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Title: Morphological and chemical changes of aerosolized E. coli treated with a dielectric barrier discharge

Journal Article · · Biointerphases
DOI:https://doi.org/10.1116/1.4941367· OSTI ID:1240000
 [1];  [2];  [3];  [3];  [3];  [4];  [5];  [5];  [1];  [1];  [1];  [1];  [6]
  1. NASA Ames Research Center, Moffett Field, CA (United States)
  2. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  3. Novaerus, Inc., Chicago, IL (United States)
  4. Aalto Univ., Espoo (Finland)
  5. Dublin City Univ., Dublin (Ireland)
  6. NASA Ames Research Center, Moffett Field, CA (United States); Univ. Space Research Association, Mountain View, CA (United States)
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This paper presents the morphological and chemical modification of the cell structure of aerosolized Escherichia coli treated with a dielectric barrier discharge (DBD). Exposure to DBD results in severe oxidation of the bacteria, leading to the formation of hydroxyl groups and carbonyl groups and a significant reduction in amine functionalities and phosphate groups. Near edge x-ray absorption fine structure(NEXAFS) measurements confirm the presence of additional oxide bonds upon DBD treatment, suggesting oxidation of the outer layer of the cell wall. Electron microscopy images show that the bacteria undergo physical distortion to varying degrees, resulting in deformation of the bacterial structure. The electromagnetic field around the DBD coil causes severe damage to the cell structure, possibly resulting in leakage of vital cellular materials. The oxidation and chemical modification of the bacterial components are evident from the Fourier transform infrared spectroscopy and NEXAFS results. The bacterial reculture experiments confirm inactivation of airborne E. coli upon treating with DBD.

Research Organization:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
Grant/Contract Number:
AC02-76SF00515
OSTI ID:
1240000
Journal Information:
Biointerphases, Vol. 11, Issue 1; ISSN 1934-8630
Publisher:
AIPCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 7 works
Citation information provided by
Web of Science

Cited By (1)

Atmospheric Pressure Pulsed Plasma Induces Cell Death in Photosynthetic Organs via Intracellularly Generated ROS journal April 2017

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
bacteria
X-ray absorption spectroscopy
X-ray absorption near edge structure
carbon
Fourier transform infrared spectroscopy