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Title: Acridine Orange Indicates Early Oxidation of Wood Cell Walls by Fungi

Abstract

Colonization of wood blocks by brown and white rot fungi rapidly resulted in detectable wood oxidation, as shown by a reduced phloroglucinol response, a loss of autofluorescence, and acridine orange (AO) staining. This last approach is shown to provide a novel method for identifying wood oxidation. When lignin was mildly oxidized, the association between AO and lignin was reduced such that stained wood sections emitted less green light during fluorescence microscopy. This change was detectable after less than a week, an interval that past work has shown to be too short for significant delignification of wood. Although fungal hyphae were observed in only a few wood lumina, oxidation was widespread, appearing relatively uniform over regions several hundred micrometers from the hyphae. As a result, this observation suggests that both classes of fungi release low molecular weight mild oxidants during the first few days of colonization.

Authors:
 [1];  [2];  [3];  [4];  [1]
  1. USDA Forest Service, Madison, WI (United States). Forest Products Lab.
  2. Univ. of Wisconsin, Madison, WI (United States). Dept. of Bacteriology
  3. Univ. of Iowa, Iowa City, IA (United States). Dept. of Microbiology
  4. USDA Forest Service, Madison, WI (United States). Forest Products Lab.; Univ. of Wisconsin, Madison, WI (United States). Dept. of Bacteriology
Publication Date:
Research Org.:
USDA Forest Service, Madison, WI (United States). Forest Products Lab.
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1337469
Grant/Contract Number:
SC0006929; AI02-07ER64491
Resource Type:
Journal Article: Published Article
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 11; Journal Issue: 7; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Houtman, Carl J., Kitin, Peter, Houtman, Jon C. D., Hammel, Kenneth E., and Hunt, Christopher G. Acridine Orange Indicates Early Oxidation of Wood Cell Walls by Fungi. United States: N. p., 2016. Web. doi:10.1371/journal.pone.0159715.
Houtman, Carl J., Kitin, Peter, Houtman, Jon C. D., Hammel, Kenneth E., & Hunt, Christopher G. Acridine Orange Indicates Early Oxidation of Wood Cell Walls by Fungi. United States. doi:10.1371/journal.pone.0159715.
Houtman, Carl J., Kitin, Peter, Houtman, Jon C. D., Hammel, Kenneth E., and Hunt, Christopher G. 2016. "Acridine Orange Indicates Early Oxidation of Wood Cell Walls by Fungi". United States. doi:10.1371/journal.pone.0159715.
@article{osti_1337469,
title = {Acridine Orange Indicates Early Oxidation of Wood Cell Walls by Fungi},
author = {Houtman, Carl J. and Kitin, Peter and Houtman, Jon C. D. and Hammel, Kenneth E. and Hunt, Christopher G.},
abstractNote = {Colonization of wood blocks by brown and white rot fungi rapidly resulted in detectable wood oxidation, as shown by a reduced phloroglucinol response, a loss of autofluorescence, and acridine orange (AO) staining. This last approach is shown to provide a novel method for identifying wood oxidation. When lignin was mildly oxidized, the association between AO and lignin was reduced such that stained wood sections emitted less green light during fluorescence microscopy. This change was detectable after less than a week, an interval that past work has shown to be too short for significant delignification of wood. Although fungal hyphae were observed in only a few wood lumina, oxidation was widespread, appearing relatively uniform over regions several hundred micrometers from the hyphae. As a result, this observation suggests that both classes of fungi release low molecular weight mild oxidants during the first few days of colonization.},
doi = {10.1371/journal.pone.0159715},
journal = {PLoS ONE},
number = 7,
volume = 11,
place = {United States},
year = 2016,
month = 7
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1371/journal.pone.0159715

Citation Metrics:
Cited by: 1work
Citation information provided by
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