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Title: Chemical imaging of molecular changes in a hydrated single cell by dynamic secondary ion mass spectrometry and super-resolution microscopy

Journal Article · · Integrative Biology
DOI:https://doi.org/10.1039/c5ib00308c· OSTI ID:1327108
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Chemical imaging of single cells is important in capturing biological dynamics. Single cell correlative imaging is realized between structured illumination microscopy (SIM) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) using System for Analysis at the Liquid Vacuum Interface (SALVI), a multimodal microreactor. SIM characterized cells and guided subsequent ToF-SIMS analysis. Dynamic ToF-SIMS provided time- and space-resolved cell molecular mapping. Lipid fragments were identified in the hydrated cell membrane. Principal component analysis was used to elucidate chemical component differences among mouse lung cells that uptake zinc oxide nanoparticles. Our results provided submicron chemical spatial mapping for investigations of cell dynamics at the molecular level.

Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC05-76RL01830
OSTI ID:
1327108
Report Number(s):
PNNL-SA-110602; 49143; 48143; 48672; 48671
Journal Information:
Integrative Biology, Vol. 8, Issue 5; ISSN 1757-9694
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English

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correlative chemical imaging • microfluidic • surface analysis • nanoparticle toxicity • principal component analysis
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