Taking a look at the calibration of a CCD detector with a fiber-optic taper

Alkire, R. W.; Rotella, F. J.; Duke, Norma E. C.; Otwinowski, Zbyszek; Borek, Dominika M.

doi:10.1107/S1600576716000431

Taking a look at the calibration of a CCD detector with a fiber-optic taper

Journal Article · Mon Feb 15 23:00:00 EST 2016 · Journal of Applied Crystallography (Online)

DOI:https://doi.org/10.1107/S1600576716000431· OSTI ID:1332977

Alkire, R. W. ^[1]; Rotella, F. J. ^[1]; Duke, Norma E. C. ^[1]; Otwinowski, Zbyszek ^[2]; Borek, Dominika M. ^[2]

Argonne National Lab. (ANL), Argonne, IL (United States)
Univ. of Texas Southwestern Medical Center, Dallas, TX (United States)

At the Structural Biology Center beamline 19BM, located at the Advanced Photon Source, the operational characteristics of the equipment are routinely checked to ensure they are in proper working order. After performing a partial flat-field calibration for the ADSC Quantum 210r CCD detector, it was confirmed that the detector operates within specifications. However, as a secondary check it was decided to scan a single reflection across one-half of a detector module to validate the accuracy of the calibration. The intensities from this single reflection varied by more than 30% from the module center to the corner of the module. Redistribution of light within bent fibers of the fiber-optic taper was identified to be a source of this variation. As a result, the degree to which the diffraction intensities are corrected to account for characteristics of the fiber-optic tapers depends primarily upon the experimental strategy of data collection, approximations made by the data processing software during scaling, and crystal symmetry.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1332977

Journal Information:: Journal of Applied Crystallography (Online), Journal Name: Journal of Applied Crystallography (Online) Journal Issue: 2 Vol. 49; ISSN JACGAR; ISSN 1600-5767

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited By (3)

Real-space analysis of radiation-induced specific changes with independent component analysis Borek, Dominika; Bromberg, Raquel; Hattne, Johan Journal of Synchrotron Radiation, Vol. 25, Issue 2 https://doi.org/10.1107/s1600577517018148	journal	February 2018
Real-space analysis of radiation-induced specific changes with independent component analysis Borek, Dominika; Bromberg, Raquel; Hattne, Johan International Union of Crystallography (IUCr) https://doi.org/10.1107/s1600577517018148/gm5051sup4.zip	text	January 2018
Real-space analysis of radiation-induced specific changes with independent component analysis Borek, Dominika; Bromberg, Raquel; Hattne, Johan International Union of Crystallography (IUCr) https://doi.org/10.1107/s1600577517018148/gm5051sup5.zip	text	January 2018

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