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Title: Chapter 3: Isolation of Photosystem II Reaction Center Complexes from Plants

Book · · Methods in Molecular Biology
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Methods to isolate and purify 6- and 5-Chl D1/D2/Cyt b559 photosystem II (PSII) reaction center (RC) complexes from plants are presented, and the advantages and disadvantages of each procedure are discussed. One of the simpler 6-Chl procedures and a procedure for isolating 5-Chl complexes are described in detail. Furthermore, a rapid procedure that produces relatively large amounts of less pure 6-Chl material (i.e., more nonpigmented protein) is also described. Criteria to assess the purity of PSII RC preparations are presented, and problems associated with each of the isolation procedures are discussed.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC36-08GO28308
OSTI ID:
1258338
Report Number(s):
NREL/CH-2A00-52958
Journal Information:
Methods in Molecular Biology, Vol. 684; ISSN 1064-3745
Publisher:
Springer
Country of Publication:
United States
Language:
English

References (16)

Isolation of a photosystem II reaction center consisting of D-1 and D-2 polypeptides and cytochrome b-559 journal January 1987
Structure of the protein subunits in the photosynthetic reaction centre of Rhodopseudomonas viridis at 3Å resolution journal December 1985
S TRUCTURE , D YNAMICS , AND E NERGETICS OF THE P RIMARY P HOTOCHEMISTRY OF P HOTOSYSTEM II OF O XYGENIC P HOTOSYNTHESIS journal June 2002
Crystal structure of oxygen-evolving photosystem II from Thermosynechococcus vulcanus at 3.7-A resolution journal December 2002
Cyanobacterial photosystem II at 2.9-Å resolution and the role of quinones, lipids, channels and chloride journal February 2009
A highly resolved, oxygen-evolving photosystem II preparation from spinach thylakoid membranes: EPR and electron-transport properties journal November 1981
Inactivation of Photosynthetic Oxygen Evolution and Concomitant Release of Three Polypeptides in the Photosystem II Particles of Spinach Chloroplasts journal April 1982
Release of polypeptides from highly active O 2 -evolving photosystem-2 preparation by this treatment journal October 1981
Structural, biochemical and biophysical characterization of four oxygen-evolving Photosystem II preparations from spinach journal February 1984
Spectral, Photophysical, and Stability Properties of Isolated Photosystem II Reaction Center journal June 1988
Electron-transport properties of the isolated D1-D2-cytochrome b-559 Photosystem II reaction centre journal May 1988
Stabilization of Isolated Photosystem II Reaction Center Complex in the Dark and in the Light Using Polyethylene Glycol and an Oxygen-Scrubbing System journal February 1989
Photochemistry and spectroscopy of a five-chlorophyll reaction center of photosystem II isolated by using a Cu affinity column. journal March 1995
A routine method to determine the chlorophyll a, pheophytin a and β-carotene contents of isolated Photosystem II reaction center complexes journal April 1997
Charge Recombination Reactions in Photosystem II. 2. Transient Absorbance Difference Spectra and Their Temperature Dependence journal April 1995
Magneto-Optical Measurements of the Pigments in Fully Active Photosystem II Core Complexes from Plants † journal February 2002

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Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
intrinsic protein
isolatoin
pigment-protien complex
photosystem II
reaction center
purification
chromatography