skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: 15 N- and 2 H proteomic stable isotope probing links nitrogen flow to archaeal heterotrophic activity

Abstract

Understanding how individual species contribute to nutrient transformations in a microbial community is critical to prediction of overall ecosystem function. We conducted microcosm experiments in which floating acid mine drainage (AMD) microbial biofilms were submerged recapitulating the final stage in a natural biofilm life cycle. Biofilms were amended with either 15NH4 + or deuterium oxide (2H2O) and proteomic stable isotope probing (SIP) was used to track the extent to which different members of the community used these molecules in protein synthesis across anaerobic iron-reducing, aerobic iron-reducing and aerobic iron-oxidizing environments. Sulfobacillus spp. synthesized 15N-enriched protein almost exclusively under iron-reducing conditions whereas the Leptospirillum spp. synthesized 15N-enriched protein in all conditions. There were relatively few 15Nenriched archaeal proteins, and all showed low atom% enrichment, consistent with Archaea synthesizing protein using the predominantly 14N biomass derived from recycled biomolecules. In parallel experiments using 2H2O, extensive archaeal protein synthesis was detected in all conditions. In contrast, the bacterial species showed little protein synthesis using 2H2O. The nearly exclusive ability of Archaea to synthesize proteins using 2H2O may be due to archaeal heterotrophy, whereby Archaea off set deleterious effects of 2H by accessing 1H generated byrespiration of organic compounds.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1]
  1. Univ. of California, Berkeley, CA (United States)
Publication Date:
Research Org.:
Oak Ridge National Laboratory, Oak Ridge Leadership Computing Facility (OLCF); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1265333
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article
Journal Name:
Environmental Microbiology
Additional Journal Information:
Journal Volume: 16; Journal Issue: 10; Journal ID: ISSN 1462-2912
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Justice, Nicholas B., Li, Zhou, Wang, Yingfeng, Spaulding, Susan E., Mosier, Annika C., Robert L. Hettich, Pan, Chongle, and Banfield, Jillian F. 15 N- and 2 H proteomic stable isotope probing links nitrogen flow to archaeal heterotrophic activity. United States: N. p., 2014. Web. doi:10.1111/1462-2920.12488.
Justice, Nicholas B., Li, Zhou, Wang, Yingfeng, Spaulding, Susan E., Mosier, Annika C., Robert L. Hettich, Pan, Chongle, & Banfield, Jillian F. 15 N- and 2 H proteomic stable isotope probing links nitrogen flow to archaeal heterotrophic activity. United States. doi:10.1111/1462-2920.12488.
Justice, Nicholas B., Li, Zhou, Wang, Yingfeng, Spaulding, Susan E., Mosier, Annika C., Robert L. Hettich, Pan, Chongle, and Banfield, Jillian F. Tue . "15 N- and 2 H proteomic stable isotope probing links nitrogen flow to archaeal heterotrophic activity". United States. doi:10.1111/1462-2920.12488.
@article{osti_1265333,
title = {15 N- and 2 H proteomic stable isotope probing links nitrogen flow to archaeal heterotrophic activity},
author = {Justice, Nicholas B. and Li, Zhou and Wang, Yingfeng and Spaulding, Susan E. and Mosier, Annika C. and Robert L. Hettich and Pan, Chongle and Banfield, Jillian F.},
abstractNote = {Understanding how individual species contribute to nutrient transformations in a microbial community is critical to prediction of overall ecosystem function. We conducted microcosm experiments in which floating acid mine drainage (AMD) microbial biofilms were submerged recapitulating the final stage in a natural biofilm life cycle. Biofilms were amended with either 15NH4 + or deuterium oxide (2H2O) and proteomic stable isotope probing (SIP) was used to track the extent to which different members of the community used these molecules in protein synthesis across anaerobic iron-reducing, aerobic iron-reducing and aerobic iron-oxidizing environments. Sulfobacillus spp. synthesized 15N-enriched protein almost exclusively under iron-reducing conditions whereas the Leptospirillum spp. synthesized 15N-enriched protein in all conditions. There were relatively few 15Nenriched archaeal proteins, and all showed low atom% enrichment, consistent with Archaea synthesizing protein using the predominantly 14N biomass derived from recycled biomolecules. In parallel experiments using 2H2O, extensive archaeal protein synthesis was detected in all conditions. In contrast, the bacterial species showed little protein synthesis using 2H2O. The nearly exclusive ability of Archaea to synthesize proteins using 2H2O may be due to archaeal heterotrophy, whereby Archaea off set deleterious effects of 2H by accessing 1H generated byrespiration of organic compounds.},
doi = {10.1111/1462-2920.12488},
journal = {Environmental Microbiology},
issn = {1462-2912},
number = 10,
volume = 16,
place = {United States},
year = {2014},
month = {5}
}

Works referenced in this record:

Ferrozine---a new spectrophotometric reagent for iron
journal, June 1970

  • Stookey, Lawrence L.
  • Analytical Chemistry, Vol. 42, Issue 7, p. 779-781
  • DOI: 10.1021/ac60289a016

Stable-isotope probing as a tool in microbial ecology
journal, February 2000

  • Radajewski, Stefan; Ineson, Philip; Parekh, Nisha R.
  • Nature, Vol. 403, Issue 6770, p. 646-649
  • DOI: 10.1038/35001054

DNA-based stable isotope probing: a link between community structure and function
journal, June 2009

  • Uhlík, Ondrej; Jecná, Katerina; Leigh, Mary Beth
  • Science of The Total Environment, Vol. 407, Issue 12, p. 3611-3619
  • DOI: 10.1016/j.scitotenv.2008.05.012