The distribution of 893 STS-markers over the 22 autosomes
- Department of Human Genetics, Salt Lake City, UT (United States); and others
We developed 1009 polymorphic microsatellite markers for the 22 autosomes and built genetic maps from 893 markers. Most of the markers (75%) are tetranucleotides, and 45% have heterozygosities {ge}0.75. The markers were developed from a chromosome non-specific library and were genotyped in four CEPH families (1331, 1332, 1362, and 884). Using the software package LINKAGE, we build the genetic maps in four steps. (I) The localization of each marker to a specific chromosome is determined by 2-point analysis with GENETHON markers. (II) For each chromosome, an anchor map is built from markers with heterozygosities {ge}0.75 and odds {ge}1000:1 for inversion of adjacent loci. (III) More markers are added to the map when odds for inversions are between 100:1 and 1000:1. (IV) Likely locations (1000:1 odds intervals) are determined for markers that cannot be mapped with respect to the above criteria under (II) and (III). 304 markers (34%) were chosen as anchor markers. They cover 3,057 cM of the autosomes (expected length: 4,084 cM). Overall 435 markers (49%) could be linearly ordered and cover 3,206 cM of the chromosomes; the average distance between markers is 7cM, but ranges may vary (e.g. 3-43 cM). Depending on the length of the chromosomes, the number of markers on the (linear) map varies from 5 (chromosome 22) to 42 (chromosome 1). For 458 (51%) markers we determined likely locations on the 22 chromosomes.
- OSTI ID:
- 133345
- Report Number(s):
- CONF-941009--
- Journal Information:
- American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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