RING Dimerization Links Higher-Order Assembly of TRIM5α to Synthesis of K63-Linked Polyubiquitin

Yudina, Zinaida; Roa, Amanda; Johnson, Rory; Biris, Nikolaos; de Souza Aranha Vieira, Daniel A.; Tsiperson, Vladislav; Reszka, Natalia; Taylor, Alexander B.; Hart, P. John; Demeler, Borries; Diaz-Griffero, Felipe; Ivanov, Dmitri N.

doi:10.1016/j.celrep.2015.06.072

Title: RING Dimerization Links Higher-Order Assembly of TRIM5α to Synthesis of K63-Linked Polyubiquitin

Journal Article · Sat Aug 01 00:00:00 EDT 2015 · Cell Reports

DOI:https://doi.org/10.1016/j.celrep.2015.06.072· OSTI ID:1242423

Yudina, Zinaida; Roa, Amanda; Johnson, Rory; Biris, Nikolaos; de Souza Aranha Vieira, Daniel A.; Tsiperson, Vladislav; Reszka, Natalia; Taylor, Alexander B.; Hart, P. John; Demeler, Borries; Diaz-Griffero, Felipe; Ivanov, Dmitri N.

Members of the tripartite motif (TRIM) protein family of RING E3 ubiquitin (Ub) ligases promote innate immune responses by catalyzing synthesis of polyubiquitin chains linked through lysine 63 (K63). Here, we investigate the mechanism by which the TRIM5α retroviral restriction factor activates Ubc13, the K63-linkage-specific E2. Structural, biochemical, and functional characterization of the TRIM5α:Ubc13-Ub interactions reveals that activation of the Ubc13-Ub conjugate requires dimerization of the TRIM5α RING domain. Our data explain how higher-order oligomerization of TRIM5α, which is promoted by the interaction with the retroviral capsid, enhances the E3 Ub ligase activity of TRIM5α and contributes to its antiretroviral function. This E3 mechanism, in which RING dimerization is transient and depends on the interaction of the TRIM protein with the ligand, is likely to be conserved in many members of the TRIM family and may have evolved to facilitate recognition of repetitive epitope patterns associated with infection.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Contributing Organization:: Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1242423

Alternate ID(s):: OSTI ID: 1208690

Journal Information:: Cell Reports, Journal Name: Cell Reports Vol. 12 Journal Issue: 5; ISSN 2211-1247

Publisher:: ElsevierCopyright Statement

Country of Publication:: Netherlands

Language:: English

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Cited by: 63 works

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