Two-stage dilute-acid and organic-solvent lignocellulosic pretreatment for enhanced bioprocessing

Brodeur, G.; Telotte, J.; Stickel, J. J.; Ramakrishnan, S.

doi:10.1016/j.biortech.2016.08.089

Two-stage dilute-acid and organic-solvent lignocellulosic pretreatment for enhanced bioprocessing

Journal Article · Fri Aug 26 00:00:00 EDT 2016 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2016.08.089· OSTI ID:1326330

Brodeur, G. ^[1]; Telotte, J. ^[1]; Stickel, J. J. ^[2]; Ramakrishnan, S. ^[1]

Florida A&M Univ. - Florida State Univ., Tallahassee, FL (United States). College of Engineering
National Renewable Energy Lab. (NREL), Golden, CO (United States)

A two stage pretreatment approach for biomass is developed in the current work in which dilute acid (DA) pretreatment is followed by a solvent based pretreatment (N-methyl morpholine N oxide -- NMMO). When the combined pretreatment (DAWNT) is applied to sugarcane bagasse and corn stover, the rates of hydrolysis and overall yields (>90%) are seen to dramatically improve and under certain conditions 48 h can be taken off the time of hydrolysis with the additional NMMO step to reach similar conversions. DAWNT shows a 2-fold increase in characteristic rates and also fractionates different components of biomass -- DA treatment removes the hemicellulose while the remaining cellulose is broken down by enzymatic hydrolysis after NMMO treatment to simple sugars. The remaining residual solid is high purity lignin. Lastly, future work will focus on developing a full scale economic analysis of DAWNT for use in biomass fractionation.

View Accepted Manuscript (DOE)

Research Organization:: National Renewable Energy Lab. (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE; USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1326330

Alternate ID(s):: OSTI ID: 1396350

Report Number(s):: NREL/JA--5100-66759

Journal Information:: Bioresource Technology, Journal Name: Bioresource Technology Journal Issue: C Vol. 220; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

References (31)

Rheology of cellulosicN-methylmorpholine oxide monohydrate solutions of different degrees of polymerization Petrovan, S.; Collier, J. R.; Negulescu, I. I. Journal of Applied Polymer Science, Vol. 79, Issue 3 https://doi.org/10.1002/1097-4628(20010118)79:3<396::AID-APP20>3.0.CO;2-E	journal	January 2000
Enhancement of cellulose saccharification kinetics using an ionic liquid pretreatment step Dadi, Anantharam P.; Varanasi, Sasidhar; Schall, Constance A. Biotechnology and Bioengineering, Vol. 95, Issue 5, p. 904-910 https://doi.org/10.1002/bit.21047	journal	January 2006
Enhancement of ethanol and biogas production from high-crystalline cellulose by different modes of NMO pretreatment Jeihanipour, Azam; Karimi, Keikhosro; Taherzadeh, Mohammad J. Biotechnology and Bioengineering, Vol. 105, Issue 3 https://doi.org/10.1002/bit.22558	journal	February 2010
Kinetic study on enzymatic hydrolysis of cellulose by cellulose fromTrichoderma viride Ohmine, Kazuo; Ooshima, Hiroshi; Harano, Yoshio Biotechnology and Bioengineering, Vol. 25, Issue 8 https://doi.org/10.1002/bit.260250813	journal	August 1983
Correlating the effect of pretreatment on the enzymatic hydrolysis of straw Koullas, D. P.; Christakopoulos, P.; Kekos, D. Biotechnology and Bioengineering, Vol. 39, Issue 1 https://doi.org/10.1002/bit.260390116	journal	January 1992
Comparisons of SPORL and dilute acid pretreatments for sugar and ethanol productions from aspen Tian, S.; Zhu, W.; Gleisner, R. Biotechnology Progress, Vol. 27, Issue 2 https://doi.org/10.1002/btpr.545	journal	January 2011
Comparison of ionic liquid, acid and alkali pretreatments for sugarcane bagasse enzymatic saccharification Yoon, Li Wan; Ngoh, Gek Cheng; May Chua, Adeline Seak Journal of Chemical Technology & Biotechnology, Vol. 86, Issue 10 https://doi.org/10.1002/jctb.2651	journal	June 2011
Effect of solids loading and solvent type on the enzymatic hydrolysis of cellulose: Effect of solids loading and solvent on enzymatic hydrolysis of cellulose Brodeur, Gary; Yau, Elizabeth; Collier, John Journal of Chemical Technology & Biotechnology, Vol. 92, Issue 1 https://doi.org/10.1002/jctb.4972	journal	April 2016
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, Marion M. Analytical Biochemistry, Vol. 72, Issue 1-2 https://doi.org/10.1016/0003-2697(76)90527-3	journal	May 1976
Dilute mixed-acid pretreatment of sugarcane bagasse for ethanol production Jackson de Moraes Rocha, George; Martin, Carlos; Soares, Isaias Barbosa Biomass and Bioenergy, Vol. 35, Issue 1 https://doi.org/10.1016/j.biombioe.2010.10.018	journal	January 2011
Enzymatic hydrolysis of lime-pretreated corn stover and investigation of the HCH-1 Model: Inhibition pattern, degree of inhibition, validity of simplified HCH-1 Model O’Dwyer, Jonathan P.; Zhu, Li; Granda, Cesar B. Bioresource Technology, Vol. 98, Issue 16 https://doi.org/10.1016/j.biortech.2006.10.014	journal	November 2007
A comparison of chemical pretreatment methods for improving saccharification of cotton stalks Silverstein, Rebecca A.; Chen, Ye; Sharma-Shivappa, Ratna R. Bioresource Technology, Vol. 98, Issue 16 https://doi.org/10.1016/j.biortech.2006.10.022	journal	November 2007
Pretreatment of spruce and oak by N-methylmorpholine-N-oxide (NMMO) for efficient conversion of their cellulose to ethanol Shafiei, Marzieh; Karimi, Keikhosro; Taherzadeh, Mohammad J. Bioresource Technology, Vol. 101, Issue 13 https://doi.org/10.1016/j.biortech.2009.08.100	journal	July 2010
Enzymatic hydrolysis of cellulose dissolved in N-methyl morpholine oxide/water solutions Ramakrishnan, S.; Collier, J.; Oyetunji, R. Bioresource Technology, Vol. 101, Issue 13 https://doi.org/10.1016/j.biortech.2009.09.002	journal	July 2010
Optimizing the saccharification of sugar cane bagasse using dilute phosphoric acid followed by fungal cellulases Geddes, C. C.; Peterson, J. J.; Roslander, C. Bioresource Technology, Vol. 101, Issue 6, p. 1851-1857 https://doi.org/10.1016/j.biortech.2009.09.070	journal	March 2010
Comparison of dilute acid and ionic liquid pretreatment of switchgrass: Biomass recalcitrance, delignification and enzymatic saccharification Li, Chenlin; Knierim, Bernhard; Manisseri, Chithra Bioresource Technology, Vol. 101, Issue 13 https://doi.org/10.1016/j.biortech.2009.10.066	journal	July 2010
Effects of dilute acid pretreatment conditions on enzymatic hydrolysis monomer and oligomer sugar yields for aspen, balsam, and switchgrass Jensen, Jill R.; Morinelly, Juan E.; Gossen, Kelsey R. Bioresource Technology, Vol. 101, Issue 7 https://doi.org/10.1016/j.biortech.2009.11.038	journal	April 2010
Structural comparison and enhanced enzymatic hydrolysis of the cellulosic preparation from Populus tomentosa Carr., by different cellulose-soluble solvent systems Wang, Kun; Yang, Hai Yan; Xu, Feng Bioresource Technology, Vol. 102, Issue 6 https://doi.org/10.1016/j.biortech.2010.12.088	journal	March 2011
A pilot study on lignocelluloses to ethanol and fish feed using NMMO pretreatment and cultivation with zygomycetes in an air-lift reactor Lennartsson, Patrik R.; Niklasson, Claes; Taherzadeh, Mohammad J. Bioresource Technology, Vol. 102, Issue 6 https://doi.org/10.1016/j.biortech.2010.12.089	journal	March 2011
Comparative data on effects of leading pretreatments and enzyme loadings and formulations on sugar yields from different switchgrass sources Wyman, Charles E.; Balan, Venkatesh; Dale, Bruce E. Bioresource Technology, Vol. 102, Issue 24 https://doi.org/10.1016/j.biortech.2011.06.069	journal	December 2011
Elucidation of cellulose accessibility, hydrolysability and reactivity as the major limitations in the enzymatic hydrolysis of cellulose Bansal, Prabuddha; Vowell, Bryan J.; Hall, Mélanie Bioresource Technology, Vol. 107 https://doi.org/10.1016/j.biortech.2011.12.063	journal	March 2012
Combining treatments to improve the fermentation of sugarcane bagasse hydrolysates by ethanologenic Escherichia coli LY180 Geddes, Ryan; Shanmugam, Keelnatham T.; Ingram, Lonnie O. Bioresource Technology, Vol. 189 https://doi.org/10.1016/j.biortech.2015.03.141	journal	August 2015
Mutation in galP improved fermentation of mixed sugars to succinate using engineered Escherichia coli AS1600a and AM1 mineral salts medium Sawisit, Apichai; Jantama, Kaemwich; Zheng, Huabao Bioresource Technology, Vol. 193 https://doi.org/10.1016/j.biortech.2015.06.108	journal	October 2015
Enhancement of enzymatic saccharification of cellulose by cellulose dissolution pretreatments Kuo, Chia-Hung; Lee, Cheng-Kang Carbohydrate Polymers, Vol. 77, Issue 1 https://doi.org/10.1016/j.carbpol.2008.12.003	journal	May 2009
Improvement of saccharification and ethanol production from rice straw by NMMO and [BMIM][OAc] pretreatments Poornejad, Nafiseh; Karimi, Keikhosro; Behzad, Tayebeh Industrial Crops and Products, Vol. 41 https://doi.org/10.1016/j.indcrop.2012.04.059	journal	January 2013
Construction and demolition lignocellulosic wastes to bioethanol Jafari, Vahid; Labafzadeh, Sara Rahim; Jeihanipour, Azam Renewable Energy, Vol. 36, Issue 11 https://doi.org/10.1016/j.renene.2011.04.028	journal	November 2011
Methods for Pretreatment of Lignocellulosic Biomass for Efficient Hydrolysis and Biofuel Production Kumar, Parveen; Barrett, Diane M.; Delwiche, Michael J. Industrial & Engineering Chemistry Research, Vol. 48, Issue 8, p. 3713-3729 https://doi.org/10.1021/ie801542g	journal	April 2009
Engineering furfural tolerance in Escherichia coli improves the fermentation of lignocellulosic sugars into renewable chemicals Wang, X.; Yomano, L. P.; Lee, J. Y. Proceedings of the National Academy of Sciences, Vol. 110, Issue 10, p. 4021-4026 https://doi.org/10.1073/pnas.1217958110	journal	February 2013
Measurement of cellulase activities Ghose, T. K. Pure and Applied Chemistry, Vol. 59, Issue 2 https://doi.org/10.1351/pac198759020257	journal	January 1987
Effect of Sulfuric and Phosphoric Acid Pretreatments on Enzymatic Hydrolysis of Corn Stover Um, Byung-Hwan; Karim, M. Nazmul; Henk, Linda L. Applied Biochemistry and Biotechnology, Vol. 105, Issue 1-3 https://doi.org/10.1385/ABAB:105:1-3:115	journal	January 2003
Chemical and Physicochemical Pretreatment of Lignocellulosic Biomass: A Review Brodeur, Gary; Yau, Elizabeth; Badal, Kimberly Enzyme Research, Vol. 2011 https://doi.org/10.4061/2011/787532	journal	January 2011

Cited By (1)

A two-stage pretreatment using acidic dioxane followed by dilute hydrochloric acid on sugar production from corn stover An, Shengxin; Li, Wenzhi; Liu, Qiyu RSC Advances, Vol. 7, Issue 52 https://doi.org/10.1039/c7ra05280d	journal	January 2017

Similar Records

Combined Dilute Acid and Solvent Based Pretreatment of Agricultural Wastes for Efficient Lignocellulosic Fractionation and Biofuels Production

Conference · Mon Dec 31 23:00:00 EST 2012 · OSTI ID:1087249

Comparative Study of Corn Stover Pretreated by Dilute Acid and Cellulose Solvent-Based Lignocellulose Fractionation: Enzymatic Hydrolysis, Supramolecular Structure, and Substrate Accessibility

Journal Article · Wed Jul 01 00:00:00 EDT 2009 · Biotechnology and Bioengineering · OSTI ID:968463

Ammonia Fiber Expansion (AFEX) Pretreatment of Lignocellulosic Biomass

Journal Article · Fri Apr 17 20:00:00 EDT 2020 · Journal of Visualized Experiments · OSTI ID:1637459

Related Subjects

09 BIOMASS FUELS
biomass to biofuels
cellulose
enzymatic hydrolysis
multi stage treatment

Two-stage dilute-acid and organic-solvent lignocellulosic pretreatment for enhanced bioprocessing

Citation Formats

References (31)

Cited By (1)

Similar Records

Related Subjects