Accounting for all sugars produced during integrated production of ethanol from lignocellulosic biomass

Schell, Daniel J.; Dowe, Nancy; Chapeaux, Alexandre; Nelson, Robert S.; Jennings, Edward W.

doi:10.1016/j.biortech.2016.01.024

Accounting for all sugars produced during integrated production of ethanol from lignocellulosic biomass

Journal Article · Tue Jan 19 00:00:00 EST 2016 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2016.01.024· OSTI ID:1237439

Schell, Daniel J. ^[1]; Dowe, Nancy ^[1]; Chapeaux, Alexandre ^[2]; Nelson, Robert S. ^[1]; Jennings, Edward W. ^[1]

National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center
P2 Science, Woodbridge, CT (United States)

This study explored integrated conversion of corn stover to ethanol and highlights techniques for accurate yield calculations. Acid pretreated corn stover (PCS) produced in a pilot-scale reactor was enzymatically hydrolyzed and the resulting sugars were fermented to ethanol by the glucose–xylose fermenting bacteria, Zymomonas mobilis 8b. The calculations account for high solids operation and oligomeric sugars produced during pretreatment, enzymatic hydrolysis, and fermentation, which, if not accounted for, leads to overestimating ethanol yields. The calculations are illustrated for enzymatic hydrolysis and fermentation of PCS at 17.5% and 20.0% total solids achieving 80.1% and 77.9% conversion of cellulose and xylan to ethanol and ethanol titers of 63 g/L and 69 g/L, respectively. In the future, these techniques, including the TEA results, will be applied to fully integrated pilot-scale runs.

Research Organization:: National Renewable Energy Lab. (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE; USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1237439

Alternate ID(s):: OSTI ID: 1358840

Report Number(s):: NREL/JA--5100-65494

Journal Information:: Bioresource Technology, Journal Name: Bioresource Technology Journal Issue: C Vol. 205; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Zymomonas mobilis as a model system for production of biofuels and biochemicals Yang, Shihui; Fei, Qiang; Zhang, Yaoping Microbial Biotechnology, Vol. 9, Issue 6 https://doi.org/10.1111/1751-7915.12408	journal	September 2016
Enzyme production and oil palm empty fruit bunch bioconversion to ethanol using a hybrid yeast strain Zhai, Lili; Manglekar, Rupali Rahul; Geng, Anli Biotechnology and Applied Biochemistry, Vol. 67, Issue 5 https://doi.org/10.1002/bab.1816	journal	September 2019
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Enhanced lipid production by Rhodosporidium toruloides using different fed-batch feeding strategies with lignocellulosic hydrolysate as the sole carbon source Fei, Qiang; O’Brien, Marykate; Nelson, Robert Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0542-x	journal	June 2016
Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis Yang, Yongfu; Hu, Mimi; Tang, Ying Bioresources and Bioprocessing, Vol. 5, Issue 1 https://doi.org/10.1186/s40643-018-0193-9	journal	February 2018

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09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
corn stover
enzymatic hydrolysis
ethanol
fermentation
mass balance
pretreatment
yields

Accounting for all sugars produced during integrated production of ethanol from lignocellulosic biomass

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