Comparative Study of Effects of CO2 Concentration and pH on Microbial Communities from a Saline Aquifer, a Depleted Oil Reservoir, and a Freshwater Aquifer

Gulliver, Djuna M.; Lowry, Gregory V.; Gregory, Kelvin B.

doi:10.1089/ees.2015.0368

Comparative Study of Effects of CO₂ Concentration and pH on Microbial Communities from a Saline Aquifer, a Depleted Oil Reservoir, and a Freshwater Aquifer

Journal Article · Tue Aug 09 00:00:00 EDT 2016 · Environmental Engineering Science

DOI:https://doi.org/10.1089/ees.2015.0368· OSTI ID:1307331

Gulliver, Djuna M. ^[1]; Lowry, Gregory V. ^[2]; Gregory, Kelvin B. ^[2]

National Energy Technology Lab. (NETL), Pittsburgh, PA, (United States). Office of Research and Development
Carnegie Mellon Univ., Pittsburgh, PA (United States). Dept. of Civil and Environmental Engineering

Injected CO₂ from geologic carbon storage is expected to impact the microbial communities of proposed storage sites, such as depleted oil reservoirs and deep saline aquifers, as well as overlying freshwater aquifers at risk of receiving leaking CO₂. Microbial community change in these subsurface sites may affect injectivity of CO₂, permanence of stored CO₂, and shallow subsurface water quality. The effect of CO₂ concentration on the microbial communities in fluid collected from a depleted oil reservoir and a freshwater aquifer was examined at subsurface pressures and temperatures. The community was exposed to 0%, 1%, 10%, and 100% pCO₂ for 56 days. Bacterial community structure was analyzed through 16S rRNA gene clone libraries, and total bacterial abundance was estimated through quantitative polymerase chain reaction. Changes in the microbial community observed in the depleted oil reservoir samples and freshwater samples were compared to previous results from CO₂-exposed deep saline aquifer fluids. Overall, results suggest that CO₂ exposure to microbial communities will result in pH-dependent population change, and the CO₂-selected microbial communities will vary among sites. In conclusion, this is the first study to compare the response of multiple subsurface microbial communities at conditions expected during geologic carbon storage, increasing the understanding of environmental drivers for microbial community changes in CO₂-exposed environments.

View Accepted Manuscript (DOE)

Research Organization:: National Energy Technology Lab. (NETL), Albany, OR (United States)

Sponsoring Organization:: USDOE

OSTI ID:: 1307331

Report Number(s):: NETL-PUB--20090

Journal Information:: Environmental Engineering Science, Journal Name: Environmental Engineering Science Journal Issue: 10 Vol. 33; ISSN 1092-8758

Publisher:: Mary Ann Liebert, Inc.Copyright Statement

Country of Publication:: United States

Language:: English

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54 ENVIRONMENTAL SCIENCES
Subsurface microbial communities
carbon sequestration
geologic carbon storage