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Title: X-ray absorption spectroscopy and EPR studies of oriented spinach thylakoid preparations

Abstract

In this study, oriented Photosystem II (PS II) particles from spinach chloroplasts are studied with electron paramagnetic resonance (EPR) and x-ray absorption spectroscopy (XAS) to determine more details of the structure of the oxygen evolving complex (OEC). The nature of halide binding to Mn is also studied with Cl K-edge and Mn EXAFS (extended x-ray absorption fine structure) of Mn-Cl model compounds, and with Mn EXAFS of oriented PS II in which Br has replaced Cl. Attention is focused on the following: photosynthesis and the oxygen evolving complex; determination of mosaic spread in oriented photosystem II particles from signal II EPR measurement; oriented EXAFS--studies of PS II in the S{sub 2} state; structural changes in PS II as a result of treatment with ammonia: EPR and XAS studies; studies of halide binding to Mn: Cl K-edge and Mn EXAFS of Mn-Cl model compounds and Mn EXAFS of oriented Br-treated photosystem II.

Authors:
 [1]
  1. Univ. of California, Berkeley, CA (United States). Dept. of Chemistry|[Lawrence Berkeley Lab., CA (United States). Structural Biology Div.
Publication Date:
Research Org.:
Lawrence Berkeley Lab., CA (United States)
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
125043
Report Number(s):
LBL-37695
ON: DE96002060; TRN: AHC29528%%78
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Technical Report
Resource Relation:
Other Information: TH: Thesis (Ph.D.); PBD: Aug 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; PHOTOSYNTHETIC REACTION CENTERS; STRUCTURAL CHEMICAL ANALYSIS; SPINACH; X-RAY SPECTROSCOPY; ELECTRON SPIN RESONANCE; BIOCHEMICAL REACTION KINETICS; EXPERIMENTAL DATA

Citation Formats

Andrews, J.C. X-ray absorption spectroscopy and EPR studies of oriented spinach thylakoid preparations. United States: N. p., 1995. Web. doi:10.2172/125043.
Andrews, J.C. X-ray absorption spectroscopy and EPR studies of oriented spinach thylakoid preparations. United States. doi:10.2172/125043.
Andrews, J.C. Tue . "X-ray absorption spectroscopy and EPR studies of oriented spinach thylakoid preparations". United States. doi:10.2172/125043. https://www.osti.gov/servlets/purl/125043.
@article{osti_125043,
title = {X-ray absorption spectroscopy and EPR studies of oriented spinach thylakoid preparations},
author = {Andrews, J.C.},
abstractNote = {In this study, oriented Photosystem II (PS II) particles from spinach chloroplasts are studied with electron paramagnetic resonance (EPR) and x-ray absorption spectroscopy (XAS) to determine more details of the structure of the oxygen evolving complex (OEC). The nature of halide binding to Mn is also studied with Cl K-edge and Mn EXAFS (extended x-ray absorption fine structure) of Mn-Cl model compounds, and with Mn EXAFS of oriented PS II in which Br has replaced Cl. Attention is focused on the following: photosynthesis and the oxygen evolving complex; determination of mosaic spread in oriented photosystem II particles from signal II EPR measurement; oriented EXAFS--studies of PS II in the S{sub 2} state; structural changes in PS II as a result of treatment with ammonia: EPR and XAS studies; studies of halide binding to Mn: Cl K-edge and Mn EXAFS of Mn-Cl model compounds and Mn EXAFS of oriented Br-treated photosystem II.},
doi = {10.2172/125043},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Aug 01 00:00:00 EDT 1995},
month = {Tue Aug 01 00:00:00 EDT 1995}
}

Technical Report:

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