Selection of short peptides with high affinity for various metal ions: Strategies and applications
- Univ. of Pittsburg, PA (United States)
The authors have used a random combinatorial library to generate an immensely diverse population of candidate peptides and have screened the library, using novel strategies, for selection of peptides of displaying high affinity towards a target metal. As the pool of available binding candidates, we have employed a hexapeptide library, containing essentially all the possible combination of sequences for a hexapeptide, expressed on surface of phage. Our experiments with copper demonstrated that our amplification and selection procedure is very effective. Out of 20 clones sequenced, after four rounds of amplification and selection 18 shared the same sequence. The corresponding peptide which contain two histidines was then synthesized and its binding affinity for copper was assessed. The results revealed that the selected peptide has a very high affinity for copper; its affinity is significantly higher than any hexapeptide which we are aware of including six adjacent histidine, (his)6, frequently used as an affinity tag in bioseparation. Selection of short peptides with high affinity and selectivity for arsenic, mercury, and lead is also underway. We envision important application of this work including discovery of highly effective drugs for treating metal toxicity replacing the chelating agents currently in use, and development of inexpensive whole cell adsorbents for toxicity replacing the chelating agents currently in use, and development of inexpensive whole cell adsorbents for the removal of toxic metals from dilute aqueous streams because dead cells or isolated cell wall, covered by the desired metal-binding domain may serve as high affinity and high capacity metal adsorbents. Other potential application will also be discussed in this presentation.
- OSTI ID:
- 126139
- Report Number(s):
- CONF-950402-; TRN: 95:006086-0112
- Resource Relation:
- Conference: 209. American Chemical Society (ACS) national meeting, Anaheim, CA (United States), 2-6 Apr 1995; Other Information: PBD: 1995; Related Information: Is Part Of 209th ACS national meeting; PB: 2088 p.
- Country of Publication:
- United States
- Language:
- English
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