Crystal structure of the protein At3g01520, a eukaryotic universal stress protein-like protein from arabidopsis thaliana in complex with AMP

Kim, Do Jin; Bitto, Eduard; Bingman, Craig A.; Kim, Hyun-Jung; Han, Byung Woo; Phillips, Jr., George N.

doi:10.1002/prot.24821

Crystal structure of the protein At3g01520, a eukaryotic universal stress protein-like protein from arabidopsis thaliana in complex with AMP

Journal Article · Tue Apr 28 00:00:00 EDT 2015 · Proteins

DOI:https://doi.org/10.1002/prot.24821· OSTI ID:1247327

Kim, Do Jin ^[1]; Bitto, Eduard ^[2]; Bingman, Craig A. ^[3]; Kim, Hyun-Jung ^[4]; Han, Byung Woo ^[1]; Phillips, Jr., George N. ^[5]

Seoul National Univ. (Korea)
Georgian Court Univ., Lakewood, NJ (United States)
Univ. of Wisconsin, Madison WI (United States)
Chung-Ang Univ., Seoul (Korea)
Univ. of Wisconsin, Madison, WI (United States); Rice Univ., Houston, TX (United States)

Members of the universal stress protein (USP) family are conserved in a phylogenetically diverse range of prokaryotes, fungi, protists, and plants and confer abilities to respond to a wide range of environmental stresses. Arabidopsis thaliana contains 44 USP domain–containing proteins, and USP domain is found either in a small protein with unknown physiological function or in an N–terminal portion of a multi–domain protein, usually a protein kinase. Here, we report the first crystal structure of a eukaryotic USP–like protein encoded from the gene At3g01520. The crystal structure of the protein At3g01520 was determined by the single–wavelength anomalous dispersion method and refined to an R factor of 21.8% (R_free = 26.1%) at 2.5 Å resolution. The crystal structure includes three At3g01520 protein dimers with one AMP molecule bound to each protomer, comprising a Rossmann–like α/β overall fold. The bound AMP and conservation of residues in the ATP–binding loop suggest that the protein At3g01520 also belongs to the ATP–binding USP subfamily members.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: Future Planning of Korea; ICT; Ministry of Science; National Inst. of Health; USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

OSTI ID:: 1247327

Journal Information:: Proteins, Journal Name: Proteins Journal Issue: 7 Vol. 83; ISSN 0887-3585

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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59 BASIC BIOLOGICAL SCIENCES
Arabidopsis thaliana
At3g01520
universal stress protein

Crystal structure of the protein At3g01520, a eukaryotic universal stress protein-like protein from arabidopsis thaliana in complex with AMP

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References (30)

Cited By (3)

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