An interface for the direct coupling of small liquid samples to AMS

Ognibene, T. J.; Thomas, A. T.; Daley, P. F.; Bench, G.; Turteltaub, K. W.

doi:10.1016/j.nimb.2015.05.018

An interface for the direct coupling of small liquid samples to AMS

Journal Article · Thu May 28 00:00:00 EDT 2015 · Nuclear Instruments and Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms

DOI:https://doi.org/10.1016/j.nimb.2015.05.018· OSTI ID:1266673

Ognibene, T. J. ^[1]; Thomas, A. T. ^[1]; Daley, P. F. ^[1]; Bench, G. ^[1]; Turteltaub, K. W. ^[1]

Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

We describe the moving wire interface attached to the 1-MV AMS system at LLNL’s Center for Accelerator Mass Spectrometry for the analysis of nonvolatile liquid samples as either discrete drops or from the direct output of biochemical separatory instrumentation, such as high-performance liquid chromatography (HPLC). Discrete samples containing at least a few 10 s of nanograms of carbon and as little as 50 zmol ¹⁴C can be measured with a 3–5% precision in a few minutes. The dynamic range of our system spans approximately 3 orders in magnitude. Sample to sample memory is minimized by the use of fresh targets for each discrete sample or by minimizing the amount of carbon present in a peak generated by an HPLC containing a significant amount of ¹⁴C. As a result, liquid sample AMS provides a new technology to expand our biomedical AMS program by enabling the capability to measure low-level biochemicals in extremely small samples that would otherwise be inaccessible.

Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1266673

Alternate ID(s):: OSTI ID: 22564579
OSTI ID: 1246215

Report Number(s):: LLNL-JRNL--664719

Journal Information:: Nuclear Instruments and Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms, Journal Name: Nuclear Instruments and Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms Journal Issue: C Vol. 361; ISSN 0168-583X

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

References (13)

High-sensitivity liquid chromatography-combustion isotope ratio mass spectrometry of fat-soluble vitamins Caimi, Richard J.; Brenna, J. Thomas Journal of Mass Spectrometry, Vol. 30, Issue 3 https://doi.org/10.1002/jms.1190300311	journal	March 1995
Interaction of titanium and vanadium with carbon dioxide in heating Vlasyuk, R. Z.; Kurovskii, V. Ya.; Lyapunov, A. P. Soviet Powder Metallurgy and Metal Ceramics, Vol. 25, Issue 1 https://doi.org/10.1007/BF00843021	journal	January 1986
Installation of hybrid ion source on the 1-MV LLNL BioAMS spectrometer Ognibene, T. J.; Salazar, G. A. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, Vol. 294 https://doi.org/10.1016/j.nimb.2011.11.029	journal	January 2013
Improving a gas ion source for 14C AMS Fahrni, S. M.; Wacker, L.; Synal, H. -A. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, Vol. 294 https://doi.org/10.1016/j.nimb.2012.03.037	journal	January 2013
Design of a secondary ionization target for direct production of a C− beam from CO2 pulses for online AMS Salazar, Gary; Ognibene, Ted Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, Vol. 294 https://doi.org/10.1016/j.nimb.2012.03.039	journal	January 2013
High-precision liquid chromatography-combustion isotope ratio mass spectrometry Caimi, Richard J.; Brenna, J. Thomas. Analytical Chemistry, Vol. 65, Issue 23 https://doi.org/10.1021/ac00071a028	journal	December 1993
A High-Throughput Method for the Conversion of CO ₂ Obtained from Biochemical Samples to Graphite in Septa-Sealed Vials for Quantification of ¹⁴ C via Accelerator Mass Spectrometry Ognibene, Ted J.; Bench, Graham; Vogel, John S. Analytical Chemistry, Vol. 75, Issue 9 https://doi.org/10.1021/ac026334j	journal	May 2003
Moving-Wire Device for Carbon Isotopic Analyses of Nanogram Quantities of Nonvolatile Organic Carbon Sessions, Alex L.; Sylva, Sean P.; Hayes, John M. Analytical Chemistry, Vol. 77, Issue 20 https://doi.org/10.1021/ac051251z	journal	October 2005
Ultrahigh Efficiency Moving Wire Combustion Interface for Online Coupling of High-Performance Liquid Chromatography (HPLC) Thomas, Avi T.; Ognibene, Ted; Daley, Paul Analytical Chemistry, Vol. 83, Issue 24 https://doi.org/10.1021/ac202013s	journal	December 2011
Directly Coupled High-Performance Liquid Chromatography–Accelerator Mass Spectrometry Measurement of Chemically Modified Protein and Peptides Thomas, Avi T.; Stewart, Benjamin J.; Ognibene, Ted J. Analytical Chemistry, Vol. 85, Issue 7 https://doi.org/10.1021/ac303609n	journal	March 2013
Gettering of Gas by Titanium Stout, Virgil L.; Gibbons, Martin D. Journal of Applied Physics, Vol. 26, Issue 12 https://doi.org/10.1063/1.1721936	journal	December 1955
Isotope-Ratio-Monitoring Liquid Chromatography Mass Spectrometry (IRM-LCMS): First Results from a Moving Wire Interface System Brand, W. A.; Dobberstein, P. Isotopes in Environmental and Health Studies, Vol. 32, Issue 2-3 https://doi.org/10.1080/10256019608036320	journal	August 1996
Carbon-isotopic analysis of microbial cells sorted by flow cytometry Eek, K. M.; Sessions, A. L.; Lies, D. P. Geobiology, Vol. 5, Issue 1 https://doi.org/10.1111/j.1472-4669.2006.00094.x	journal	March 2007

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Radiocarbon Tracers in Toxicology and Medicine: Recent Advances in Technology and Science Malfatti, Michael A.; Buchholz, Bruce A.; Enright, Heather A. Toxics, Vol. 7, Issue 2 https://doi.org/10.3390/toxics7020027	journal	May 2019

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An interface for the direct coupling of small liquid samples to AMS

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