Cloning, expression, and regulation of tissue-specific genes in Drosophila
- Institute of Gene Biology, Moscow (Russian Federation)
The family of esterase genes was studied in various Drosophilia species. These genes are classified as tissue-specific and housekeeping ones. The expression of tissue-specific esterases in the male reproductive system of Drosophilia species from the virilis and melanogaster groups was thoroughly examined. Modifier genes controlling activity level, time of synthesis, and distribution in cells of the tissue-specific esterase isozyme from the ejaculatory bulb were revealed. The structural gene coding of this enzyme was isolated, cloned, and sequenced. This gene was shown to be similar in different Drosophilia species; the transcriptional level of tissue specificity of this gene was determined. The possibility of transformating the tissue-specific gene into a housekeeping one was demonstrated. In different Drosophilia species, this gene can be expressed in different parts of the reproductive system. In transgenic males carrying the gene of another species, the foreign gene is expressed as in the donor. 68 refs., 11 figs.
- OSTI ID:
- 125445
- Journal Information:
- Russian Journal of Genetics, Vol. 31, Issue 8; Other Information: PBD: Aug 1995; TN: Translated from Genetika; 31: No. 8, 1029-1042(1995)
- Country of Publication:
- United States
- Language:
- English
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