Culture-independent diagnostics for health security

Doggett, Norman A.; Mukundan, Harshini; Lefkowitz, Elliot J.; Slezak, Tom R.; Chain, Patrick Sam; Morse, Stephen; Anderson, Kevin; Hodge, David R.; Pillai, Segaran

doi:10.1089/hs.2015.0074

Culture-independent diagnostics for health security

Journal Article · Fri Jun 17 00:00:00 EDT 2016 · Health Security

DOI:https://doi.org/10.1089/hs.2015.0074· OSTI ID:1331269

Doggett, Norman A. ^[1]; Mukundan, Harshini ^[1]; Lefkowitz, Elliot J. ^[2]; Slezak, Tom R. ^[3]; Chain, Patrick Sam ^[1]; Morse, Stephen ^[4]; Anderson, Kevin ^[5]; Hodge, David R. ^[5]; Pillai, Segaran ^[6]

Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Univ. of Alabama at Birmingham, Birmingham, AL (United States)
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Centers for Disease Control and Prevention, Atlanta, GA (United States)
U.S. Department of Homeland Security, Washington, D.C. (United States)
Food and Drug Administration, Silver Spring, MD (United States)

The past decade has seen considerable development in the diagnostic application of nonculture methods, including nucleic acid amplification-based methods and mass spectrometry, for the diagnosis of infectious diseases. The implications of these new culture-independent diagnostic tests (CIDTs) include bypassing the need to culture organisms, thus potentially affecting public health surveillance systems, which continue to use isolates as the basis of their surveillance programs and to assess phenotypic resistance to antimicrobial agents. CIDTs may also affect the way public health practitioners detect and respond to a bioterrorism event. In response to a request from the Department of Homeland Security, Los Alamos National Laboratory and the Centers for Disease Control and Prevention cosponsored a workshop to review the impact of CIDTs on the rapid detection and identification of biothreat agents. Four panel discussions were held that covered nucleic acid amplification–based diagnostics, mass spectrometry, antibody-based diagnostics, and next-generation sequencing. Exploiting the extensive expertise available at this workshop, we identified the key features, benefits, and limitations of the various CIDT methods for providing rapid pathogen identification that are critical to the response and mitigation of a bioterrorism event. After the workshop we conducted a thorough review of the literature, investigating the current state of these 4 culture-independent diagnostic methods. Furthermore, this article combines information from the literature review and the insights obtained at the workshop.

View Accepted Manuscript (DOE)

Research Organization:: Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: U.S. Department of Homeland Security (originally DHS); USDOE

Grant/Contract Number:: AC52-06NA25396

OSTI ID:: 1331269

Report Number(s):: LA-UR--16-20206

Journal Information:: Health Security, Journal Name: Health Security Journal Issue: 3 Vol. 14; ISSN 2326-5094

Publisher:: Mary Ann Liebert, Inc.Copyright Statement

Country of Publication:: United States

Language:: English

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