Uptake and effect of rare earth elements on gene expression in Methylosinus trichosporium OB3b
- Univ. of Michigan, Ann Arbor, MI (United States). Dept. of Civil and Environmental Engineering; The University of Michigan
- Univ. of Michigan, Ann Arbor, MI (United States). Dept. of Civil and Environmental Engineering
- Iowa State Univ., Ames, IA (United States). Dept. of Biochemistry, Biophysics and Molecular Biology
It is well-known that M. trichosporium OB3b has two forms of methane monooxygenase responsible for the initial conversion of methane to methanol, a cytoplasmic (soluble) methane monooxygenase (sMMO) and a membrane-associated (particulate) methane monooxygenase (pMMO) and that copper strongly regulates expression of these alternative forms of MMO. More recently, it has been discovered that M. trichosporium OB3b has multiple types of the methanol dehydrogenase (MeDH), i.e. the Mxa-MeDH and Xox-MeDH, and the expression of these two forms is regulated by the availability of the rare earth element, cerium. Here we extend these studies and show that lanthanum, praseodymium, neodymium and samarium also regulate expression of alternative forms of MeDH. The effect of these rare earth elements on MeDH expression, however, was only observed in the absence of copper. Further, a mutant of M. trichosporium OB3b where the Mxa-MeDH was knocked out was able to grow in the presence of lanthanum, praseodymium and neodymium, but was not able to grow in the presence of samarium. In conclusion, collectively these data suggest that multiple levels of gene regulation by metals exist in M. trichosporium OB3b but that copper overrides the effect of other metals by an as yet unknown mechanism.
- Research Organization:
- Univ. of Michigan, Ann Arbor, MI (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
- Grant/Contract Number:
- SC0006630
- OSTI ID:
- 1329003
- Journal Information:
- FEMS Microbiology Letters (Online), Journal Name: FEMS Microbiology Letters (Online) Journal Issue: 13 Vol. 363; ISSN 1574-6968
- Country of Publication:
- United States
- Language:
- English
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