The role of pore geometry in single nanoparticle detection

Davenport, Matthew; Healy, Ken; Pevarnik, Matthew; Teslich, Nick; Cabrini, Stefano; Morrison, Alan P.; Siwy, Zuzanna S.; Letant, Sonia E.

doi:10.1021/nn303126n

The role of pore geometry in single nanoparticle detection

Journal Article · Wed Aug 22 00:00:00 EDT 2012 · ACS Nano

DOI:https://doi.org/10.1021/nn303126n· OSTI ID:1239207

Davenport, Matthew ^[1]; Healy, Ken ^[2]; Pevarnik, Matthew ^[3]; Teslich, Nick ^[4]; Cabrini, Stefano ^[5]; Morrison, Alan P. ^[6]; Siwy, Zuzanna S. ^[3]; Letant, Sonia E. ^[4]

Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Univ. of California, Irvine, CA (United States)
Univ. of California, Irvine, CA (United States); Univ. College Cork (Ireland)
Univ. of California, Irvine, CA (United States)
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Univ. College Cork (Ireland)

In this study, we observe single nanoparticle translocation events via resistive pulse sensing using silicon nitride pores described by a range of lengths and diameters. Pores are prepared by focused ion beam milling in 50 nm-, 100 nm-, and 500 nm-thick silicon nitride membranes with diameters fabricated to accommodate spherical silica nanoparticles with sizes chosen to mimic that of virus particles. In this manner, we are able to characterize the role of pore geometry in three key components of the detection scheme, namely, event magnitude, event duration, and event frequency. We find that the electric field created by the applied voltage and the pore’s geometry is a critical factor. We develop approximations to describe this field, which are verified with computer simulations, and interactions between particles and this field. In so doing, we formulate what we believe to be the first approximation for the magnitude of ionic current blockage that explicitly addresses the invariance of access resistance of solid-state pores during particle translocation. These approximations also provide a suitable foundation for estimating the zeta potential of the particles and/or pore surface when studied in conjunction with event durations. We also verify that translocation achieved by electro-osmostic transport is an effective means of slowing translocation velocities of highly charged particles without compromising particle capture rate as compared to more traditional approaches based on electrophoretic transport.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1239207

Report Number(s):: LLNL-JRNL--564494

Journal Information:: ACS Nano, Journal Name: ACS Nano Journal Issue: 9 Vol. 6; ISSN 1936-0851

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

References (45)

Submicrometer Pore-Based Characterization and Quantification of Antibody–Virus Interactions Uram, Jeffrey D.; Ke, Kevin; Hunt, Alan J. Small, Vol. 2, Issue 8-9 https://doi.org/10.1002/smll.200600006	journal	August 2006
Fabrication of Metallized Nanopores in Silicon Nitride Membranes for Single-Molecule Sensing Wei, Ruoshan; Pedone, Daniel; Zürner, Andreas Small, Vol. 6, Issue 13 https://doi.org/10.1002/smll.201000253	journal	June 2010
Electrokinetic measurements with submicron particles and pores by the resistive pulse technique DeBlois, Ralph W.; Bean, Charles P.; Wesley, Roy K. A. Journal of Colloid and Interface Science, Vol. 61, Issue 2, p. 323-335 https://doi.org/10.1016/0021-9797(77)90395-2	journal	September 1977
The zeta potential of silicon nitride thin films Bousse, Luc; Mostarshed, Shahriar Journal of Electroanalytical Chemistry and Interfacial Electrochemistry, Vol. 302, Issue 1-2 https://doi.org/10.1016/0022-0728(91)85046-R	journal	March 1991
Electrical Counting and Sizing of Mammalian Cells in Suspension Gregg, E. C.; Steidley, K. David Biophysical Journal, Vol. 5, Issue 4 https://doi.org/10.1016/S0006-3495(65)86724-8	journal	July 1965
Nanopores: A journey towards DNA sequencing Wanunu, Meni Physics of Life Reviews, Vol. 9, Issue 2 https://doi.org/10.1016/j.plrev.2012.05.010	journal	June 2012
Simultaneous Determination of the Size and Surface Charge of Individual Nanoparticles Using a Carbon Nanotube-Based Coulter Counter Ito, Takashi; Sun, Li; Crooks, Richard M. Analytical Chemistry, Vol. 75, Issue 10 https://doi.org/10.1021/ac034072v	journal	May 2003
Nanoparticle Transport in Conical-Shaped Nanopores Lan, Wen-Jie; Holden, Deric A.; Zhang, Bo Analytical Chemistry, Vol. 83, Issue 10 https://doi.org/10.1021/ac200312n	journal	May 2011
Resistive-Pulse Sensing - From Microbes to Molecules Bayley, Hagan; Martin, Charles R. Chemical Reviews, Vol. 100, Issue 7, p. 2575-2594 https://doi.org/10.1021/cr980099g	journal	July 2000
Single Carbon Nanotube Membranes: A Well-Defined Model for Studying Mass Transport through Nanoporous Materials Sun, Li; Crooks, Richard M. Journal of the American Chemical Society, Vol. 122, Issue 49, p. 12340-12345 https://doi.org/10.1021/ja002429w	journal	December 2000
Characterization of Hepatitis B Virus Capsids by Resistive-Pulse Sensing Zhou, Kaimeng; Li, Lichun; Tan, Zhenning Journal of the American Chemical Society, Vol. 133, Issue 6 https://doi.org/10.1021/ja108228x	journal	February 2011
Dynamics of Colloids in Single Solid-State Nanopores Bacri, L.; Oukhaled, A. G.; Schiedt, B. The Journal of Physical Chemistry B, Vol. 115, Issue 12 https://doi.org/10.1021/jp200326w	journal	March 2011
Pressure-Driven Nanoparticle Transport across Glass Membranes Containing a Conical-Shaped Nanopore Lan, Wen-Jie; Holden, Deric A.; Liu, Jin The Journal of Physical Chemistry C, Vol. 115, Issue 38 https://doi.org/10.1021/jp204839j	journal	August 2011
Comparison of Nanoparticle Size and Electrophoretic Mobility Measurements Using a Carbon-Nanotube-Based Coulter Counter, Dynamic Light Scattering, Transmission Electron Microscopy, and Phase Analysis Light Scattering Ito, Takashi; Sun, Li; Bevan, Michael A. Langmuir, Vol. 20, Issue 16 https://doi.org/10.1021/la049524t	journal	August 2004
Fabrication and Characterization of Single Pores for Modeling Mass Transport across Porous Membranes Sun, Li; Crooks, Richard M. Langmuir, Vol. 15, Issue 3 https://doi.org/10.1021/la980871a	journal	February 1999
Electrically Facilitated Translocations of Proteins through Silicon Nitride Nanopores: Conjoint and Competitive Action of Diffusion, Electrophoresis, and Electroosmosis Firnkes, Matthias; Pedone, Daniel; Knezevic, Jelena Nano Letters, Vol. 10, Issue 6 https://doi.org/10.1021/nl100861c	journal	June 2010
Translocation of Single-Wall Carbon Nanotubes Through Solid-State Nanopores Hall, Adam R.; Keegstra, Johannes M.; Duch, Matthew C. Nano Letters, Vol. 11, Issue 6 https://doi.org/10.1021/nl200873w	journal	June 2011
Detection of Nucleosomal Substructures using Solid-State Nanopores Soni, Gautam V.; Dekker, Cees Nano Letters, Vol. 12, Issue 6 https://doi.org/10.1021/nl301163m	journal	May 2012
Ionic Field Effect Transistors with Sub-10 nm Multiple Nanopores Nam, Sung-Wook; Rooks, Michael J.; Kim, Ki-Bum Nano Letters, Vol. 9, Issue 5 https://doi.org/10.1021/nl900309s	journal	May 2009
Distinguishing Single- and Double-Stranded Nucleic Acid Molecules Using Solid-State Nanopores Skinner, Gary M.; van den Hout, Michiel; Broekmans, Onno Nano Letters, Vol. 9, Issue 8 https://doi.org/10.1021/nl901370w	journal	August 2009
Gate Manipulation of DNA Capture into Nanopores He, Yuhui; Tsutsui, Makusu; Fan, Chun ACS Nano, Vol. 5, Issue 10 https://doi.org/10.1021/nn203186c	journal	September 2011
Diffusional Motion of a Particle Translocating through a Nanopore Lan, Wen-Jie; White, Henry S. ACS Nano, Vol. 6, Issue 2 https://doi.org/10.1021/nn2047636	journal	January 2012
Single-Nanoparticle Detection Using a Low-Aspect-Ratio Pore Tsutsui, Makusu; Hongo, Sadato; He, Yuhui ACS Nano, Vol. 6, Issue 4 https://doi.org/10.1021/nn300530b	journal	March 2012
Simultaneous Size and ζ-Potential Measurements of Individual Nanoparticles in Dispersion Using Size-Tunable Pore Sensors Kozak, Darby; Anderson, Will; Vogel, Robert ACS Nano, Vol. 6, Issue 8 https://doi.org/10.1021/nn3020322	journal	July 2012
Polystyrene Particles Reveal Pore Substructure As They Translocate Pevarnik, Matthew; Healy, Ken; Toimil-Molares, Maria Eugenia ACS Nano, Vol. 6, Issue 8 https://doi.org/10.1021/nn302413u	journal	July 2012
Electroosmotic Flow Can Generate Ion Current Rectification in Nano- and Micropores Yusko, Erik C.; An, Ran; Mayer, Michael ACS Nano, Vol. 4, Issue 1 https://doi.org/10.1021/nn9013438	journal	December 2009
Inverse Statistical Variates Tweedie, M. C. K. Nature, Vol. 155, Issue 3937 https://doi.org/10.1038/155453a0	journal	April 1945
The potential and challenges of nanopore sequencing Branton, Daniel; Deamer, David W.; Marziali, Andre Nature Biotechnology, Vol. 26, Issue 10 https://doi.org/10.1038/nbt.1495	journal	October 2008
Automated forward and reverse ratcheting of DNA in a nanopore at 5-Å precision Cherf, Gerald M.; Lieberman, Kate R.; Rashid, Hytham Nature Biotechnology, Vol. 30, Issue 4 https://doi.org/10.1038/nbt.2147	journal	February 2012
Reading DNA at single-nucleotide resolution with a mutant MspA nanopore and phi29 DNA polymerase Manrao, Elizabeth A.; Derrington, Ian M.; Laszlo, Andrew H. Nature Biotechnology, Vol. 30, Issue 4 https://doi.org/10.1038/nbt.2171	journal	March 2012
Solid-state nanopores Dekker, Cees Nature Nanotechnology, Vol. 2, Issue 4 https://doi.org/10.1038/nnano.2007.27	journal	March 2007
Electrostatic focusing of unlabelled DNA into nanoscale pores using a salt gradient Wanunu, Meni; Morrison, Will; Rabin, Yitzhak Nature Nanotechnology, Vol. 5, Issue 2 https://doi.org/10.1038/nnano.2009.379	journal	December 2009
Nanopore sensors for nucleic acid analysis Venkatesan, Bala Murali; Bashir, Rashid Nature Nanotechnology, Vol. 6, Issue 10 https://doi.org/10.1038/nnano.2011.129	journal	September 2011
Quantitative sensing of nanoscale colloids using a microchip Coulter counter Saleh, O. A.; Sohn, L. L. Review of Scientific Instruments, Vol. 72, Issue 12 https://doi.org/10.1063/1.1419224	journal	December 2001
Counting and Sizing of Submicron Particles by the Resistive Pulse Technique DeBlois, R. W.; Bean, C. P. Review of Scientific Instruments, Vol. 41, Issue 7 https://doi.org/10.1063/1.1684724	journal	July 1970
Sensing protein molecules using nanofabricated pores Han, Anpan; Schürmann, Gregor; Mondin, Giampietro Applied Physics Letters, Vol. 88, Issue 9 https://doi.org/10.1063/1.2180868	journal	February 2006
Polymer capture by electro-osmotic flow of oppositely charged nanopores Wong, C. T. A.; Muthukumar, M. The Journal of Chemical Physics, Vol. 126, Issue 16 https://doi.org/10.1063/1.2723088	journal	April 2007
Characterization of individual polynucleotide molecules using a membrane channel Kasianowicz, J. J.; Brandin, E.; Branton, D. Proceedings of the National Academy of Sciences, Vol. 93, Issue 24 https://doi.org/10.1073/pnas.93.24.13770	journal	November 1996
Access resistance of a small circular pore Hall, J. E. The Journal of General Physiology, Vol. 66, Issue 4 https://doi.org/10.1085/jgp.66.4.531	journal	October 1975
Transport phenomena in nanofluidics Schoch, Reto B.; Han, Jongyoon; Renaud, Philippe Reviews of Modern Physics, Vol. 80, Issue 3 https://doi.org/10.1103/RevModPhys.80.839	journal	July 2008
Sizes and concentrations of several type C oncornaviruses and bacteriophage T2 by the resistive-pulse technique. DeBlois, R. W.; Wesley, R. K. Journal of Virology, Vol. 23, Issue 2 https://doi.org/10.1128/JVI.23.2.227-233.1977	journal	January 1977
Sizes and concentrations of several type C oncornaviruses and bacteriophage T2 by the resistive-pulse technique. DeBlois, R. W.; Wesley, R. K. Journal of Virology, Vol. 23, Issue 2 https://doi.org/10.1128/jvi.23.2.227-233.1977	journal	January 1977
DNA Translocation Governed by Interactions with Solid-State Nanopores Wanunu, Meni; Sutin, Jason; McNally, Ben Biophysical Journal, Vol. 95, Issue 10 https://doi.org/10.1529/biophysj.108.140475	journal	November 2008
Nanopore-based single-molecule DNA analysis Healy, Ken Nanomedicine, Vol. 2, Issue 4 https://doi.org/10.2217/17435889.2.4.459	journal	August 2007
Solid-state nanopore technologies for nanopore-based DNA analysis Healy, Ken; Schiedt, Birgitta; Morrison, Alan P. Nanomedicine, Vol. 2, Issue 6 https://doi.org/10.2217/17435889.2.6.875	journal	December 2007

Cited By (23)

Nanopore-based analysis of biochemical species Liu, Nannan; Yang, Zekun; Ou, Xiaowen Microchimica Acta, Vol. 183, Issue 3 https://doi.org/10.1007/s00604-015-1560-2	journal	July 2015
Single-Particle Electrophoresis in Nanochannels Harms, Zachary D.; Haywood, Daniel G.; Kneller, Andrew R. Analytical Chemistry, Vol. 87, Issue 1 https://doi.org/10.1021/ac503527d	journal	December 2014
Discriminating single-bacterial shape using low-aspect-ratio pores Tsutsui, Makusu; Yoshida, Takeshi; Yokota, Kazumichi Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-17443-6	journal	December 2017
Volume discrimination of nanoparticles via electrical trapping using nanopores Arima, Akihide; Tsutsui, Makusu; Taniguchi, Masateru Journal of Nanobiotechnology, Vol. 17, Issue 1 https://doi.org/10.1186/s12951-019-0471-5	journal	March 2019
Microfluidic and Nanofluidic Resistive Pulse Sensing: A Review Song, Yongxin; Zhang, Junyan; Li, Dongqing Micromachines, Vol. 8, Issue 7 https://doi.org/10.3390/mi8070204	journal	June 2017
Probing Dynamic Events of Dielectric Nanoparticles by a Nanoelectrode‐Nanopore Nanopipette Pandey, Popular; Panday, Namuna; Chang, Shuai ChemElectroChem, Vol. 5, Issue 20 https://doi.org/10.1002/celc.201800163	journal	May 2018
Low aspect ratio micropores for single-particle and single-cell analysis: Nanoanalysis Goyal, Gaurav; Mulero, Rafael; Ali, Jamel ELECTROPHORESIS, Vol. 36, Issue 9-10 https://doi.org/10.1002/elps.201400570	journal	April 2015
Voltage-Gated Nanoparticle Transport and Collisions in Attoliter-Volume Nanopore Electrode Arrays Fu, Kaiyu; Han, Donghoon; Crouch, Garrison M. Small, Vol. 14, Issue 18 https://doi.org/10.1002/smll.201703248	journal	January 2018
Detection of Individual Molecules and Ions by Carbon Nanotube-Based Differential Resistive Pulse Sensor Peng, Ran; Tang, Xiaowu Shirley; Li, Dongqing Small, Vol. 14, Issue 15 https://doi.org/10.1002/smll.201800013	journal	March 2018
Nanopore‐Based Protein Sequencing Using Biopores: Current Achievements and Open Challenges Asandei, Alina; Di Muccio, Giovanni; Schiopu, Irina Small Methods, Vol. 4, Issue 11 https://doi.org/10.1002/smtd.201900595	journal	February 2020
Electrokinetic motion of single nanoparticles in single PDMS nanochannels Peng, Ran; Li, Dongqing Microfluidics and Nanofluidics, Vol. 21, Issue 1 https://doi.org/10.1007/s10404-017-1848-0	journal	January 2017
Tracking single-particle dynamics via combined optical and electrical sensing Yukimoto, Naoya; Tsutsui, Makusu; He, Yuhui Scientific Reports, Vol. 3, Issue 1 https://doi.org/10.1038/srep01855	journal	May 2013
Effects of access resistance on the resistive-pulse caused by translocating of a nanoparticle through a nanopore Wang, Junrong; Ma, Jian; Ni, Zhonghua RSC Advances, Vol. 4, Issue 15 https://doi.org/10.1039/c3ra46032k	journal	January 2014
Dynamics of polymer nanoparticles through a single artificial nanopore with a high-aspect-ratio Cabello-Aguilar, Simon; Chaaya, Adib Abou; Bechelany, Mikhael Soft Matter, Vol. 10, Issue 42 https://doi.org/10.1039/c4sm00392f	journal	January 2014
Optimal voltage for nanoparticle detection with thin nanopores Qiu, Yinghua The Analyst, Vol. 143, Issue 19 https://doi.org/10.1039/c8an01270a	journal	January 2018
Surface coatings for solid-state nanopores Eggenberger, Olivia M.; Ying, Cuifeng; Mayer, Michael Nanoscale, Vol. 11, Issue 42 https://doi.org/10.1039/c9nr05367k	journal	January 2019
Characterization and manipulation of single nanoparticles using a nanopore-based electrokinetic tweezer Yazbeck, Rami; Alibakhshi, Mohammad Amin; Von Schoppe, Joseph Nanoscale, Vol. 11, Issue 47 https://doi.org/10.1039/c9nr08476b	journal	January 2019
Rapid structural analysis of nanomaterials in aqueous solutions Ryuzaki, Sou; Tsutsui, Makusu; He, Yuhui Nanotechnology, Vol. 28, Issue 15 https://doi.org/10.1088/1361-6528/aa5e66	journal	March 2017
Controlled size reduction and its underlying mechanism to form solid-state nanopores via electron beam induced carbon deposition Zeng, Shuangshuang; Wen, Chenyu; Li, Shiyu Nanotechnology, Vol. 30, Issue 45 https://doi.org/10.1088/1361-6528/ab39a2	journal	August 2019
Construction of biomimetic smart nanochannels for confined water Wen, Liping; Jiang, Lei National Science Review, Vol. 1, Issue 1 https://doi.org/10.1093/nsr/nwt001	journal	November 2013
The raspberry model for protein-like particles: Ellipsoids and confinement in cylindrical pores Ustach, Vincent D.; Faller, Roland The European Physical Journal Special Topics, Vol. 225, Issue 8-9 https://doi.org/10.1140/epjst/e2016-60089-7	journal	October 2016
The Raspberry model for protein-like particles: ellipsoids and confinement in cylindrical pores Ustach, Vincent; Faller, Roland arXiv https://doi.org/10.48550/arxiv.1604.01829	text	January 2016
Optimal Voltage for Nanoparticle Detection with Thin Nanopores Qiu, Yinghua arXiv https://doi.org/10.48550/arxiv.1808.09498	text	January 2018

Similar Records

Molecular-scale studies of single-channel membrane pores : final report.

Technical Report · Wed Oct 01 00:00:00 EDT 2003 · OSTI ID:918367

Stretching and unzipping nucleic acid hairpins using a synthetic nanopore

Journal Article · Sun Jan 20 19:00:00 EST 2008 · Nucleic Acids Research · OSTI ID:1625417

Enhanced electro-osmosis in propylene carbonate salt solutions

Journal Article · Sun Apr 04 20:00:00 EDT 2021 · Journal of Chemical Physics · OSTI ID:1852831

Related Subjects

77 NANOSCIENCE AND NANOTECHNOLOGY
aspect ratio
ion current
nanoparticle
nanopore
resistive-pulse

The role of pore geometry in single nanoparticle detection

Citation Formats

References (45)

Cited By (23)

Similar Records

Related Subjects