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Quenching of Unincorporated Amplification Signal Reporters in Reverse-Transcription Loop-Mediated Isothermal Amplification Enabling Bright, Single-Step, Closed-Tube, and Multiplexed Detection of RNA Viruses

Journal Article · · Analytical Chemistry
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  1. Sandia National Laboratories, P.O. Box 969, MS 9291, Livermore, California 94551, United States
  2. University of California Davis, School of Veterinary Medicine, Department of Pathology, Microbiology and Immunology, One Shield Avenue, Davis, California 95616, United States

Not Available

Sponsoring Organization:
USDOE National Nuclear Security Administration (NNSA)
Grant/Contract Number:
AC04-94AL85000
OSTI ID:
1243142
Alternate ID(s):
OSTI ID: 1319449
Journal Information:
Analytical Chemistry, Journal Name: Analytical Chemistry Journal Issue: 7 Vol. 88; ISSN 0003-2700
Publisher:
American Chemical SocietyCopyright Statement
Country of Publication:
United States
Language:
English

References (22)

Detection of Loop-Mediated Isothermal Amplification Reaction by Turbidity Derived from Magnesium Pyrophosphate Formation journal November 2001
Development of a pan-serotype reverse transcription loop-mediated isothermal amplification assay for the detection of dengue virus journal September 2015
Rapid detection of HIV-1 by reverse-transcription, loop-mediated isothermal amplification (RT-LAMP) journal August 2008
Simultaneous detection and quantitation of Chikungunya, Dengue and West Nile viruses by multiplex RT-PCR assays and Dengue virus typing using High Resolution Melting journal December 2009
Homogenous, real-time duplex loop-mediated isothermal amplification using a single fluorophore-labeled primer and an intercalator dye: Its application to the simultaneous detection of Shiga toxin genes 1 and 2 in Shiga toxigenic Escherichia coli isolates journal August 2010
Development of a multiplex loop-mediated isothermal amplification (mLAMP) method for the simultaneous detection of bovine Babesia parasites journal December 2007
Mechanistic Evaluation of the Pros and Cons of Digital RT-LAMP for HIV-1 Viral Load Quantification on a Microfluidic Device and Improved Efficiency via a Two-Step Digital Protocol journal January 2013
Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products journal April 2008
Loop-mediated isothermal amplification of DNA journal June 2000
Molecular Zipper: a fluorescent probe for real-time isothermal DNA amplification journal June 2006
Rapid and Real-Time Detection of Chikungunya Virus by Reverse Transcription Loop-Mediated Isothermal Amplification Assay journal November 2006
Loop-Mediated Isothermal Amplification for Direct Detection of Mycobacterium tuberculosis Complex, M. avium, and M. intracellulare in Sputum Samples journal June 2003
Real-Time Reverse Transcription Loop-Mediated Isothermal Amplification for Rapid Detection of West Nile Virus journal January 2004
FRET-Based Assimilating Probe for Sequence-Specific Real-Time Monitoring of Loop-Mediated Isothermal Amplification (LAMP) journal January 2011
Loop-Mediated Isothermal Amplification (LAMP) Method for Rapid Detection of Trypanosoma brucei rhodesiense journal February 2008
Chikungunya: A Potentially Emerging Epidemic? journal April 2010
RNA and DNA Bacteriophages as Molecular Diagnosis Controls in Clinical Virology: A Comprehensive Study of More than 45,000 Routine PCR Tests journal February 2011
Detection of Acute HIV-1 Infection by RT-LAMP journal May 2015
Simultaneous multiple target detection in real-time loop-mediated isothermal amplification journal August 2012
Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue journal March 2009
Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes journal February 2015
Epidemiology and Transmission Dynamics of West Nile Virus Disease journal August 2005