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Rapid, portable, multiplexed detection of bacterial pathogens directly from clinical sample matrices

Journal Article · · Biosensors
DOI:https://doi.org/10.3390/bios6040049· OSTI ID:1333619
 [1];  [1];  [2];  [1];  [1]
  1. Sandia National Lab. (SNL-CA), Livermore, CA (United States)
  2. Sandia National Lab. (SNL-CA), Livermore, CA (United States); Cepheid, Sunnyvale, CA (United States)
+ Show Author Affiliations
Enteric and diarrheal diseases are a major cause of childhood illness and death in countries with developing economies. Each year, more than half of a million children under the age of five die from these diseases. We have developed a portable, microfluidic platform capable of simultaneous, multiplexed detection of several of the bacterial pathogens that cause these diseases. Furthermore, this platform can perform fast, sensitive immunoassays directly from relevant, complex clinical matrices such as stool without extensive sample cleanup or preparation. Using only 1 µL of sample per assay, we demonstrate simultaneous multiplexed detection of four bacterial pathogens implicated in diarrheal and enteric diseases in less than 20 min.
Research Organization:
Sandia National Laboratories (SNL-CA), Livermore, CA (United States)
Sponsoring Organization:
NIH; USDOE
Grant/Contract Number:
AC04-94AL85000
OSTI ID:
1333619
Report Number(s):
SAND--2016-8183J; 646818
Journal Information:
Biosensors, Journal Name: Biosensors Journal Issue: 4 Vol. 6; ISSN 2079-6374; ISSN BIOSHU
Publisher:
MDPICopyright Statement
Country of Publication:
United States
Language:
English

References (41)

Molecular Diagnosis of Diarrhea: Current Status and Future Potential journal November 2011
Centrifugal loop-mediated isothermal amplification microdevice for rapid, multiplex and colorimetric foodborne pathogen detection journal January 2016
An integrated direct loop-mediated isothermal amplification microdevice incorporated with an immunochromatographic strip for bacteria detection in human whole blood and milk without a sample preparation step journal May 2016
Real-time loop-mediated isothermal DNA amplification in compact disc micro-reactors journal May 2016
Low-fouling surface plasmon resonance biosensor for multi-step detection of foodborne bacterial pathogens in complex food samples journal June 2016
Effect of Macromolecular Crowding on Protein Binding Stability: Modest Stabilization and Significant Biological Consequences journal August 2009
Centrifugal microfluidic platform for rapid PCR amplification using integrated thermoelectric heating and ice-valving journal January 2012
A microfluidic lab-on-a-disc integrated loop mediated isothermal amplification for foodborne pathogen detection journal May 2016
Global regional and national causes of child mortality journal November 2012
Centrifugal Microfluidic Platform for Ultrasensitive Detection of Botulinum Toxin journal December 2014
Centrifugal microfluidic platforms: advanced unit operations and applications journal January 2015
Rapid and fully automated bacterial pathogen detection on a centrifugal-microfluidic LabDisk using highly sensitive nested PCR with integrated sample preparation journal January 2015
Fully automated and colorimetric foodborne pathogen detection on an integrated centrifugal microfluidic device journal January 2016
Macromolecular crowding effects on protein–protein binding affinity and specificity journal November 2010
Centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water journal January 2016
Efficient and Rapid Detection of Salmonella Using Microfluidic Impedance Based Sensing journal January 2015
Setting Research Priorities to Reduce Mortality and Morbidity of Childhood Diarrhoeal Disease in the Next 15 Years journal May 2013
Global Causes of Diarrheal Disease Mortality in Children <5 Years of Age: A Systematic Review journal September 2013
Whole Blood Immunoassay Based on Centrifugal Bead Sedimentation journal March 2011
A Review of Biomedical Centrifugal Microfluidic Platforms journal February 2016
Challenges in the Use of Compact Disc-Based Centrifugal Microfluidics for Healthcare Diagnostics at the Extreme Point of Care journal March 2016
Combining Electrochemical Sensors with Miniaturized Sample Preparation for Rapid Detection in Clinical Samples text January 2015
Where and why are 10 million children dying every year? journal June 2003
Diarrhoea: why children are still dying and what can be done journal March 2010
Global, regional, and national causes of child mortality: an updated systematic analysis for 2010 with time trends since 2000 journal June 2012
Identifying multiple bacterial pathogens by loop-mediated isothermal amplification on a rotate & react slipchip journal June 2016
Where and why are 10 million children dying every year? journal June 2003
Diarrhoea: why children are still dying and what can be done journal March 2010
Global, regional, and national causes of child mortality: an updated systematic analysis for 2010 with time trends since 2000 journal June 2012
Microfluidic diagnostic technologies for global public health journal July 2006
Centrifugal microfluidic platforms: advanced unit operations and applications journal January 2015
Rapid and fully automated bacterial pathogen detection on a centrifugal-microfluidic LabDisk using highly sensitive nested PCR with integrated sample preparation journal January 2015
Fully automated and colorimetric foodborne pathogen detection on an integrated centrifugal microfluidic device journal January 2016
Centrifugal microfluidics for biomedical applications journal January 2010
A centrifugal fluidic immunoassay for ocular diagnostics with an enzymatically hydrolyzed fluorogenic substrate journal January 2014
Multicenter Evaluation of the BD Max Enteric Bacterial Panel PCR Assay for Rapid Detection of Salmonella spp., Shigella spp., Campylobacter spp. (C. jejuni and C. coli), and Shiga Toxin 1 and 2 Genes journal March 2015
Multicenter Evaluation of the BD Max Enteric Bacterial Panel PCR Assay for Rapid Detection of Salmonella spp., Shigella spp., Campylobacter spp. (C. jejuni and C. coli), and Shiga Toxin 1 and 2 Genes journal March 2015
Advances and challenges in biosensor-based diagnosis of infectious diseases journal February 2014
CD-Based Microfluidics for Primary Care in Extreme Point-of-Care Settings journal January 2016
Combining Electrochemical Sensors with Miniaturized Sample Preparation for Rapid Detection in Clinical Samples journal December 2014
CD-based microfluidics for primary care in extreme point-of-care settings text January 2016

Cited By (2)

Differential DNA accessibility to polymerase enables 30-minute phenotypic β-lactam antibiotic susceptibility testing of carbapenem-resistant Enterobacteriaceae journal March 2020
On-chip stool liquefaction via acoustofluidics journal January 2019

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Related Subjects

47 OTHER INSTRUMENTATION
centrifugal
diagnostics
enteric diseases
immunoassay
microfluidics
pathogen detection
point-of-care