Lipidomic and proteomic analysis of Caenorhabditis elegans lipid droplets and identification of ACS-4 as a lipid droplet-associated protein

Vrablik, Tracy L.; Petyuk, Vladislav A.; Larson, Emily M.; Smith, Richard D.; Watts, Jennifer

doi:10.1016/j.bbalip.2015.06.004

Title: Lipidomic and proteomic analysis of Caenorhabditis elegans lipid droplets and identification of ACS-4 as a lipid droplet-associated protein

Journal Article · Sat Jun 27 00:00:00 EDT 2015 · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids

DOI:https://doi.org/10.1016/j.bbalip.2015.06.004· OSTI ID:1243278

Vrablik, Tracy L. ^[1]; Petyuk, Vladislav A. ^[2]; Larson, Emily M. ^[1]; Smith, Richard D. ^[2]; Watts, Jennifer ^[1]

Washington State Univ., Pullman, WA (United States)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Lipid droplets are cytoplasmic organelles that store neutral lipids for membrane synthesis and energy reserves. In this study, we characterized the lipid and protein composition of purified C. elegans lipid droplets. These lipid droplets are composed mainly of triacylglycerols, surrounded by a phospholipid monolayer composed primarily of phosphatidylcholine and phosphatidylethanolamine. The fatty acid composition of the triacylglycerols was rich in fatty acid species obtained from the dietary E. coli, including cyclopropane fatty acids and cis-vaccenic acid. Unlike other organisms, C. elegans lipid droplets contain very little cholesterol or cholesterol esters. Comparison of the lipid droplet proteomes of wild type and high-fat daf-2 mutant strains shows a relative decrease of MDT-28 abundance in lipid droplets isolated from daf-2 mutants. Functional analysis of lipid droplet proteins identified in our proteomic studies indicated an enrichment of proteins required for growth and fat homeostasis in C. elegans.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1243278

Report Number(s):: PNNL-SA-108157; 48135; 400412000

Journal Information:: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol. 1851, Issue 10; ISSN 1388-1981

Country of Publication:: United States

Language:: English

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