Steering microtubule shuttle transport with dynamically controlled magnetic fields

Mahajan, K. D.; Ruan, G.; Dorcéna, C. J.; Vieira, G.; Nabar, G.; Bouxsein, N. F.; Chalmers, J. J.; Bachand, G. D.; Sooryakumar, R.; Winter, J. O.

doi:10.1039/c5nr08529b

Steering microtubule shuttle transport with dynamically controlled magnetic fields

Journal Article · Wed Mar 23 00:00:00 EDT 2016 · Nanoscale

DOI:https://doi.org/10.1039/c5nr08529b· OSTI ID:1285955

Mahajan, K. D. ^[1]; Ruan, G. ^[2]; Dorcéna, C. J. ^[1]; Vieira, G. ^[3]; Nabar, G. ^[1]; Bouxsein, N. F. ^[4]; Chalmers, J. J. ^[1]; Bachand, G. D. ^[4]; Sooryakumar, R. ^[3]; Winter, J. O. ^[5]

The Ohio State Univ., Columbus, OH (United States). William G. Lowrie Dept. of Chemical and Biomolecular Engineering
The Ohio State Univ., Columbus, OH (United States). William G. Lowrie Dept. of Chemical and Biomolecular Engineering; Nanjing Univ. (China). College of Engineering and Applied Sciences, Dept. of Biomedical Engineering
The Ohio State Univ., Columbus, OH (United States). Dept of Physics
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Center for Integrated Nanotechnologies
The Ohio State Univ., Columbus, OH (United States). William G. Lowrie Dept. of Chemical and Biomolecular Engineering; The Ohio State Univ., Columbus, OH (United States). Dept of Biomedical Engineering

Nanoscale control of matter is critical to the design of integrated nanosystems. Here, we describe a method to dynamically control directionality of microtubule (MT) motion using programmable magnetic fields. MTs are combined with magnetic quantum dots (i.e., MagDots) that are manipulated by external magnetic fields provided by magnetic nanowires. MT shuttles thus undergo both ATP-driven and externally-directed motion with a fluorescence component that permits simultaneous visualization of shuttle motion. This technology is used to alter the trajectory of MTs in motion and to pin MT motion. Ultimately, such an approach could be used to evaluate the MT-kinesin transport system and could serve as the basis for improved lab-on-a-chip technologies based on MT transport.

View Accepted Manuscript (DOE)

Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC04-94AL85000

OSTI ID:: 1285955

Report Number(s):: SAND2016--7202J; 646152

Journal Information:: Nanoscale, Journal Name: Nanoscale Journal Issue: 16 Vol. 8; ISSN NANOHL; ISSN 2040-3364

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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