Construction and analysis of a panel of {open_quotes}whole genome{close_quotes} radiation hybrids
- Stanford Univ., CA (United States); and others
In an effort to generate a single panel of radiation hybrid cell lines that can be used to construct a radiation hybrid map of the entire human genome, we have fused diploid male human lymphoblastoid cells exposed to 8000 rad of X-rays with unirradiated Chinese hamster cells deficient for the enzyme thymidine kinase (TK), and selected for radiation hybrids in the presence of HAT selective medium. Genomic DNA isolated from each of 85 independent hybrids was assayed in duplicate for the presence of 100 STSs representing at least two STSs from each of the 23 human chromosomes. The retention frequency of individual markers was found to vary from 5% to 58%, with an average retention of 17%. In addition, each radiation hybrid was scored for the retention of 85 STSs derived from a 25 Mb region of the proximal short arm of chromosome 4. An algorithm developed specifically for use with diploid radiation hybrid data was used to construct a map. Our results indicate that this set of diploid radiation hybrids can be used to construct a radiation hybrid map of the entire human genome at an average resolution of approximately 500kb.
- OSTI ID:
- 133349
- Report Number(s):
- CONF-941009--
- Journal Information:
- American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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