Microbial diversity and carbon cycling in San Francisco Bay wetlands

Conference · Fri Mar 21 04:00:00 EDT 2014

OSTI ID:1241184

Theroux, Susanna ^[1]; Hartman, Wyatt ^[1]; He, Shaomei ^[2]; Tringe, Susannah ^[1]

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1. Lawrence Berkeley National Lab. (LBNL), Walnut Creek, CA (United States). Dept. of Energy Joint Genome Inst.
2. Lawrence Berkeley National Lab. (LBNL), Walnut Creek, CA (United States). Dept. of Energy Joint Genome Inst.; Univ. of Wisconsin, Madison, WI (United States)

Wetland restoration efforts in San Francisco Bay aim to rebuild habitat for endangered species and provide an effective carbon storage solution, reversing land subsidence caused by a century of industrial and agricultural development. However, the benefits of carbon sequestration may be negated by increased methane production in newly constructed wetlands, making these wetlands net greenhouse gas (GHG) sources to the atmosphere. We investigated the effects of wetland restoration on below-ground microbial communities responsible for GHG cycling in a suite of historic and restored wetlands in SF Bay. Using DNA and RNA sequencing, coupled with real-time GHG monitoring, we profiled the diversity and metabolic potential of wetland soil microbial communities. The wetland soils harbor diverse communities of bacteria and archaea whose membership varies with sampling location, proximity to plant roots and sampling depth. Our results also highlight the dramatic differences in GHG production between historic and restored wetlands and allow us to link microbial community composition and GHG cycling with key environmental variables including salinity, soil carbon and plant species.

• Research Organization: Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (United States)
• Sponsoring Organization: USDOE Office of Science (SC)
• DOE Contract Number: AC02-05CH11231
• OSTI ID: 1241184
• Report Number(s): LBNL--7053E
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
99 GENERAL AND MISCELLANEOUS
DNA
GHG
RNA Sequencing