A directed-overflow and damage-control N -glycosidase in riboflavin biosynthesis

Frelin, Océane; Huang, Lili; Hasnain, Ghulam; Jeffryes, James G.; Ziemak, Michael J.; Rocca, James R.; Wang, Bing; Rice, Jennifer; Roje, Sanja; Yurgel, Svetlana N.; Gregory, Jesse F.; Edison, Arthur S.; Henry, Christopher  S.; de Crécy-Lagard, Valérie; Hanson, Andrew D.

doi:10.1042/bj20141237

Title: A directed-overflow and damage-control N -glycosidase in riboflavin biosynthesis

Journal Article · Sun Feb 15 00:00:00 EST 2015 · Biochemical Journal

DOI:https://doi.org/10.1042/bj20141237· OSTI ID:1280875

Frelin, Océane ^[1]; Huang, Lili ^[2]; Hasnain, Ghulam ^[1]; Jeffryes, James G. ^[3]; Ziemak, Michael J. ^[1]; Rocca, James R. ^[4]; Wang, Bing ^[5]; Rice, Jennifer ^[6]; Roje, Sanja ^[6]; Yurgel, Svetlana N. ^[6]; Gregory, Jesse F. ^[2]; Edison, Arthur S. ^[5]; Henry, Christopher S. ^[7]; de Crécy-Lagard, Valérie ^[8]; Hanson, Andrew D. ^[1]

Univ. of Florida, Gainesville, FL (United States). Horticultural Sciences Dept.
Univ. of Florida, Gainesville, FL (United States). Food Science and Human Nutrition Dept.
Northwestern Univ., Evanston, IL (United States). Dept. of Chemical and Biological Engineering; Argonne National Lab. (ANL), Argonne, IL (United States). Mathematics and Computer Science Division
Univ. of Florida, Gainesville, FL (United States). McKnight Brain Inst.
Univ. of Florida, Gainesville, FL (United States). Dept. of Biochemistry and Molecular Biology
Washington State Univ., Pullman, WA (United States). Inst. of Biological Chemistry
Argonne National Lab. (ANL), Argonne, IL (United States). Mathematics and Computer Science Division; Univ. of Chicago, IL (United States). Computation Inst.
Univ. of Florida, Gainesville, FL (United States). Dept. of Microbiology and Cell Science

Plants and bacteria synthesize the essential human micronutrient riboflavin (vitamin B2) via the same multistep pathway. The early intermediates of this pathway are notoriously reactive, and may be overproduced in vivo because riboflavin biosynthesis enzymes lack feedback controls. Here we demonstrate disposal of riboflavin intermediates by COG3236 (DUF1768), a protein of previously unknown function that is fused to two different riboflavin pathway enzymes in plants and bacteria (RIBR and RibA, respectively). We present cheminformatic, biochemical, genetic, and genomic evidence to show that: (i) plant and bacterial COG3236 proteins cleave the N-glycosidic bond of the first two intermediates of riboflavin biosynthesis, yielding relatively innocuous products; (ii) certain COG3236 proteins are in a multienzyme riboflavin biosynthesis complex that gives them privileged access to riboflavin intermediates; and (iii) COG3236 action in Arabidopsis thaliana and Escherichia coli helps maintain flavin levels. We find COG3236 proteins thus illustrate two emerging principles in chemical biology: directed overflow metabolism, in which excess flux is diverted out of a pathway, and the pre-emption of damage from reactive metabolites.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE; National Science Foundation (NSF)

Grant/Contract Number:: IOS-1025398; 1U24DK097209-01A1; MCB-1153413; MCB-1153357

OSTI ID:: 1280875

Journal Information:: Biochemical Journal, Vol. 466, Issue 1; ISSN 0264-6021

Publisher:: Biochemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 34 works

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