Global isotope metabolomics reveals adaptive strategies for nitrogen assimilation

Kurczy, Michael E.; Forsberg, Erica M.; Thorgersen, Michael P.; Poole, Farris L.; Benton, H. Paul; Ivanisevic, Julijana; Tran, Minerva L.; Wall, Judy D.; Elias, Dwayne A.; Adams, Michael W. W.; Siuzdak, Gary

doi:10.1021/acschembio.6b00082

Global isotope metabolomics reveals adaptive strategies for nitrogen assimilation

Journal Article · Tue Apr 05 00:00:00 EDT 2016 · ACS Chemical Biology

DOI:https://doi.org/10.1021/acschembio.6b00082· OSTI ID:1326478

Kurczy, Michael E. ^[1]; Forsberg, Erica M. ^[1]; Thorgersen, Michael P. ^[2]; Poole, Farris L. ^[2]; Benton, H. Paul ^[1]; Ivanisevic, Julijana ^[3]; Tran, Minerva L. ^[1]; Wall, Judy D. ^[4]; Elias, Dwayne A. ^[5]; Adams, Michael W. W. ^[2]; Siuzdak, Gary ^[1]

The Scripps Research Institute, La Jolla, CA (United States)
Univ. of Georgia, Athens, GA (United States)
Univ. of Lausanne, Lausanne (Switzerland)
Univ. of Missouri, Columbia, MO (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Nitrogen cycling is a microbial metabolic process essential for global ecological/agricultural balance. To investigate the link between the well-established ammonium and the alternative nitrate assimilation metabolic pathways, global isotope metabolomics was employed to examine three nitrate reducing bacteria using ¹⁵NO₃ as a nitrogen source. In contrast to a control (Pseudomonas stutzeri RCH2), the results show that two of the isolates from Oak Ridge, Tennessee (Pseudomonas N2A2 and N2E2) utilize nitrate and ammonia for assimilation concurrently with differential labeling observed across multiple classes of metabolites including amino acids and nucleotides. The data reveal that the N2A2 and N2E2 strains conserve nitrogen-containing metabolites, indicating that the nitrate assimilation pathway is a conservation mechanism for the assimilation of nitrogen. Co-utilization of nitrate and ammonia is likely an adaption to manage higher levels of nitrite since the denitrification pathways utilized by the N2A2 and N2E2 strains from the Oak Ridge site are predisposed to the accumulation of the toxic nitrite. In conclusion, the use of global isotope metabolomics allowed for this adaptive strategy to be investigated, which would otherwise not have been possible to decipher.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1326478

Journal Information:: ACS Chemical Biology, Journal Name: ACS Chemical Biology Journal Issue: 6 Vol. 11; ISSN 1554-8929

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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