The kinase LYK5 is a major chitin receptor in Arabidopsis and forms a chitin-induced complex with related kinase CERK1

Cao, Yangrong; Liang, Yan; Tanaka, Kiwamu; Nguyen, Cuong T.; Jedrzejczak, Robert P.; Joachimiak, Andrzej; Stacey, Gary

doi:10.7554/eLife.03766

Title: The kinase LYK5 is a major chitin receptor in Arabidopsis and forms a chitin-induced complex with related kinase CERK1

Journal Article · Thu Oct 23 00:00:00 EDT 2014 · eLife

DOI:https://doi.org/10.7554/eLife.03766· OSTI ID:1181086

Cao, Yangrong ^[1]; Liang, Yan ^[1];

^[1]; Nguyen, Cuong T. ^[1]; Jedrzejczak, Robert P. ^[2]; Joachimiak, Andrzej ^[2]; Stacey, Gary ^[1]

Division of Plant Sciences, National Center for Soybean Biotechnology, University of Missouri, Columbia, United States, Department of Biochemistry, University of Missouri, Columbia, United States
Midwest Center for Structural Genomics, Argonne National Laboratory, Argonne, United States

Chitin is a fungal microbe-associated molecular pattern (MAMP) that is recognized in Arabidopsis by a lysin motif receptor kinase (LYK), AtCERK1. Previous research suggested that AtCERK1 is the major chitin receptor in plants and mediates chitin-induced signaling through homodimerization and phosphorylation. However, the reported chitin binding affinity of AtCERK1 is quite low, suggesting another receptor with high chitin binding affinity might be present. Here, we propose that AtLYK5 is the primary chitin receptor in Arabidopsis. Mutations in AtLYK5 resulted in a significant reduction in the plant chitin response. However, AtLYK5 shares overlapping function with AtLYK4 and, therefore, only AtLYK4/AtLYK5-2 double mutants show a complete loss of chitin response. AtLYK5 interacts with AtCERK1 in a chitin-dependent manner. Chitin binding to AtLYK5 is indispensable for chitin-induced AtCERK1 phosphorylation. AtLYK5 binds chitin at a much higher affinity than AtCERK1. Furthermore, the data suggest that AtLYK5 is the primary plant receptor for chitin, forming a chitin inducible complex with AtCERK1 to induce plant innate immunity.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: FG02-08ER15309; FG02-02ER15309; AC02-06CH11357

OSTI ID:: 1181086

Alternate ID(s):: OSTI ID: 1227909; OSTI ID: 1240691

Journal Information:: eLife, Journal Name: eLife Vol. 3; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 371 works

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