Highly specific gene silencing in a monocot species by artificial microRNAs derived from chimeric miRNA precursors

Carbonell, Alberto; Fahlgren, Noah; Mitchell, Skyler; Cox, Jr, Kevin L.; Reilly, Kevin C.; Mockler, Todd C.; Carrington, James C.

doi:10.1111/tpj.12835

Title: Highly specific gene silencing in a monocot species by artificial microRNAs derived from chimeric miRNA precursors

Journal Article · Wed May 20 00:00:00 EDT 2015 · The Plant Journal

DOI:https://doi.org/10.1111/tpj.12835· OSTI ID:1295954

Carbonell, Alberto ^[1]; Fahlgren, Noah ^[1]; Mitchell, Skyler ^[1]; Cox, Jr, Kevin L. ^[1]; Reilly, Kevin C. ^[1]; Mockler, Todd C. ^[1]; Carrington, James C. ^[1]

Donald Danforth Plant Science Center, St. Louis MO 63132 USA

Artificial microRNAs (amiRNAs) are used for selective gene silencing in plants. However, current methods to produce amiRNA constructs for silencing transcripts in monocot species are not suitable for simple, cost-effective and large-scale synthesis. Here, a series of expression vectors based on Oryza sativa MIR390 (OsMIR390) precursor was developed for high-throughput cloning and high expression of amiRNAs in monocots. Four different amiRNA sequences designed to target specifically endogenous genes and expressed from OsMIR390-based vectors were validated in transgenic Brachypodium distachyon plants. Surprisingly, amiRNAs accumulated to higher levels and were processed more accurately when expressed from chimeric OsMIR390-based precursors that include distal stem-loop sequences from Arabidopsis thaliana MIR390a (AtMIR390a). In all cases, transgenic plants displayed the predicted phenotypes induced by target gene repression, and accumulated high levels of amiRNAs and low levels of the corresponding target transcripts. Genome-wide transcriptome profiling combined with 5-RLM-RACE analysis in transgenic plants confirmed that amiRNAs were highly specific. Finally, significance Statement A series of amiRNA vectors based on Oryza sativa MIR390 (OsMIR390) precursor were developed for simple, cost-effective and large-scale synthesis of amiRNA constructs to silence genes in monocots. Unexpectedly, amiRNAs produced from chimeric OsMIR390-based precursors including Arabidopsis thaliana MIR390a distal stem-loop sequences accumulated elevated levels of highly effective and specific amiRNAs in transgenic Brachypodium distachyon plants.

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Research Organization:: Donald Danforth Plant Science Center, St. Louis, MO (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Institutes of Health (NIH); National Science Foundation (NSF)

Grant/Contract Number:: SC0006627; MCB-1231726; MCB-1330562; AI043288

OSTI ID:: 1295954

Alternate ID(s):: OSTI ID: 1295955; OSTI ID: 1457329

Journal Information:: The Plant Journal, Journal Name: The Plant Journal Vol. 82 Journal Issue: 6; ISSN 0960-7412

Publisher:: Society for Experimental BiologyCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 32 works

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59 BASIC BIOLOGICAL SCIENCES
RNA silencing
artificial microRNA
MIRNA precursor
Brachypodium distachyon
monocot
Arabidopsis thaliana
technical advance

Title: Highly specific gene silencing in a monocot species by artificial microRNAs derived from chimeric miRNA precursors

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