Non-naturally occurring tRNA.sup.Sec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNA.sup.Sec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNA.sup.Sec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNA.sup.Sec.
Soll, Dieter, et al. "Compositions and methods for making selenocysteine containing polypeptides." US 9,464,288, United States Patent and Trademark Office, Oct. 2016.
Soll, Dieter, Aldag, Caroline, and Hohn, Michael, "Compositions and methods for making selenocysteine containing polypeptides," US 9,464,288, issued October 11, 2016.
@misc{osti_1328702,
author = {Soll, Dieter and Aldag, Caroline and Hohn, Michael},
title = {Compositions and methods for making selenocysteine containing polypeptides},
annote = {Non-naturally occurring tRNA.sup.Sec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNA.sup.Sec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNA.sup.Sec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNA.sup.Sec.},
url = {https://www.osti.gov/biblio/1328702},
place = {United States},
year = {2016},
month = {10},
note = {US Patent