Structure of a bacterial virus DNA-injection protein complex reveals a decameric assembly with a constricted molecular channel

Zhao, Haiyan; Speir, Jeffrey A.; Matsui, Tsutomu; Lin, Zihan; Liang, Lingfei; Lynn, Anna Y.; Varnado, Brittany; Weiss, Thomas M.; Tang, Liang; Schuch, Raymond

doi:10.1371/journal.pone.0149337

Structure of a bacterial virus DNA-injection protein complex reveals a decameric assembly with a constricted molecular channel

Journal Article · Tue Feb 16 00:00:00 EST 2016 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0149337· OSTI ID:1253628

Zhao, Haiyan ^[1]; Speir, Jeffrey A. ^[2]; Matsui, Tsutomu ^[3]; Lin, Zihan ^[1]; Liang, Lingfei ^[1]; Lynn, Anna Y. ^[1]; Varnado, Brittany ^[1]; Weiss, Thomas M. ^[3]; Tang, Liang ^[1]; Schuch, Raymond ^[4]

Univ. of Kansas, Lawrence, KS (United States)
The Scripps Research Institute, La Jolla, CA (United States)
SLAC National Accelerator Lab., Menlo Park, CA (United States)
ContraFect Corp., Yonkers, NY (United States)

The multi-layered cell envelope structure of Gram-negative bacteria represents significant physical and chemical barriers for short-tailed phages to inject phage DNA into the host cytoplasm. Here we show that a DNA-injection protein of bacteriophage Sf6, gp12, forms a 465-kDa, decameric assembly in vitro. The electron microscopic structure of the gp12 assembly shows a ~150-Å, mushroom-like architecture consisting of a crown domain and a tube-like domain, which embraces a 25-Å-wide channel that could precisely accommodate dsDNA. The constricted channel suggests that gp12 mediates rapid, uni-directional injection of phage DNA into host cells by providing a molecular conduit for DNA translocation. The assembly exhibits a 10-fold symmetry, which may be a common feature among DNA-injection proteins of P22-like phages and may suggest a symmetry mismatch with respect to the 6-fold symmetric phage tail. As a result, the gp12 monomer is highly flexible in solution, supporting a mechanism for translocation of the protein through the conduit of the phage tail toward the host cell envelope, where it assembles into a DNA-injection device.

View Accepted Manuscript (DOE)

Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1253628

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 2 Vol. 11; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
DNA structure
bacteriophages
cell membranes
host cells
molecular structure
negative staining
protein structure
viral packaging

Structure of a bacterial virus DNA-injection protein complex reveals a decameric assembly with a constricted molecular channel

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