A reversible Renilla luciferase protein complementation assay for rapid identification of protein-protein interactions reveals the existence of an interaction network involved in xyloglucan biosynthesis in the plant Golgi apparatus

Lund, C. H.; Bromley, J. R.; Stenbaek, A.; Rasmussen, R. E.; Scheller, H. V.; Sakuragi, Y.

doi:10.1093/jxb/eru401

Title: A reversible Renilla luciferase protein complementation assay for rapid identification of protein-protein interactions reveals the existence of an interaction network involved in xyloglucan biosynthesis in the plant Golgi apparatus

Journal Article · Sat Oct 18 00:00:00 EDT 2014 · Journal of Experimental Botany

DOI:https://doi.org/10.1093/jxb/eru401· OSTI ID:1257372

Lund, C. H. ^[1]; Bromley, J. R. ^[2]; Stenbaek, A. ^[1]; Rasmussen, R. E. ^[1]; Scheller, H. V. ^[3]; Sakuragi, Y. ^[1]

Univ. of Copenhagen (Denmark).
Univ. of Copenhagen (Denmark).; Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)

A growing body of evidence suggests that protein-protein interactions (PPIs) occur amongst glycosyltransferases (GTs) required for plant glycan biosynthesis (e.g. cell wall polysaccharides and N-glycans) in the Golgi apparatus, and may control the functions of these enzymes. However, identification of PPIs in the endomembrane system in a relatively fast and simple fashion is technically challenging, hampering the progress in understanding the functional coordination of the enzymes in Golgi glycan biosynthesis. To solve the challenges, we adapted and streamlined a reversible Renilla luciferase protein complementation assay (Rluc-PCA), originally reported for use in human cells, for transient expression in Nicotiana benthamiana. We tested Rluc-PCA and successfully identified luminescence complementation amongst Golgi-localizing GTs known to form a heterodimer (GAUT1 and GAUT7) and those which homooligomerize (ARAD1). In contrast, no interaction was shown between negative controls (e.g. GAUT7, ARAD1, IRX9). Rluc-PCA was used to investigate PPIs amongst Golgi-localizing GTs involved in biosynthesis of hemicelluloses. Although no PPI was identified among six GTs involved in xylan biosynthesis, Rluc-PCA confirmed three previously proposed interactions and identified seven novel PPIs amongst GTs involved in xyloglucan biosynthesis. Notably, three of the novel PPIs were confirmed by a yeast-based split-ubiquitin assay. Finally, Gateway-enabled expression vectors were generated, allowing rapid construction of fusion proteins to the Rluc reporters and epitope tags. Our results show that Rluc-PCA coupled with transient expression in N. benthamiana is a fast and versatile method suitable for analysis of PPIs between Golgi resident proteins in an easy and mid-throughput fashion in planta.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1257372

Alternate ID(s):: OSTI ID: 1511420

Journal Information:: Journal of Experimental Botany, Vol. 66, Issue 1; ISSN 0022-0957

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 26 works

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References (67)

New GATEWAY vectors for High Throughput Analyses of Protein–Protein Interactions by Bimolecular Fluorescence Complementation Gehl, Christian; Waadt, Rainer; Kudla, Jörg Molecular Plant, Vol. 2, Issue 5 https://doi.org/10.1093/mp/ssp040	journal	September 2009
Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments Nour-Eldin, Hussam H.; Hansen, Bjarne G.; Nørholm, Morten H. H. Nucleic Acids Research, Vol. 34, Issue 18 https://doi.org/10.1093/nar/gkl635	journal	September 2006
Cell-wall carbohydrates and their modification as a resource for biofuels Pauly, Markus; Keegstra, Kenneth The Plant Journal, Vol. 54, Issue 4, p. 559-568 https://doi.org/10.1111/j.1365-313X.2008.03463.x	journal	May 2008
RNA-Seq Analysis of Developing Nasturtium Seeds (Tropaeolum majus): Identification and Characterization of an Additional Galactosyltransferase Involved in Xyloglucan Biosynthesis Jensen, Jacob K.; Schultink, Alex; Keegstra, Kenneth Molecular Plant, Vol. 5, Issue 5 https://doi.org/10.1093/mp/sss032	journal	September 2012
A gene from the cellulose synthase-like C family encodes a -1,4 glucan synthase Cocuron, J. -C.; Lerouxel, O.; Drakakaki, G. Proceedings of the National Academy of Sciences, Vol. 104, Issue 20 https://doi.org/10.1073/pnas.0703133104	journal	May 2007
Monitoring protein conformations and interactions by fluorescence resonance energy transfer between mutants of green fluorescent protein Miyawaki, Atsushi; Tsien, Roger Y. Methods in Enzymology https://doi.org/10.1016/S0076-6879(00)27297-2	book	January 2000
Interactions between membrane-bound cellulose synthases involved in the synthesis of the secondary cell wall Timmers, Jaap; Vernhettes, Samantha; Desprez, Thierry FEBS Letters, Vol. 583, Issue 6 https://doi.org/10.1016/j.febslet.2009.02.035	journal	March 2009
The plant glycosyltransferase clone collection for functional genomics Lao, Jeemeng; Oikawa, Ai; Bromley, Jennifer R. The Plant Journal, Vol. 79, Issue 3 https://doi.org/10.1111/tpj.12577	journal	July 2014
The roles of enzyme localisation and complex formation in glycan assembly within the Golgi apparatus de Graffenried, Christopher L.; Bertozzi, Carolyn R. Current Opinion in Cell Biology, Vol. 16, Issue 4 https://doi.org/10.1016/j.ceb.2004.06.007	journal	August 2004
Arabidopsis irregular xylem8 and irregular xylem9 : Implications for the Complexity of Glucuronoxylan Biosynthesis Peña, Maria J.; Zhong, Ruiqin; Zhou, Gong-Ke The Plant Cell, Vol. 19, Issue 2 https://doi.org/10.1105/tpc.106.049320	journal	February 2007
Arabidopsis XXT5 gene encodes a putative α-1,6-xylosyltransferase that is involved in xyloglucan biosynthesis Zabotina, Olga A.; van de Ven, Wilhelmina T. G.; Freshour, Glenn The Plant Journal, Vol. 56, Issue 1 https://doi.org/10.1111/j.1365-313X.2008.03580.x	journal	October 2008
The Cytoskeleton and Gravitropism in Higher Plants Blancaflor, Elison B. Journal of Plant Growth Regulation, Vol. 21, Issue 2 https://doi.org/10.1007/s003440010041	journal	June 2002
Assessment of the integral membrane protein topology in living cells Zamyatnin, Andrey A.; Solovyev, Andrey G.; Bozhkov, Peter V. The Plant Journal, Vol. 46, Issue 1 https://doi.org/10.1111/j.1365-313X.2006.02674.x	journal	April 2006
Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with alpha -1,6-mannosyltransferase activity Jungmann, J. The EMBO Journal, Vol. 17, Issue 2 https://doi.org/10.1093/emboj/17.2.423	journal	January 1998
The MUR3 Gene of Arabidopsis Encodes a Xyloglucan Galactosyltransferase That Is Evolutionarily Related to Animal Exostosins Madson, Michael; Dunand, Christophe; Li, Xuemei The Plant Cell, Vol. 15, Issue 7 https://doi.org/10.1105/tpc.009837	journal	June 2003
The Interconversion of UDP-Arabinopyranose and UDP-Arabinofuranose Is Indispensable for Plant Development in Arabidopsis Rautengarten, Carsten; Ebert, Berit; Herter, Thomas The Plant Cell, Vol. 23, Issue 4 https://doi.org/10.1105/tpc.111.083931	journal	April 2011
The putative tumor suppressors EXT1 and EXT2 form a stable complex that accumulates in the Golgi apparatus and catalyzes the synthesis of heparan sulfate McCormick, C.; Duncan, G.; Goutsos, K. T. Proceedings of the National Academy of Sciences, Vol. 97, Issue 2 https://doi.org/10.1073/pnas.97.2.668	journal	January 2000
Comparison of five xylan synthesis mutants reveals new insight into the mechanisms of xylan synthesis Brown, David M.; Goubet, Florence; Wong, Vicky W. The Plant Journal, Vol. 52, Issue 6 https://doi.org/10.1111/j.1365-313X.2007.03307.x	journal	December 2007
The Arabidopsis RHD3 gene is required for cell wall biosynthesis and actin organization Hu, Yun; Zhong, Ruiqin; Morrison�III, W. Herbert Planta, Vol. 217, Issue 6 https://doi.org/10.1007/s00425-003-1067-7	journal	October 2003
TECHNICAL ADVANCE: Split luciferase complementation assay to study protein-protein interactions in Arabidopsis protoplasts: Protein-protein interactions in living plant cells Fujikawa, Yukichi; Kato, Naohiro The Plant Journal, Vol. 52, Issue 1 https://doi.org/10.1111/j.1365-313X.2007.03214.x	journal	July 2007
Xylan Biosynthesis in Plants, Simply Complex Faik, Ahmed; Jiang, Nan; Held, Michael A. Plants and BioEnergy https://doi.org/10.1007/978-1-4614-9329-7_10	book	November 2013
USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products Geu-Flores, F.; Nour-Eldin, H. H.; Nielsen, M. T. Nucleic Acids Research, Vol. 35, Issue 7 https://doi.org/10.1093/nar/gkm106	journal	March 2007
Evidence for Network Evolution in an Arabidopsis Interactome Map Dreze, M.; Carvunis, A. -R.; Charloteaux, B. Science, Vol. 333, Issue 6042 https://doi.org/10.1126/science.1203877	journal	July 2011
Visual Mapping of Cell Wall Biosynthesis Sakuragi, Yumiko; Nørholm, Morten H. H.; Scheller, Henrik V. Methods in Molecular Biology https://doi.org/10.1007/978-1-61779-008-9_11	book	December 2010
Mutations in Multiple XXT Genes of Arabidopsis Reveal the Complexity of Xyloglucan Biosynthesis Zabotina, Olga A.; Avci, Utku; Cavalier, David Plant Physiology, Vol. 159, Issue 4 https://doi.org/10.1104/pp.112.198119	journal	June 2012
Localization of Golgi-resident glycosyltransferases Tu, Linna; Banfield, David Karl Cellular and Molecular Life Sciences, Vol. 67, Issue 1 https://doi.org/10.1007/s00018-009-0126-z	journal	September 2009
Arabidopsis mannan synthase CSLA9 and glucan synthase CSLC4 have opposite orientations in the Golgi membrane: Hemicellulosic glycan synthase topology Davis, Jonathan; Brandizzi, Federica; Liepman, Aaron H. The Plant Journal, Vol. 64, Issue 6 https://doi.org/10.1111/j.1365-313X.2010.04392.x	journal	November 2010
Border Control--A Membrane-Linked Interactome of Arabidopsis Jones, A. M.; Xuan, Y.; Xu, M. Science, Vol. 344, Issue 6185 https://doi.org/10.1126/science.1251358	journal	May 2014
K+ channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions Obrdlik, P.; El-Bakkoury, M.; Hamacher, T. Proceedings of the National Academy of Sciences, Vol. 101, Issue 33 https://doi.org/10.1073/pnas.0404467101	journal	August 2004
Disrupting Two Arabidopsis thaliana Xylosyltransferase Genes Results in Plants Deficient in Xyloglucan, a Major Primary Cell Wall Component Cavalier, David M.; Lerouxel, Olivier; Neumetzler, Lutz The Plant Cell, Vol. 20, Issue 6 https://doi.org/10.1105/tpc.108.059873	journal	June 2008
A multicolored set of in vivo organelle markers for co-localization studies in Arabidopsis and other plants: Fluorescent organelle markers Nelson, Brook K.; Cai, Xue; Nebenführ, Andreas The Plant Journal, Vol. 51, Issue 6 https://doi.org/10.1111/j.1365-313X.2007.03212.x	journal	August 2007
Biochemical Characterization and Molecular Cloning of an α-1,2-Fucosyltransferase That Catalyzes the Last Step of Cell Wall Xyloglucan Biosynthesis in Pea Faik, Ahmed; Bar-Peled, Maor; DeRocher, Amy E. Journal of Biological Chemistry, Vol. 275, Issue 20 https://doi.org/10.1074/jbc.M000677200	journal	March 2000
Quantitative analysis of dynamic protein-protein interactions in planta by a floated-leaf luciferase complementation imaging (FLuCI) assay using binary Gateway vectors: Floated-leaf luciferase complementation imaging assay Gehl, Christian; Kaufholdt, David; Hamisch, Domenica The Plant Journal, Vol. 67, Issue 3 https://doi.org/10.1111/j.1365-313X.2011.04607.x	journal	June 2011
The Split Luciferase Complementation Assay Kato, Naohiro; Jones, Jason Plant Developmental Biology https://doi.org/10.1007/978-1-60761-765-5_24	book	January 2010
Xyloglucan Fucosyltransferase, an Enzyme Involved in Plant Cell Wall Biosynthesis Perrin, R. M. Science, Vol. 284, Issue 5422 https://doi.org/10.1126/science.284.5422.1976	journal	June 1999
Homogalacturonan Methyl-Esterification and Plant Development Wolf, Sebastian; Mouille, Grégory; Pelloux, Jérome Molecular Plant, Vol. 2, Issue 5 https://doi.org/10.1093/mp/ssp066	journal	September 2009
Split ubiquitin as a sensor of protein interactions in vivo. Johnsson, N.; Varshavsky, A. Proceedings of the National Academy of Sciences, Vol. 91, Issue 22 https://doi.org/10.1073/pnas.91.22.10340	journal	October 1994
Galacturonosyltransferase (GAUT)1 and GAUT7 are the core of a plant cell wall pectin biosynthetic homogalacturonan:galacturonosyltransferase complex Atmodjo, M. A.; Sakuragi, Y.; Zhu, X. Proceedings of the National Academy of Sciences, Vol. 108, Issue 50 https://doi.org/10.1073/pnas.1112816108	journal	November 2011
A Glucurono(arabino)xylan Synthase Complex from Wheat Contains Members of the GT43, GT47, and GT75 Families and Functions Cooperatively Zeng, Wei; Jiang, Nan; Nadella, Ramya Plant Physiology, Vol. 154, Issue 1 https://doi.org/10.1104/pp.110.159749	journal	July 2010
Predicting transmembrane protein topology with a hidden markov model: application to complete genomes11Edited by F. Cohen Krogh, Anders; Larsson, Björn; von Heijne, Gunnar Journal of Molecular Biology, Vol. 305, Issue 3 https://doi.org/10.1006/jmbi.2000.4315	journal	January 2001
Analysis of Xyloglucan Fucosylation in Arabidopsis Perrin, Robyn M.; Jia, Zhonghua; Wagner, Tanya A. Plant Physiology, Vol. 132, Issue 2 https://doi.org/10.1104/pp.102.016642	journal	April 2003
Golgi N -Glycosyltransferases Form Both Homo- and Heterodimeric Enzyme Complexes in Live Cells Hassinen, Antti; Rivinoja, Antti; Kauppila, Annika Journal of Biological Chemistry, Vol. 285, Issue 23 https://doi.org/10.1074/jbc.M110.103184	journal	April 2010
Progress in the biological synthesis of the plant cell wall: new ideas for improving biomass for bioenergy Carpita, Nicholas C. Current Opinion in Biotechnology, Vol. 23, Issue 3 https://doi.org/10.1016/j.copbio.2011.12.003	journal	June 2012
Golgi-localized enzyme complexes for plant cell wall biosynthesis Oikawa, Ai; Lund, Christian Have; Sakuragi, Yumiko Trends in Plant Science, Vol. 18, Issue 1 https://doi.org/10.1016/j.tplants.2012.07.002	journal	January 2013
Time-Resolved Fluorescence Imaging Reveals Differential Interactions of N -Glycan Processing Enzymes across the Golgi Stack in Planta Schoberer, Jennifer; Liebminger, Eva; Botchway, Stanley W. Plant Physiology, Vol. 161, Issue 4 https://doi.org/10.1104/pp.112.210757	journal	February 2013
A genetic system based on split-ubiquitin for the analysis of interactions between membrane proteins in vivo Stagljar, I.; Korostensky, C.; Johnsson, N. Proceedings of the National Academy of Sciences, Vol. 95, Issue 9 https://doi.org/10.1073/pnas.95.9.5187	journal	April 1998
Characterization of IRX10 and IRX10-like reveals an essential role in glucuronoxylan biosynthesis in Arabidopsis Brown, David M.; Zhang, Zhinong; Stephens, Elaine The Plant Journal, Vol. 57, Issue 4 https://doi.org/10.1111/j.1365-313X.2008.03729.x	journal	February 2009
Quantification of dynamic protein complexes using Renilla luciferase fragment complementation applied to protein kinase A activities in vivo Stefan, E.; Aquin, S.; Berger, N. Proceedings of the National Academy of Sciences, Vol. 104, Issue 43 https://doi.org/10.1073/pnas.0704257104	journal	October 2007
GO-PROMTO Illuminates Protein Membrane Topologies of Glycan Biosynthetic Enzymes in the Golgi Apparatus of Living Tissues Søgaard, Casper; Stenbæk, Anne; Bernard, Sophie PLoS ONE, Vol. 7, Issue 2 https://doi.org/10.1371/journal.pone.0031324	journal	February 2012
Xyloglucan Xylosyltransferases XXT1, XXT2, and XXT5 and the Glucan Synthase CSLC4 Form Golgi-Localized Multiprotein Complexes Chou, Yi-Hsiang; Pogorelko, Gennady; Zabotina, Olga A. Plant Physiology, Vol. 159, Issue 4 https://doi.org/10.1104/pp.112.199356	journal	June 2012
Gateway-compatible vectors for plant functional genomics and proteomics Earley, Keith W.; Haag, Jeremy R.; Pontes, Olga The Plant Journal, Vol. 45, Issue 4 https://doi.org/10.1111/j.1365-313X.2005.02617.x	journal	February 2006
Stacks on tracks: the plant Golgi apparatus traffics on an actin/ER network ^† Boevink, Petra; Oparka, Karl; Cruz, Simon Santa The Plant Journal, Vol. 15, Issue 3 https://doi.org/10.1046/j.1365-313X.1998.00208.x	journal	August 1998
Retracted: An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus Voinnet, Olivier; Rivas, Susana; Mestre, Pere The Plant Journal, Vol. 33, Issue 5 https://doi.org/10.1046/j.1365-313X.2003.01676.x	journal	February 2003
An in vivo, luciferase-based, Agrobacterium-infiltration assay system: implications for post-transcriptional gene silencing Cazzonelli, Christopher Ian; Velten, Jeff Planta, Vol. 224, Issue 3 https://doi.org/10.1007/s00425-006-0250-z	journal	March 2006
KATAMARI1/MURUS3 Is a Novel Golgi Membrane Protein That Is Required for Endomembrane Organization in Arabidopsis Tamura, Kentaro; Shimada, Tomoo; Kondo, Maki The Plant Cell, Vol. 17, Issue 6 https://doi.org/10.1105/tpc.105.031930	journal	April 2005
ARAMEMNON, a Novel Database for Arabidopsis Integral Membrane Proteins Schwacke, Rainer; Schneider, Anja; van der Graaff, Eric Plant Physiology, Vol. 131, Issue 1 https://doi.org/10.1104/pp.011577	journal	January 2003
Two Xyloglucan Xylosyltransferases Catalyze the Addition of Multiple Xylosyl Residues to Cellohexaose Cavalier, David M.; Keegstra, Kenneth Journal of Biological Chemistry, Vol. 281, Issue 45 https://doi.org/10.1074/jbc.M606379200	journal	September 2006
Detecting Protein−Protein Interactions with a Green Fluorescent Protein Fragment Reassembly Trap: Scope and Mechanism Magliery, Thomas J.; Wilson, Christopher G. M.; Pan, Weilan Journal of the American Chemical Society, Vol. 127, Issue 1 https://doi.org/10.1021/ja046699g	journal	January 2005
New methodologies for measuring protein interactions in vivo and in vitro Piehler, Jacob Current Opinion in Structural Biology, Vol. 15, Issue 1 https://doi.org/10.1016/j.sbi.2005.01.008	journal	February 2005
ARABINAN DEFICIENT 1 Is a Putative Arabinosyltransferase Involved in Biosynthesis of Pectic Arabinan in Arabidopsis Harholt, Jesper; Jensen, Jacob Krüger; Sørensen, Susanne Oxenbøll Plant Physiology, Vol. 140, Issue 1 https://doi.org/10.1104/pp.105.072744	journal	December 2005
A plant mutase that interconverts UDP-arabinofuranose and UDP-arabinopyranose Konishi, Teruko; Takeda, Takumi; Miyazaki, Yasumasa Glycobiology, Vol. 17, Issue 3 https://doi.org/10.1093/glycob/cwl081	journal	December 2006
Kin recognition: A model for the retention of Golgi enzymes Nilsson, Tommy; Slusarewicz, Paul; Hoe, Mee H. FEBS Letters, Vol. 330, Issue 1 https://doi.org/10.1016/0014-5793(93)80906-b	journal	September 1993
The plant Golgi apparatus Dupree, Paul; Sherrier, D. Janine Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol. 1404, Issue 1-2 https://doi.org/10.1016/s0167-4889(98)00061-5	journal	August 1998
A suite of tools and application notes for in vivo protein interaction assays using bioluminescence resonance energy transfer (BRET) Subramanian, Chitra; Woo, Jongchan; Cai, Xue The Plant Journal, Vol. 48, Issue 1 https://doi.org/10.1111/j.1365-313x.2006.02851.x	journal	October 2006
Golgi-Mediated Synthesis and Secretion of Matrix Polysaccharides of the Primary Cell Wall of Higher Plants Driouich, Azeddine; Follet-Gueye, Marie-Laure; Bernard, Sophie Frontiers in Plant Science, Vol. 3 https://doi.org/10.3389/fpls.2012.00079	journal	January 2012
Xyloglucan and Its Biosynthesis Zabotina, Olga A. Frontiers in Plant Science, Vol. 3 https://doi.org/10.3389/fpls.2012.00134	journal	January 2012
Plant cell wall dynamics and wall-related susceptibility in plantâ€“pathogen interactions Bellincampi, Daniela; Cervone, Felice; Lionetti, Vincenzo Frontiers in Plant Science, Vol. 5 https://doi.org/10.3389/fpls.2014.00228	journal	May 2014

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59 BASIC BIOLOGICAL SCIENCES
Arabidopsis thaliana
glycosyltransferase
Golgi apparatus
Nicotiana benthamiana
plant cell wall
polysaccharides
protein–protein interaction
Renilla luciferase
type II membrane protein
xyloglucan