damage signal transmitted to bystander cells including the expression of several connexins in the bystander response, and the extent to which the enhanced oxidative metabolism observed in bystander cells may relate to the nature of the transmitted bystander signal; 3) to utilize a genome-wide approach to examine the genetic basis for the hypersensitivity to ionization we have observed in unaffected parents of patients with hereditary retinoblastoma, as well as from a group of apparently normal individuals that show similar radiosensitivity; 4) to complete studies concerning the induction of high frequencies of cells with massive chromosome damage in clonal derivatives of p53 and p21 knockout mouse cell lines; in particular to examine the role of telomere changes in this phenomenon. Overall, the results of these studies should enhance our understanding of the risk of low-dose exposures to ionizing radiation, including human populations to residential radon as well as occupational exposures.
- Research Organization:
- Harvard T.H. Chan School of Public Health, Boston, MA (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- DOE Contract Number:
- FG02-05ER64089
- OSTI ID:
- 1339440
- Report Number(s):
- DOE-HSPH-64089; TRN: US1701472
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA REPAIR
BYSTANDER EFFECTS
IONIZING RADIATIONS
HUMAN POPULATIONS
MICE
RADON
DATA ANALYSIS
BIOLOGICAL RADIATION EFFECTS
OXIDATION
RADIOSENSITIVITY
SIGNALS
OCCUPATIONAL EXPOSURE
CHROMOSOMES
HAZARDS
IONIZATION
METABOLISM
TELOMERES
EXPERIMENT PLANNING
LOW DOSE IRRADIATION