Combining treatments to improve the fermentation of sugarcane bagasse hydrolysates by ethanologenic Escherichia coli LY180

Geddes, Ryan; Shanmugam, Keelnatham T.; Ingram, Lonnie O.

doi:10.1016/j.biortech.2015.03.141

Title: Combining treatments to improve the fermentation of sugarcane bagasse hydrolysates by ethanologenic Escherichia coli LY180

Journal Article · Tue Mar 31 00:00:00 EDT 2015 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2015.03.141· OSTI ID:1771948

Geddes, Ryan ^[1]; Shanmugam, Keelnatham T. ^[1]; Ingram, Lonnie O. ^[1]

Univ. of Florida, Gainesville, FL (United States)

Inhibitory side products from dilute acid pretreatment is a major challenge for conversion of lignocellulose into ethanol. Six strategies to detoxify sugarcane hydrolysates were investigated alone, and in combinations (vacuum evaporation of volatiles, high pH treatment with ammonia, laccase, bisulfite, microaeration, and inoculum size). High pH was the most beneficial single treatment, increasing the minimum inhibitory concentration (measured by ethanol production) from 15% (control) to 70% hydrolysate. Combining treatments provided incremental improvements, consistent with different modes of action and multiple inhibitory compounds. Screening toxicity using tube cultures proved to be an excellent predictor of relative performance in pH-controlled fermenters. Here, a combination of treatments (vacuum evaporation, laccase, high pH, bisulfite, microaeration) completely eliminated all inhibitory activity present in hydrolysate. With this combination, fermentation of hemicellulose sugars (90% hydrolysate) to ethanol was complete within 48 h, identical to the fermentation of laboratory xylose (50 g/L) in AM1 mineral salts medium (without hydrolysate).

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Research Organization:: Univ. of Florida, Gainesville, FL (United States)

Sponsoring Organization:: USDOE Office of International Affairs (IA)

Grant/Contract Number:: PI0000031

OSTI ID:: 1771948

Alternate ID(s):: OSTI ID: 1250369

Journal Information:: Bioresource Technology, Vol. 189; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 13 works

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Chromosomal mutations in Escherichia coli that improve tolerance to nonvolatile side‐products from dilute acid treatment of sugarcane bagasse Shi, Aiqin; Yomano, Lorraine P.; York, Sean W. Biotechnology and Bioengineering, Vol. 117, Issue 1 https://doi.org/10.1002/bit.27189	journal	November 2019
Techno-Economic Evaluation of Cellulosic Ethanol Production Based on Pilot Biorefinery Data: a Case Study of Sweet Sorghum Bagasse Processed via L+SScF van Rijn, Rick; Nieves, Ismael U.; Shanmugam, K. T. BioEnergy Research, Vol. 11, Issue 2 https://doi.org/10.1007/s12155-018-9906-3	journal	April 2018
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59 BASIC BIOLOGICAL SCIENCES
Hemicellulose
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Ethanol
Furfural
Inhibitors

Title: Combining treatments to improve the fermentation of sugarcane bagasse hydrolysates by ethanologenic Escherichia coli LY180

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