Autophagic Degradation of the 26S Proteasome Is Mediated by the Dual ATG8/Ubiquitin Receptor RPN10 in Arabidopsis

Marshall, Richard S.; Li, Faqiang; Gemperline, David C.; Book, Adam J.; Vierstra, Richard D.

doi:10.1016/j.molcel.2015.04.023

Title: Autophagic Degradation of the 26S Proteasome Is Mediated by the Dual ATG8/Ubiquitin Receptor RPN10 in Arabidopsis

Journal Article · Mon Jun 01 00:00:00 EDT 2015 · Molecular Cell

DOI:https://doi.org/10.1016/j.molcel.2015.04.023· OSTI ID:1259320

Marshall, Richard S.; Li, Faqiang; Gemperline, David C.; Book, Adam J.; Vierstra, Richard D.

Autophagic turnover of intracellular constituents is critical for cellular housekeeping, nutrient recycling, and various aspects of growth and development in eukaryotes. In this paper, we show that autophagy impacts the other major degradative route involving the ubiquitin-proteasome system by eliminating 26S proteasomes, a process we termed proteaphagy. Using Arabidopsis proteasomes tagged with GFP, we observed their deposition into vacuoles via a route requiring components of the autophagy machinery. This transport can be initiated separately by nitrogen starvation and chemical or genetic inhibition of the proteasome, implying distinct induction mechanisms. Proteasome inhibition stimulates comprehensive ubiquitylation of the complex, with the ensuing proteaphagy requiring the proteasome subunit RPN10, which can simultaneously bind both ATG8 and ubiquitin. Finally and collectively, we propose that Arabidopsis RPN10 acts as a selective autophagy receptor that targets inactive 26S proteasomes by concurrent interactions with ubiquitylated proteasome subunits/targets and lipidated ATG8 lining the enveloping autophagic membranes.

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Research Organization:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: FG02-88ER13968

OSTI ID:: 1259320

Alternate ID(s):: OSTI ID: 1250268; OSTI ID: 1344491

Journal Information:: Molecular Cell, Journal Name: Molecular Cell Vol. 58 Journal Issue: 6; ISSN 1097-2765

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 272 works

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