Endothelial Antioxidant-1: A key mediator of Copper-dependent wound healing in vivo

Das, Archita; Sudhahar, Varadarajan; Chen, Gin -Fu; Kim, Ha Won; Youn, Seock -Won; Finney, Lydia; Vogt, Stefan; Yang, Jay; Kweon, Junghun; Surenkhuu, Bayasgalan; Ushio-Fukai, Masuko; Fukai, Tohru

doi:10.1038/srep33783

Endothelial Antioxidant-1: A key mediator of Copper-dependent wound healing in vivo

Journal Article · Mon Sep 26 00:00:00 EDT 2016 · Scientific Reports

DOI:https://doi.org/10.1038/srep33783· OSTI ID:1333007

Das, Archita ^[1]; Sudhahar, Varadarajan ^[2]; Chen, Gin -Fu ^[1]; Kim, Ha Won ^[1]; Youn, Seock -Won ^[1]; Finney, Lydia ^[3]; Vogt, Stefan ^[3]; Yang, Jay ^[4]; Kweon, Junghun ^[2]; Surenkhuu, Bayasgalan ^[2]; Ushio-Fukai, Masuko ^[1]; Fukai, Tohru ^[2]

Univ. of Illinois at Chicago, Chicago, IL (United States)
Univ. of Illinois at Chicago, Chicago, IL (United States); Jess Brown Veterans Affairs Medical Center, Chicago, IL (United States)
Argonne National Lab. (ANL), Argonne, IL (United States)
Univ. of Wisconsin, Madison, WI (United States)

Here, Copper (Cu), an essential nutrient, promotes wound healing, however, target of Cu action and underlying mechanisms remains elusive. Cu chaperone Antioxidant-1 (Atox1) in the cytosol supplies Cu to the secretory enzymes such as lysyl oxidase (LOX) while Atox1 in the nucleus functions as a Cu-dependent transcription factor. Using cutaneous wound healing model, here we show that Cu content (by X-ray Fluorescence Microscopy) and nuclear Atox1 are increased after wounding, and that wound healing with and without Cu treatment is impaired in Atox1^-/^- mice. Experiments using endothelial cell (EC)-specific Atox1^-/^- mice and gene transfer of nuclear-target Atox1 in Atox1^-/^- mice reveal that Atox1 in ECs as well as transcription factor function of Atox1 are required for wound healing. Mechanistically, Atox1^-/^- mice show reduced Atox1 target proteins such as p47phox NADPH oxidase and cyclin D1 as well as extracellular matrix Cu enzyme LOX activity in wound tissues. This in turn results in reducing O₂^- production in ECs, NFkB activity, cell proliferation and collagen formation, thereby inhibiting angiogenesis, macrophage recruitment and extracellular matrix maturation. Our findings suggest that Cu-dependent transcription factor/Cu chaperone Atox1 in ECs plays an essential role to sense Cu to accelerate wound angiogenesis and healing.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1333007

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Vol. 6; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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