Quantitation of repaglinide and metabolites in mouse whole-body thin tissue sections using droplet-based liquid microjunction surface sampling-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry

Chen, Weiqi; Wang, Lifei; Van Berkel, Gary J.; Gan, Jinping; Kertesz, Vilmos

doi:10.1016/j.chroma.2015.10.093

Quantitation of repaglinide and metabolites in mouse whole-body thin tissue sections using droplet-based liquid microjunction surface sampling-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry

Journal Article · Tue Nov 03 00:00:00 EST 2015 · Journal of Chromatography

DOI:https://doi.org/10.1016/j.chroma.2015.10.093· OSTI ID:1241465

Chen, Weiqi ^[1]; Wang, Lifei ^[1]; Van Berkel, Gary J. ^[2]; Gan, Jinping ^[1]; Kertesz, Vilmos ^[2]

Bristol-Myers Squibb Pharmaceutical Research & Development, Princeton, NJ (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Herein, quantitation aspects of a fully automated autosampler/HPLC-MS/MS system applied for unattended droplet-based surface sampling of repaglinide dosed thin tissue sections with subsequent HPLC separation and mass spectrometric analysis of parent drug and various drug metabolites was studied. Major organs (brain, lung, liver, kidney, muscle) from whole-body thin tissue sections and corresponding organ homogenates prepared from repaglinide dosed mice were sampled by surface sampling and by bulk extraction, respectively, and analyzed by HPLC-MS/MS. A semi-quantitative agreement between data obtained by surface sampling and that by employing organ homogenate extraction was observed. Drug concentrations obtained by the two methods followed the same patterns for post-dose time points (0.25, 0.5, 1 and 2 h). Drug amounts determined in the specific tissues was typically higher when analyzing extracts from the organ homogenates. Furthermore, relative comparison of the levels of individual metabolites between the two analytical methods also revealed good semi-quantitative agreement.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1241465

Alternate ID(s):: OSTI ID: 1252496

Journal Information:: Journal of Chromatography, Journal Name: Journal of Chromatography Journal Issue: C Vol. 1439; ISSN 0021-9673

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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liquid microjunction
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