A 450 Kb cosmid contig on human chromosome 3p21.3 for cloning tumor suppressor genes associated with lung cancer
- Inc/DynCorp, Frederick, MD (United States); and others
Cytogenetic and molecular analysis of human tumors have strongly suggested that human chromosome 3p21.3 harbors putative tumor suppressor genes which directly contribute to tumorigenesis of small cell lung carcinoma (SCLC). We have constructed a cosmid contig of 450 Kb within 3p21.3. A conventional cosmid walking strategy coupled with Not1 linking and jumping clones and P1 phage clones from 3p21.3 was used to isolate 18 continuous overlapping cosmids. The common overlapping regions of several homozygous deletions in SCLC cell lines are included in this contig. Two approaches are being employed for characterization of this contig: (1) highly conserved expressed sequences are being indentified by utilizing cDNA libraries to identify hybridizing fragments from this contig. Hybridizing genomic fragments are then used to screen cDNA libraries. (2) CpG islands associated with genes are being mapped within each cosmid. Candidate genes obtained in these experiments are being analyzed for mutation in SCLC cell lines by SSCP. This contig also provides a detailed physical map of the region. It will be useful to cloning the putative tumor suppressor gene and systematic characterization of other genes in this region.
- OSTI ID:
- 133598
- Report Number(s):
- CONF-941009--
- Journal Information:
- American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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