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Title: Evaluating models of cellulose degradation by Fibrobacter succinogenes S85

Journal Article · · PLoS ONE
 [1];  [1];  [2];  [1];  [1];  [2];  [1]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  2. Univ. of Wisconsin-Madison, Madison, WI (United States)
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Fibrobacter succinogenes S85 is an anaerobic non-cellulosome utilizing cellulolytic bacterium originally isolated from the cow rumen microbial community. Efforts to elucidate its cellulolytic machinery have resulted in the proposal of numerous models which involve a combination of cell-surface attachment via a combination of cellulose-binding fibro-slime proteins and pili, the production of cellulolytic vesicles, and the entry of cellulose fibers into the periplasmic space. Here, we used a combination of RNA-sequencing, proteomics, and transmission electron microscopy (TEM) to further elucidate the cellulolytic mechanism of F. succinogenes. Our RNA-sequence analysis shows that genes encoding Type II and III secretion systems, fibro-slime proteins, and pili are differentially expressed on cellulose, relative to glucose. A subcellular fractionation of cells grown on cellulose revealed that carbohydrate active enzymes associated with cellulose deconstruction and fibro-slime proteins were greater in the extracellular media, as compared to the periplasm and outer membrane fractions. TEMs of samples harvested at mid-exponential and stationary phases of growth on cellulose and glucose showed the presence of grooves in the cellulose between the bacterial cells and substrate, suggesting enzymes work extracellularly for cellulose degradation. Membrane vesicles were only observed in stationary phase cultures grown on cellulose. Furthermore, these results provide evidence that F. succinogenes attaches to cellulose fibers using fibro-slime and pili, produces cellulases, such as endoglucanases, that are secreted extracellularly using type II and III secretion systems, and degrades the cellulose into cellodextrins that are then imported back into the periplasm for further digestion by β-glucanases and other cellulases.

Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Organization:
USDOE
Grant/Contract Number:
AC05-76RL01830
OSTI ID:
1241081
Report Number(s):
PNNL-SA-111279; 34708; 47418; 48680; KP1601010
Journal Information:
PLoS ONE, Vol. 10, Issue 12; ISSN 1932-6203
Publisher:
Public Library of ScienceCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 31 works
Citation information provided by
Web of Science

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Cited By (11)

Enzymes for Bioenergy book January 2017
The unusual cellulose utilization system of the aerobic soil bacterium Cytophaga hutchinsonii journal August 2017
Profiling microbial lignocellulose degradation and utilization by emergent omics technologies journal July 2016
Islands in the stream: from individual to communal fiber degradation in the rumen ecosystem journal April 2019
Outer membrane vesicles from Fibrobacter succinogenes S85 contain an array of carbohydrate-active enzymes with versatile polysaccharide-degrading capacity journal May 2017
Lignocellulose-Degrading Microbial Communities in Landfill Sites Represent a Repository of Unexplored Biomass-Degrading Diversity journal August 2017
A global analysis of gene expression in Fibrobacter succinogenes S85 grown on cellulose and soluble sugars at different growth rates journal October 2018
Extracellular vesicles carry cellulases in the industrial fungus Trichoderma reesei journal June 2019
“Candidatus Paraporphyromonas polyenzymogenes” encodes multi-modular cellulases linked to the type IX secretion system journal March 2018
Metatranscriptomics Reveals the Active Bacterial and Eukaryotic Fibrolytic Communities in the Rumen of Dairy Cow Fed a Mixed Diet journal January 2017
Deciphering the unique cellulose degradation mechanism of the ruminal bacterium Fibrobacter succinogenes S85 journal November 2019

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59 BASIC BIOLOGICAL SCIENCES
Environmental Molecular Sciences Laboratory
cellulose
outer membrane proteins
glucose
vesicles
periplasm
carbohydrates
cellulases
cell binding