Preclinical Validation of the Heparin-Reactive Peptide p5+14 as a Molecular Imaging Agent for Visceral Amyloidosis

Wall, Jonathan; Martin, Emily B.; Richey, Tina; Stuckey, Alan C.; Macy, Sallie; Wooliver, Craig; Williams, Angela; Foster, James S.; McWilliams-Koeppen, Penney; Uberbacher, Ed; Cheng, Xiaolin; Kennel, Stephen J.

doi:10.3390/molecules20057657

Preclinical Validation of the Heparin-Reactive Peptide p5+14 as a Molecular Imaging Agent for Visceral Amyloidosis

Journal Article · Mon Apr 27 00:00:00 EDT 2015 · Molecules

DOI:https://doi.org/10.3390/molecules20057657· OSTI ID:1327576

Wall, Jonathan ^[1]; Martin, Emily B. ^[2]; Richey, Tina ^[2]; Stuckey, Alan C. ^[3]; Macy, Sallie ^[2]; Wooliver, Craig ^[2]; Williams, Angela ^[2]; Foster, James S. ^[2]; McWilliams-Koeppen, Penney ^[3]; Uberbacher, Ed ^[4]; Cheng, Xiaolin ^[4]; Kennel, Stephen J. ^[1]

Univ. of Tennessee Graduate School of Medicine, Knoxville, TN (United States). Dept. of Medicine; Univ. of Tennessee Graduate School of Medicine, Knoxville, TN (United States). Dept. of and Radiology
Univ. of Tennessee Graduate School of Medicine, Knoxville, TN (United States). Dept. of Medicine
Univ. of Tennessee Graduate School of Medicine, Knoxville, TN (United States). Dept. of and Radiology
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Bioscience Division; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Computer Science and Mathematics Division

Amyloid is a complex pathologic matrix comprised principally of para-crystalline protein fibrils and heparan sulfate proteoglycans. Systemic amyloidoses are rare (~3500 new cases per year in the US); thus, routine diagnosis is often challenging, and effective treatment options are limited, resulting in high morbidity and mortality rates. Glycosaminoglycans contribute inextricably to the formation of amyloid fibrils and foster the deposition of amyloid in tissues. Those present in amyloid deposits are biochemically and electrochemically distinct from glycosaminoglycans found in the plasma membrane and extracellular matrices of healthy tissues due to the presence of a high degree of heparin-like hypersulfation. We have exploited this unique property and evaluated heparin-reactive peptides, such as p5+14. Herein we show efficacious detection of murine systemic amyloid in vivo by using molecular imaging, and the specific targeting of the peptide to major forms of human amyloid in tissue sections. Furthermore, we have demonstrated that the peptide also binds synthetic amyloid fibrils that lack glycosaminoglycans implying that the dense anionic motif present on heparin is mimicked by the amyloid protein fibril itself. These biochemical and functional data support the translation of radiolabeled peptide p5+14 for the clinical imaging of amyloid in patients; thus, providing a novel technique for prognostication, patient stratification, and monitoring response to therapy.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: ORNL LDRD Seed-Money

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1327576

Journal Information:: Molecules, Journal Name: Molecules Journal Issue: 5 Vol. 20; ISSN MOLEFW; ISSN 1420-3049

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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Secondary structure propensity and chirality of the amyloidophilic peptide p5 and its analogues impacts ligand binding - In vitro characterization Wall, Jonathan S.; Williams, Angela; Wooliver, Craig Biochemistry and Biophysics Reports, Vol. 8 https://doi.org/10.1016/j.bbrep.2016.08.007	journal	December 2016
Comparative evaluation of p5+14 with SAP and peptide p5 by dual-energy SPECT imaging of mice with AA amyloidosis Martin, Emily B.; Williams, Angela; Richey, Tina Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep22695	journal	March 2016
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Preclinical Validation of the Heparin-Reactive Peptide p5+14 as a Molecular Imaging Agent for Visceral Amyloidosis

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